scholarly journals Effects of preoperative pelvic irradiation on colonic anastomosis healing: an experimental study in rats

2008 ◽  
Vol 23 (suppl 1) ◽  
pp. 24-30 ◽  
Author(s):  
Alexandre Franca ◽  
Fernando Silva Ramalho ◽  
Leandra Naira Zambelli Ramalho ◽  
José Joaquim Ribeiro da Rocha ◽  
Omar Féres
Keyword(s):  
Colonic Anastomosis ◽  
Intestinal Anastomosis ◽  
Healing Process ◽  
Colorectal Anastomosis ◽  
Time Interval ◽  
Preoperative Irradiation ◽  
Vascular Endothelial ◽  
Healing Tissue ◽  
Endothelial Growth Factor ◽  
Anastomosis Healing

PURPOSE: Colorectal anastomosis is a constant worry-issue among surgeons because of high rates of complications, specially the dehiscence. The preoperative irradiation on cancer surgeries might interfere in the healing process, leading to an unfavorable outcome. METHODS: In the present study, two groups of rats were irradiated previously to a colorectal anastomosis surgery, with intervals of 4 and 8 weeks between the procedures. Seven days after the surgery, healing process was evaluated for dehiscence presence and histologic inflammatory characteristics. Also, levels of hydroxyproline, metalloproteinases and vascular endothelial growth factor were measured. RESULTS: Our results showed a higher incidence of dehiscences on the animals submitted to irradiation, compared to controls, with a reduced inflammatory activity in the healing tissue. DISCUSSION: Comparing both irradiated groups, those irradiated 8 weeks before surgery showed higher levels of hydroxyproline and metalloproteinases, indicating higher efficiency of the healing process. In conclusion, preoperative irradiation interferes with intestinal anastomosis healing and a larger time interval between both procedures is safer in terms of the healing quality.

scholarly journals Effect of thalidomide on the healing of colonic anastomosis, in rats

2008 ◽  
Vol 23 (suppl 1) ◽  
pp. 17-23 ◽  
Author(s):  
Samuel Gama Veneziano ◽  
Leandra Naira Zambelli Ramalho ◽  
Fernando Silva Ramalho ◽  
Antônio Dorival Campos ◽  
José Joaquim Ribeiro da Rocha ◽  
...  
Keyword(s):  
Saline Solution ◽  
Colonic Anastomosis ◽  
Healing Process ◽  
Immunohistochemical Analysis ◽  
Oral Route ◽  
Male Rats ◽  
Vascular Endothelial ◽  
Significant Difference ◽  
Endothelial Growth Factor

PURPOSE: Thalidomide, because of its anti-inflammatory properties, as re-emerged as an option for the treatment of Crohn's disease refractory to standard therapy. We studied the effect of thalidomide on the healing of colonic anastomosis. METHODS: Sixty male rats (Rattus norvegicus), were divided into 3 groups of 20 animals each, respectively receiving 0.5 or 1.0 mg/kg thalidomide by the oral route for 7 days, or saline solution (control). All animals were submitted to continuous end-to-end anastomosis with 6-0 Prolene sutures. After sacrifice the anastomoses were analyzed macroscopically and submitted to determination of hydroxyproline, to histology and to immunohistochemistry for metalloproteinase 1, metalloproteinase 1 inhibitor and vascular endothelial growth factor (VEGF). RESULTS: Statistical analysis of the data showed no significant difference in macroscopic aspect or hydroxyproline determination (p= 0.5403). In the immunohistochemical analysis, the following p values were obtained: p = 0.5817 for VEGF, p = 0.1854 for metalloproteinase 1, and p = 0.0023 for metalloproteinase 1 inhibitor, with this last value being considered statistically significant. CONCLUSION: We conclude that thalidomide influenced collagen maturation. There was a stronger action of metalloproteinases, possibly indicating a negative tendency for the healing process.

Fracture Healing and Biomarker Expression in a Diabetic Zucker Rat Model

2014 ◽  
Vol 104 (5) ◽  
pp. 428-433 ◽  
Author(s):  
Javier La Fontaine ◽  
Nathan A. Hunt ◽  
Stacey Curry ◽  
Tyler Kearney ◽  
Daniel Jupiter ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fracture Healing ◽  
Bone Healing ◽  
Rat Model ◽  
Healing Process ◽  
Diabetic Rats ◽  
Vascular Endothelial ◽  
Delayed Healing ◽  
Endothelial Growth Factor

