scholarly journals Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection

2003 ◽  
Vol 40 (5) ◽  
Author(s):  
Marcelo Fernandes Camargos ◽  
Daniel Stancek ◽  
Leandro Moreira Lessa ◽  
Jenner Karlisson Pimenta Reis ◽  
Maurílio Andrade Rocha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Virus Infection ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Leukemia Virus Infection ◽  
Chain Reaction ◽  
Agar Gel ◽  
Immunodiffusion Test ◽  
Polymerase Chain

scholarly journals Detection of Bovine Leukemia Virus by in Situ Polymerase Chain Reaction in Tissues from a Heifer Diagnosed with Sporadic Thymic Lymphosarcoma

2005 ◽  
Vol 17 (2) ◽  
pp. 190-194 ◽  
Author(s):  
Robert B. Duncan ◽  
William K. Scarratt ◽  
Gertrude C. Buehring
Keyword(s):  
Polymerase Chain Reaction ◽  
Epithelial Cells ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Sudden Onset ◽  
Chain Reaction ◽  
Liver And Kidney ◽  
Cellular Tropism ◽  
Polymerase Chain

An 18-month-old bovine heifer was presented for clinical evaluation after a sudden onset of ventral edema. Clinical and pathological evaluations were consistent with thymic lymphosarcoma, a sporadic form of lymphosarcoma in cattle, which is not generally considered to be associated with bovine leukemia virus (BLV). This heifer was seropositive for BLV at 6 and 18 months of age. Tissues obtained at necropsy were evaluated using in situ polymerase chain reaction. The BLV proviral DNA was detected in lymphocytes of the thymus as well as in epithelial cells of the liver and kidney. This report presents evidence that thymic lymphosarcomas can be associated with BLV infection and that BLV may have a broader cellular tropism than was supposed previously.

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