scholarly journals Regulation of in vitro somatic embryogenesis with emphasis on to the role of endogenous hormones

2001 ◽  
Vol 13 (2) ◽  
pp. 196-223 ◽  
Author(s):  
VÍCTOR M. JIMÉNEZ
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Growth Regulators ◽  
Culture Medium ◽  
Endogenous Hormones ◽  
Tissue Cultures ◽  
Factors Associated ◽  
General Aspects

Different aspects of the in vitro somatic embryogenesis regulation are reviewed in this paper.work. A description of g General aspects, such as terminology, uses, stages of development and factors associated with the somatic embryogenesis, are described. is carried out. Although a brief description ofn the effects of the addition of different plant growth regulators to the culture medium wasis given, the article is centereds itself on the effect that the endogenous hormone concentrations in the initial explants and in the tissue cultures derived from them could play oin the induction and expression of somatic embryogenesis. It is significant that few to emphasize the low amount of systematic studies have been conducted, in this subject, in which different species and hormone groups were compared in cultures with and without embryogenic capacity. Moreover, the lack of correlation between the results presented in different studies the distinct works indicates that the hormone content of the cultures is not the only factor involved.

scholarly journals In vitro propagation of Althaea officinalis : the role of plant growth regulators in morphogenesis

2017 ◽  
Vol 98 (3) ◽  
pp. 167-173
Author(s):  
A. Mujib ◽  
Tanu Pipal ◽  
Muzamil Ali ◽  
Dipti Tonk ◽  
Nadia Zafar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Althaea Officinalis

scholarly journals Role of plant growth regulators in preservation of Pyrus germplasm in vitro

2011 ◽  
Vol 10 (64) ◽  
pp. 14029-14037
Author(s):  
Ahmed Maqsood ◽  
Akbar Anjum Muhammad ◽  
Jamil Ahmed Mohammad ◽  
Mustafa Sajid Ghulam ◽  
Yoqub Abid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators

scholarly journals Evaluation of In Vitro Shoot Elongation and Rooting of Date Palm, and Determination of Physiological Characteristics of Regenerated Plantlets

2019 ◽  
Vol 11 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Reda MEZIANI ◽  
Mouaad Amine MAZRI ◽  
Mahassine ARHAZZAL ◽  
Ilham BELKOURA ◽  
Chakib ALEM ◽  
...  
Keyword(s):  
Survival Rate ◽  
Stomatal Conductance ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Date Palm ◽  
Shoot Elongation ◽  
Physiological Characteristics

The effects of various culture conditions on shoot elongation, rooting and plantlet acclimatization were tested. Adventitious shoots obtained through direct organogenesis of date palm (Phoenix dactylifera L.) cv. ‘Mejhoul’ were used as explants. The effects of culture medium texture, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol, L-glutamine, and carbon source on in vitro plantlet quality and subsequent acclimatization were evaluated. The most effective culture medium was the semi-solid and half-strength Murashige and Skoog medium without plant growth regulators, supplemented with 30 g L-1 sucrose. After 3 months of culture on this medium, the average shoot length was 13.6 cm, the average number of adventitious roots per shoot was 3.6, and the average root length was 3.85 cm. The survival rate of these plantlets in acclimatization was 90%. On the other hand, liquid medium, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol and L-glutamine did not increase the survival rate during acclimatization. Along with these experiments, some physiological characteristics of the plantlets obtained in vitro were also determined. Chlorophyll content and fluorescence, foliar surface and stomatal conductance were measured after 3 months of culture in each medium. The ranges were as follows: Chlorophyll content, 11.7-31.8 CCI; chlorophyll fluorescence, 0.633-0.795; foliar surface, 7.35-13.29 cm2; and stomatal conductance, 10.3-36.0 mmol m-2 s-1. Interestingly, positive correlations between the physiological characteristics of the plantlets and their survival percentage in the glasshouse were revealed. The findings of this investigation will be valuable for large-scale and cost-saving production of date palm cv. ‘Mejhoul’ plants.

scholarly journals Somatic embryogenesis from Hoan ngoc(Pseuderanthemum palatiferum (Ness) Radlk) leaf callus

2014 ◽  
Vol 17 (2) ◽  
pp. 100-107
Author(s):  
Nhung Thi Tuyet Tran ◽  
Hoang Ngo Phan ◽  
Sanh Du Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Biological Activity ◽  
Plant Growth ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Leaf Tissue ◽  
Ms Medium ◽  
The Mean ◽  
Endogenous Plant

Pseuderanthemum palatiferum contains many compounds which have pharmaceutical and medicinal values. In this study, the in vitro somatic embryogenesis of callus formed from Pseuderanthemum palatiferum’s leaves was performed and analyzed. The calli were induced in three weeks in MS medium with 1 mg.l-1 or 2 mg.l-1 of 2,4-D. The somatic embryos were developed as follows: the globular embryos formed after 9 days, the heart embryos formed after 13 days, the torpedo embryos formed after 15 days and the cotyledonary embryos formed after 17 days. The mean number of obtained embryos was 28.5 embryos/cm2 of leaf tissue. The intensity of respiration and biological activity of endogenous plant growth regulators of leaf tissue during culture were recorded.

scholarly journals Apprehending the potential of BABY BOOM transcription factors to mitigate cotton regeneration and transformation

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Caner YAVUZ ◽  
Shakhnozakhan TILLABOEVA ◽  
Allah BAKHSH
Keyword(s):  
Somatic Embryogenesis ◽  
Transcription Factors ◽  
Growth Regulators ◽  
Baby Boom ◽  
In Vitro Regeneration ◽  
Signal Transduction Pathway ◽  
Breeding Programmes ◽  
Transformation Problems

Abstract Since the advent of transgenic technology, the incorporation of gene(s) encoding traits of economic importance in cotton is being practiced worldwide. However, factors like recalcitrant nature of cotton cultivars, in vitro regeneration via tissue culture (especially via somatic embryogenesis), genotype dependency, long and toilsome protocols impede the pace of development of transgenic cotton. Besides that, types and age of explants, media composition, plant growth regulators and other environmental factors affect in vitro cotton regeneration significantly. The studies of genetic control of in vitro regeneration in plants have elucidated the role of certain transcription factor genes that are induced and expressed during somatic embryogenesis. Among these transcription factors, BABY BOOM (BBM) plays a very important role in signal transduction pathway, leading to cell differentiation and somatic embryos formation. The role of BBM has been established in plant cell proliferation, growth and development even without exogenous growth regulators. This review intends to provide an informative summary of regeneration and transformation problems in cotton and the latest developments in utilization of BBM transcription factors in cotton. We believe that the use of BBM will not only ease cotton genetic improvement but will also accelerate cotton breeding programmes.

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