GENITOURINARY MYIASIS CAUSED BY MUSCA DOMESTICA: REGARDING A SINGULAR

A 27-year-old woman from and resident in ourika(Marrakesh region – Morocco) with a history of anemia under iron treatment, without a history of surgery, has G1 P1. Presented to the Mohamed VI University Hospital on day 21 post partum of a vaginal birth at home, the evolution was marked by the appearance of urinary incontinence treated in the emergency room by placing a catheter urinary on day 6 post partum then send to her home for the COVID 19 context, then the patient consulted again at the gyneco-obstetric emergency room for deterioration of the general condition in a picture of hemodynamic shock. On physical examination, the patient was hemodynamically unstable with blood pressure figures of 70/40 mmhg, heart rate at 50 bpm, temperature at 35 ° and mucosal skin pallor.Examination of the vaginal cavity showed the presence of numerous whitish-looking maggots, a sample was taken by the biology team that collected the maggots for the purpose of a parasitological study to identify the parasite responsible.

The helminth fauna of Pontic shad (Alosa Immacculata Bennet, 1835) from lower Dniester

In this study 60 specimens of Pontic were caught from lower Dniester, Stefan Voda district, Olănesti. As a result of the parasitological study of Pontic shad, three species of helminths were detected: Pronoprymna ventricosa (Rudolphi, 1819) Poche, 1926, Lecithasther confusus Odhner, 1905), Hysterothylacium aduncum (Rudolphi, 1802). The degree of infestation with these species was different. The most abundant species was Hysterothylacium aduncum with an extensivity of invasion of 85% and intensivity of invasion 1 – 80 parasites per fish, followed by Pronoprymna ventricosa (EI-20%, II-2-18 ex.) and Lecythaster confusus (EI-7,8%, II-1-12 ex.).

Parasite fauna of Javanese macaques (Macaca fascicularis)

This paper presents an analysis of the parasite fauna of Javanese macaques kept in captivity in the conditions of the Adler nursery of the FGBNU «NII MP». In the course of the parasitological study, 245 Macaca fascicularis monkeys were examined. As a result, it was revealed that they have 4 varieties of worms of the nematode class: Ancylostoma spp., Trichoceephalus spp., Strongyloides spp., Oesophagostomum spp., As well as 3 species of protozoa: Balantidium coli, Blastocystis spp. and Lamblia intestinalis. During the analysis of the data obtained, it was found that the total invasion of the studied animals was 44.5%. It was found that the most common types of helminths found in the studied animals are Strongyloides spp., Parasitizing in the duodenum and upper jejunum of mammals, the extent of invasion was 6.9%. Of the protozoa, the species Balantidium coli, parasitizing in mammals in the large intestine, was most often found; the extent of invasion was 18.8%. The extensiveness of the invasion of Ancylostoma spp., Localized in the small intestine, in the studied cynomolgus macaques was 5.7%, Blastocystis spp., Found in the cecum and proximal colon, 2.9%, Trichoceephalus spp., Parasitizing in the blind part of the intestine, – 3.3%, Oesophagostomum spp., penetrating into the mucous membrane and submucosa of the large intestine, – 0.8%. Also, the studied macaques were found to have combined invasions. Extensiveness of invasion of Balantidium coli simultaneously with Strongyloides spp. was 0.8%, Balantidium coli simultaneously with Trichoceephalus spp. – 1.2%, Balantidium coli simultaneously with Ancylostoma spp. – 0.4%, Balantidium coli simultaneously with Blastocystis sp. – 0.4%, Balantidium coli simultaneously with Oesophagostomum spp. and Trichoceephalus spp. – 0.4%, Strongyloides spp. simultaneously with Lamblia intestinalis – 0.4%, Strongyloides spp. simultaneously with Trichoceephalus spp. – 1.2%, Blastocystis sp. simultaneously with Ancylostoma spp. – 0.4%, Blastocystis spp. simultaneously with Trichoceephalus spp. – 0.4%, Trichoceephalus spp. simultaneously with Ancylostoma spp. – 0.4%.

