scholarly journals An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis

2009 ◽  
Vol 39 (5) ◽  
pp. 1577-1580 ◽  
Author(s):  
Tessália Diniz Luerce ◽  
Vanessa Galli ◽  
Gustavo Maia Cerqueira ◽  
Simone Simionatto ◽  
Odir Antônio Dellagostin
Keyword(s):  
Restriction Analysis ◽  
Pcr Amplification ◽  
Previous Treatment ◽  
Enzyme Electrophoresis ◽  
Improved Method ◽  
Stress Syndrome ◽  
Gene Coding ◽  
Ethidium Bromide Staining ◽  
Porcine Stress Syndrome ◽  
Ryanodine Receptor 1

A mutation in the gene coding for the ryanodine receptor 1 (RYR1), also known as halothane (hal) gene or swine stress gene, is associated to the porcine stress syndrome (PSS). Detection of the mutation is normally accomplished by PCR amplification of an 81bp fragment of the hal gene, followed by digestion with the HhaI restriction endonuclease. Wild-type allele (N) is cut in two fragments, whereas the mutant allele (n) is not digested by the restriction enzyme. Electrophoresis of the digested DNA on agarose gel and ethidium bromide staining allows the reading of the result. The correct interpretation is difficult due to the small size of the DNA fragments. In this study we designed a new set of primers for amplification of a 144bp fragment that facilitates the reading of the result. In addition, we optimized the PCR reaction to allow amplification from a single hair bulb, added directly into the PCR mix without previous treatment. This improved method was used to genotype 165 sows and boars used in a breeding program. Forty-nine percent of the animals had the NN genotype, whereas 50% were Nn and only 1% was nn.

scholarly journals Porcine stress syndrome (PSS) and ryanodine receptor 1 (RYR1) gene mutation in European wild pig (Sus scrota ferus)

2005 ◽  
Vol 55 (2-3) ◽  
pp. 251-255 ◽  
Author(s):  
Jovanovic Slobodan ◽  
Trailovic Ruzica ◽  
Savic Mila ◽  
Sarac M.
Keyword(s):  
Gene Mutation ◽  
Ryanodine Receptor ◽  
Ryr1 Gene ◽  
Stress Syndrome ◽  
Porcine Stress Syndrome ◽  
Ryanodine Receptor 1 ◽  
Wild Pig

PCR Amplification and Bioinformatics of a Novel Gene Coding Hydroxylamine Oxidoreductase*

2010 ◽  
Vol 2009 (1) ◽  
pp. 100-105
Author(s):  
Zuguo ZHAO ◽  
Qingxin KONG ◽  
Jingfeng WANG ◽  
Min JIN ◽  
Xinwei WANG ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Hydroxylamine Oxidoreductase ◽  
Novel Gene ◽  
Gene Coding

scholarly journals A defect in dystrophin causes a novel porcine stress syndrome

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 233 ◽  
Author(s):  
Dan J Nonneman ◽  
Tami Brown-Brandl ◽  
Shuna A Jones ◽  
Ralph T Wiedmann ◽  
Gary A Rohrer
Keyword(s):  
Stress Syndrome ◽  
Porcine Stress Syndrome

scholarly journals Associations of biochemical changes and maternal traits with mutation 1843 (C>T) in the RYR1 gene as a common cause for porcine stress syndrome

2016 ◽  
Vol 19 (2) ◽  
pp. 75-80 ◽  
Author(s):  
ZT Popovski ◽  
B Tanaskovska ◽  
E Miskoska-Milevska ◽  
S Andonov ◽  
S Domazetovska
Keyword(s):  
Fragment Length Polymorphism ◽  
Protein Product ◽  
Biochemical Changes ◽  
Chain Reaction ◽  
Maternal Characteristics ◽  
Stress Syndrome ◽  
Pcr Rflp ◽  
Porcine Stress Syndrome ◽  
Heterozygous Carriers ◽  
Polymerase Chain

AbstractStress syndrome is usually caused by a mutation in theryanodine receptorgene(ryr1) and it is widely studied in humans and swine populations. The protein product of this gene plays a crucial role in the regulation of calcium transport in muscle cells. A G>T mutation in the humanryr1gene, which results in the replacement of a conserved arginine at position 614 where a leucine occurs at the same position as the previously identified Arg→Cys mutation reported in all cases of porcine stress syndrome (PSS). Porcine stress syndrome affects biochemical pathways in stress-susceptible individuals during a stress episode and some biochemical parameters that were used as markers for diagnostic purposes. Also, PSS has remarkable influence on the maternal characteristics of sows. This study dealt with different genotypes for PSS and its association with possible biochemical changes and maternal traits of sows. Seventy-three reproductive sows genotyped for PSS by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were included in this survey. Sixty of them were stress-free (NN), 11 were heterozygous carriers (Nn) and two animals were homozygous (nn) for the 1843 (C>T) mutation. Significant differences in non stress induced animals with different PSS genotypes were found in the values of creatine phoshokinase (CPK), lactate dehydrogenase (LDH), alkaline phosphatase (AP) and aspartate aminotransferase (AST). Regarding the maternal traits, our study showed that stress susceptible animals (nn) have an increased number of stillborn piglets and a reduced number of newborn piglets compared with heterozygous and normal animals.