Background Persons with diabetes have a higher incidence of fractures compared with persons without diabetes. However, there is little published information concerning the deleterious effect of late-stage diabetes on fracture healing. There are no studies using animal models that evaluate the effect of advanced diabetes on fracture healing. The purpose of our study was to evaluate cytokine expression, specifically macrophage inflammatory protein 1 (MIP-1) and vascular endothelial growth factor, in fracture healing in a type 2 diabetes rat model. Methods We evaluated biomarker expression after femur fracture using a rat model. The two groups consisted of 24 Zucker diabetic rats (study group) and 12 Zucker lean rats (control group). An independent reviewer was used to assess delayed union. We evaluated serum samples 2, 4, 7, and 14 days after surgery for MIP-1, vascular endothelial growth factor, leptin, and other cytokine levels. Results At 3 weeks, Kaplan-Meier estimates showed that 45.8% of femur fractures in Zucker diabetic rats had healed, whereas 81.8% of those in Zucker lean rats had healed (P = .02). A logistic regression model to predict fast healing that included the three cytokines and diabetes status showed that the only factor achieving significance was MIP-1α. Vascular endothelial growth factor was the only biomarker to show significance compared with delayed healing. Conclusions These results confirm significant differences in biomarker expression between diabetic and nondiabetic rats during bone healing. The key factors for bone healing may appear early in the healing process, whereas differences in diabetes versus nondiabetes are seen later in the healing process. Increased levels of MIP-1α were associated with the likelihood of delayed healing.

scholarly journals The Determination of Appendicitis from Folate Acid and Vascular Endothelial Growth Factor Level in Animal Model: A Review

2021 ◽  
Vol 9 (F) ◽  
pp. 395-398
Author(s):  
Erjan Fikri ◽  
Putri Chairani Eyanoer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Animal Model ◽  
Growth Factor ◽  
Healing Process ◽  
Vascular Endothelial ◽  
Tissue Healing ◽  
Study Results ◽  
Abdominal Emergencies ◽  
Endothelial Growth Factor

BACKGROUND: Appendicitis is one of the most common abdominal emergencies that require surgery in children. The morbidity and mortality rate in appendicitis is greatly affected by rupture or leakage of the appendix. In establishing the diagnosis of appendicitis, several modalities are acknowledged, namely, pediatric appendicitis score and ultrasound. Pathologically, severity and complications of appendicitis occur related to good vascularization and tissue healing process through process of angiogenesis influenced by folic acid (FA) and vascular endothelial growth factor (VEGF). This is clinically important as currently non-operative and non-invasive therapies were developing in stratification of mild appendicitis. AIM: This study aimed to review the determination of appendicitis from FA and VEGF levels in animal model. METHODS: This study was conducted in accordance with the PRISMA guidelines for reporting systematic reviews. Articles were reviewed for relation of FA and VEGF in determining appendicitis in PubMed and Science Direct. Articles on experimental animal model published from 1990 to 2020 were included, while articles in English were excluded from the study. RESULTS: The articles we reviewed conduct an assessment of appendicitis by FA and VEGF level in animal model. Three articles were reviewed ranging from 1990 to 2020. One article presented a significant association of decreased level of FA in determining appendicitis while the other two mentioned trends of decreased level of FA and VEGF in appendicitis without statistically significance. CONCLUSION: Reduction of folate acid levels could be a critical prescient factor for the weight of muddled an appendicitis in animal model, however, there was a pattern demonstrating low estimation of VEGF as an indicator of appendicitis and convoluted appendix with no measurable note worthiness appeared.

scholarly journals The Effect of TISSEEL® on Colorectal Anastomosis Healing Process in a Diabetic Animal Experimental Model

In Vivo ◽  
2020 ◽  
Vol 34 (2) ◽  
pp. 659-665
Author(s):  
KONSTANTINOS STERGIOS ◽  
MAXIMOS FROUNTZAS ◽  
VASILIOS PERGIALIOTIS ◽  
LASKARINA MARIA KOROU ◽  
KONSTANTINOS KONTZOGLOU ◽  
...  
Keyword(s):  
Experimental Model ◽  
Healing Process ◽  
Colorectal Anastomosis ◽  
Diabetic Animal ◽  
Anastomosis Healing ◽  
Animal Experimental Model

scholarly journals Double Perforation after Colorectal Cancer Surgery During Bevacizumab Treatment

2020 ◽  
Vol 27 (4) ◽  
pp. 309-312
Author(s):  
Lucian Sorin ANDREI ◽  
Radu Sorin POPISTEANU ◽  
Adriana ANDREI ◽  
Alexandru MICU ◽  
Ioana DINU
Keyword(s):  
Colorectal Cancer ◽  
Monoclonal Antibody ◽  
Vascular Endothelial Growth Factor ◽  
Small Intestine ◽  
Colorectal Anastomosis ◽  
Vascular Endothelial ◽  
Colorectal Cancer Surgery ◽  
Bevacizumab Treatment ◽  
Endothelial Growth Factor ◽  
Metastasized Colorectal Cancer

Bevacizumab is a monoclonal antibody which has shown promising results in the treatment of varied malignant pathology, including metastasized colorectal cancer. It acts by inhibiting VEGF (vascular endothelial growth factor), and one of its most cited complications is intestinal perforation, by mechanisms which are not yet fully understood. We present the case of a 68 year old patient, operated for metastasized colorectal cancer, which underwent chemotherapy with Bevacizumab, and shortly after initiating therapy developed fi stula of low colorectal anastomosis, followed by ischemic perforation of the small intestine.

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