Endo-Helminth Fauna of the Rainbow Lizard (Agama Agama)

This parasitological study was carried out between February and May 2019 to determine the prevalence and intensity of helminthiasis in the rainbow lizard (Agama agama) in Okrika, Rivers State, Nigeria. A total of one hundred and fifty-one (151) specimens made up of 93 males and 75 females were caught by a local netting system during the day and anaesthesized with chloroform. Samples were collected from two stations (Ogoloma-Ama and Oba-Ama). After dissection, the esophagus, stomach, small intestine, rectum, liver, lungs, urinary bladder, heart and body cavity were searched for helminths using conventional methods. Five species of helminths belonging to Nematoda - Strongyluris brevicaudata, Parapharyngodon awokoyai, encysted Ascaridida larva, Cestoda - Oochoristica sp. and Trematoda - Mesocoelium spp - were recovered from infected lizards. Helminths infected one hundred and twenty-three (123) (82%) of the lizards. In Oba-Ama, forty-five (45) (76.3%) out of 59 and in Ogoloma-Ama, seventy-eight (78) (84.8%) out of 92 were infected with helminths. By abundance, in both locations, the males were more infected than their female counterparts with a prevalence of 51(93%) and 72(75%) (P<0.05), respectively. This study has revealed the helminth parasites infecting the agamid lizard of Rivers State, Nigeria. It has also shown some unidentified species of Mesocoelium and Oochoristica sp. Additionally the trapping system used was also found to be effective and efficient.

Endoparasic diseases of ostriches in eastern Ukraine

In ostrich farming is difficult to obtain the necessary production and economic results without protection of bird health. We determined the incidence of parasitic ostriches during the period of growing and keeping on specialized eco-zoos. Samples from small and large farms, as well as eco-parks in Kharkiv, Dnepr and Donetsk regions of Ukraine, were examined during 2016- 2020. We performed a parasitological study of 159 samples from three species of ostriches (black African ostrich, Australian common Emu (Dromaius), Rhea). Infestation of ostriches of different ages with two types of nematodes and Eimeria was established. We determoined that the population of ostriches in specialized farms and eco-zoos was infested with nematodes and protozoa with an extensiveness of invasion from 7.7% to 71.4% among adult birds, while young ostriches were infested with Emeria from 20.0% to 44.4%. The largest invasion by helminths and protozoa was observed among black African ostriches in Kharkiv region (41.4%), in Dnepr region it was 33.3%, and in Donetsk it reached 34.1%. Among the livestock of ostriches, there were both mono and mixinvasions. Most often, this was an invasion by Trichostrongylus spp. and Eimeria spp. in African black ostriches, capillaries and Eimeria in Emu. In Kharkiv region, the invasion of Capillaria by Trichostrongylus in African ostriches and Trichostrongylus in Emu were found when they were kept together. Acute and chronic eimeriosis disease can lead to the death of ostriches at the age of 3-4 months.

MONITORING OF DOG DIROFILARIASIS THE NORTH-WESTERN CAUCASUS

In the course of a study conducted in 2015–2019, it was found that there is a permanent center of dirofilariasis in the North-Western Caucasus, which is actively supported by domestic dogs and cats, as well as wild predatory mammals. Dogs in the region have two types of dirofilaria cardionematoda Dirofilaria immitis and subcutaneous Dirofilaria repens. The highest extensity of invasion (EI) Dirofilaria immitis autopsy from 71.4 to 87.5% have dogs in overflow land, foothill and mountain zones of the region and the smallest from 33.3 to 40.0% in the lowlands. According to the results of a parasitological study of blood, the highest and lowest EI of Dirofilaria immitis was also found in these zones and amounted to 28.7 to 30.6% and 17.7 to 18.8%, respectively. The average EI of dirofilaria Dirofilaria immitis depends on the area of the study region, on the breed and the method of detention and use of dogs. According to the results of the autopsy, the average EI for the study period was 58.8%, according to the results of the Knott blood test – 26.0%, and the test system – 28.8%. The largest number of dogs infected with dirofilariasis were between the ages of 4 and 9 years. The most infected are short-haired breeds, yard dogs, guard dogs, hunting dogs, and service dogs. Sexual dependence is not established.