scholarly journals Relationship between the Porcine Stress Syndrome gene and pork quality traits of F2 pigs resulting from divergent crosses

2005 ◽  
Vol 28 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Guilherme de Oliveira Band ◽  
Simone Eliza Facioni Guimarães ◽  
Paulo Sávio Lopes ◽  
Alex Sandro Schierholt ◽  
Kleibe Moraes Silva ◽  
...  
Keyword(s):  
Pork Quality ◽  
Quality Traits ◽  
Stress Syndrome ◽  
Porcine Stress Syndrome

scholarly journals Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

2000 ◽  
Vol 66 (10) ◽  
pp. 4340-4344 ◽  
Author(s):  
T. Deak ◽  
J. Chen ◽  
L. R. Beuchat
Keyword(s):  
Yarrowia Lipolytica ◽  
Rapd Analysis ◽  
Restriction Analysis ◽  
Pcr Amplification ◽  
Yeast Cells ◽  
Chromosomal Dna ◽  
Intergenic Sequences ◽  
Species Specific ◽  
Candida Zeylanoides ◽  
Its Pcr

ABSTRACT Yeast isolates from raw and processed poultry products were characterized using PCR amplification of the internally transcribed spacer (ITS) 5.8S ribosomal DNA region (ITS-PCR), restriction analysis of amplified products, randomly amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE). ITS-PCR resulted in single fragments of 350 and 650 bp, respectively, from eight strains of Yarrowia lipolytica and seven strains of Candida zeylanoides. Digestion of amplicons with HinfI andHaeIII produced two fragments of 200 and 150 bp fromY. lipolytica and three fragments of 350, 150, and 100 bp from C. zeylanoides, respectively. Although these fragments showed species-specific patterns and confirmed species identification, characterization did not enable intraspecies typing. Contour-clamped heterogeneous electric field PFGE separated chromosomal DNA of Y. lipolytica into three to five bands, most larger than 2 Mbp, whereas six to eight bands in the range of 750 to 2,200 bp were obtained from C. zeylanoides. Karyotypes of both yeasts showed different polymorphic patterns among strains. RAPD analysis, using enterobacterial repetitive intergenic sequences as primers, discriminated between strains within the same species. Cluster analysis of patterns formed groups that correlated with the source of isolation. For ITS-PCR, extraction of DNA by boiling yeast cells was successfully used.

Effects of preslaughter management on the quality of carcasses from porcine stress syndrome heterozygous market hogs.

1998 ◽  
Vol 76 (9) ◽  
pp. 2435 ◽  
Author(s):  
K J Stalder ◽  
J Maya ◽  
L L Christian ◽  
S J Moeller ◽  
K J Prusa
Keyword(s):  
Stress Syndrome ◽  
Porcine Stress Syndrome

scholarly journals Molecular Typing of Borrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

1999 ◽  
Vol 12 (4) ◽  
pp. 633-653 ◽  
Author(s):  
Guiqing Wang ◽  
Alje P. van Dam ◽  
Ira Schwartz ◽  
Jacob Dankert
Keyword(s):  
Restriction Analysis ◽  
Clinical Manifestations ◽  
Taxonomic Status ◽  
Sensu Stricto ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Clinical Implications ◽  
Rrna Gene ◽  
Enzyme Electrophoresis ◽  
Borrelia Lusitaniae ◽  
Dna Reassociation

SUMMARY Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the LB-related spirochetes and their potential association with distinct clinical syndromes. These methods include serotyping, multilocus enzyme electrophoresis, DNA-DNA reassociation analysis, rRNA gene restriction analysis (ribotyping), pulsed-field gel electrophoresis, plasmid fingerprinting, randomly amplified polymorphic DNA fingerprinting analysis, species-specific PCR and PCR-based restriction fragment length polymorphism (RFLP) analysis, and sequence analysis of 16S rRNA and other conserved genes. On the basis of DNA-DNA reassociation analysis, 10 different Borrelia species have been described within the B. burgdorferi sensu lato complex: B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, Borrelia japonica, Borrelia andersonii, Borrelia valaisiana, Borrelia lusitaniae, Borrelia tanukii, Borrelia turdi, and Borrelia bissettii sp. nov. To date, only B. burgdorferi sensu stricto, B. garinii, and B. afzelii are well known to be responsible for causing human disease. Different Borrelia species have been associated with distinct clinical manifestations of LB. In addition, Borrelia species are differentially distributed worldwide and may be maintained through different transmission cycles in nature. In this paper, the molecular methods used for typing of B. burgdorferi sensu lato are reviewed. The current taxonomic status of B. burgdorferi sensu lato and its epidemiological and clinical implications, especiallly correlation between the variable clinical presentations and the infecting Borrelia species, are discussed in detail.

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