Pathology associated with three new Clinostomum metacercariae from Argentina with morphological and DNA barcode identification

In the Laboratory of Parasites of Fishes, Crustaceans and Mollusks (CEPAVE), we undertook a parasitological study on three species of fish from the Espinal and Esteros del Iberá ecoregions of Argentina. Clinostomid metacercariae were found parasitizing Characidium rachovii, Crenicichla vittata and Gymnogeophagus balzanii. In this study, we analysed the damage that these parasites inflict on their hosts through the evaluation of histological sections. In addition, Clinostomum metacercariae were identified using morphological characters and DNA barcoding. In the pathological analysis, we observed that muscle tissue was the most affected. The inflammatory response showed vascular congestion areas and infiltration of numerous inflammatory cells, mainly lymphocytes. The molecular and morphological approach supports the presence of three new lineages of clinostomid metacercariae in Argentina. This could lead to the discovery of a high number of lineages or species of Clinostomum from South America.

Comparison of Helminth Infection among the Native Populations of the Arctic and Subarctic Areas in Western Siberia Throughout History: Parasitological Researches on Contemporary and the Archaeological Resources

The aim of this parasitological study is examining contemporary (the late 20th century) specimens of the arctic or subarctic areas in Western Siberia and comparing them with the information acquired from archaeological samples from the same area. In the contemporary specimens, we observed the parasite eggs of 3 different species: <i>Opisthochis</i> <i>felineus</i>, <i>Ascaris</i> <i>lumbricoides</i>, and <i>Enterobius</i> <i>vermicularis</i>. Meanwhile, in archaeoparasitological results of Vesakoyakha, Kikki-Akki, and Nyamboyto I burial grounds, the eggs of <i>Diphyllobothrium</i> and <i>Taenia</i> spp. were found while no nematode (soil-transmitted) eggs were observed in the same samples. In this study, we concluded helminth infection pattern among the arctic and subarctic peoples of Western Siberia throughout history as follows: the raw fish-eating tradition did not undergo radical change in the area at least since the 18th century; and <i>A</i>. <i>lumbricoides</i> or <i>E</i>. <i>vermicularis</i> did not infect the inhabitants of this area before 20th century. With respect to the Western Siberia, we caught glimpse of the parasite infection pattern prevalent therein via investigations on contemporary and archaeoparasitological specimens.

Trials by application the drugs «Prazitsid®-suspension Plus» and «Dirofen® tablets» at hemorrhagic diarrhea in cats with giardiasis

The results of a pilot study of anthelmintics drugs ― «Prazicide®-suspension Plus» and «Dirofen® tablets» in the treatment of diarrhea in cats with giardiosis are given. The studied drugs contain febantel, which has an anti-Giardia effect, it makes possible to recommend them to animals in cases of suspected protozoa invasion. The drugs are developed and introduced into clinical practice as anthelmintics of a wide spectrum of action for the regular deworming of small pets. Data of clinical and laboratory allow us to expand the range of application of these drugs in cats, in particular, to recommend them to animals with gastrointestinal disorders, manifesting symptoms of classical acute diarrhea. The clinical efficacy criteria were: repeated parasitological study of feces, as well as negative samples of laboratory express-tests (IHA, ELISA or PCR) taking into account the dynamics of elimination of diarrhea symptoms and flatulence in cats. A coproscopic analysis was performed by classical parasitological methods ― by microscopy of native of feces and / or supernatant after preparation of the sample according to the Fulleborn method (1920). The results of our studies showed the clinical efficacy in the treatment of feline giardiosis of «Prazicide®-suspension Plus» and «Dirofen® tablets» (developed by API-SAN LLC) with a triple use in a therapeutic dose, according to the instructions.