scholarly journals Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

2006 ◽  
Vol 49 (3) ◽  
pp. 411-419 ◽  
Author(s):  
Gildo Almeida da Silva ◽  
Erik Amazonas de Almeida
Keyword(s):  
Pseudomonas Fluorescens ◽  
Pigment Production ◽  
Siderophore Production ◽  
Mineral Salts ◽  
Fluorescent Pigment ◽  
Green Fluorescent

A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

The green fluorescent pigment of pseudomonas fluorescens

1938 ◽  
Vol 57 (12) ◽  
pp. 1397-1404 ◽  
Author(s):  
A. Turfreijer ◽  
J. P. Wibaut ◽  
T. Y. Kingma Boltjes
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fluorescent Pigment ◽  
Green Fluorescent

scholarly journals Genetic Analysis of Fluorescent Pigment Production in Pseudomonas syringae pv. syringae

Microbiology ◽  
1984 ◽  
Vol 130 (6) ◽  
pp. 1507-1515 ◽  
Author(s):  
J. E. Loper ◽  
C. S. Orser ◽  
N. J. Panopoulos ◽  
M. N. Schroth
Keyword(s):  
Genetic Analysis ◽  
Pseudomonas Syringae ◽  
Pigment Production ◽  
Fluorescent Pigment

Strain Diversity of Pseudomonas fluorescens Group with Potential Blue Pigment Phenotype Isolated from Dairy Products

2016 ◽  
Vol 79 (8) ◽  
pp. 1430-1435 ◽  
Author(s):  
MARGHERITA CHIERICI ◽  
CLAUDIA PICOZZI ◽  
MARISA GRAZIA LA SPINA ◽  
CARLA ORSI ◽  
ILEANA VIGENTINI ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Sodium Succinate ◽  
16S Rrna Gene Sequencing ◽  
Pigment Production ◽  
Blue Pigment ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Strain Diversity ◽  
Blue Discoloration ◽  
Rrna Gene Sequencing

ABSTRACT The blue discoloration in Mozzarella cheese comes from bacterial spoilage due to contamination with Pseudomonas. Fourteen Pseudomonas fluorescens strains from international collections and 55 new isolates of dominant bacterial populations from spoiled fresh cheese samples were examined to assess genotypic and phenotypic strain diversity. Isolates were identified by 16S rRNA gene sequencing and tested for the production of the blue pigment at various temperatures on Mascarpone agar and in Mozzarella preserving fluid (the salty water in which the cheese is conserved, which becomes enriched by cheese minerals and peptides during storage). Pulsed-field gel electrophoresis analysis after treatment with the endonuclease SpeI separated the isolates into 42 genotypes at a similarity level of 80%. Based on the pulsotype clustering, 12 representative strains producing the blue discoloration were chosen for the multilocus sequence typing targeting the gyrB, glnS, ileS, nuoD, recA, rpoB, and rpoD genes. Four new sequence typing profiles were discovered, and the concatenated sequences of the investigated loci grouped the tested strains into the so-called “blue branch” of the P. fluorescens phylogenetic tree, confirming the linkage between pigment production and a specific genomic cluster. Growth temperature affected pigment production; the blue discoloration appeared at 4 and 14°C but not at 30°C. Similarly, the carbon source influenced the phenomenon; the blue phenotype was generated in the presence of glucose but not in the presence of galactose, sodium succinate, sodium citrate, or sodium lactate.

scholarly journals Siderophore cooperation of the bacterium Pseudomonas fluorescens in soil

2015 ◽  
Vol 11 (2) ◽  
pp. 20140934 ◽  
Author(s):  
Adela M. Luján ◽  
Pedro Gómez ◽  
Angus Buckling
Keyword(s):  
Spatial Structure ◽  
Social Interactions ◽  
Pseudomonas Fluorescens ◽  
Physical Environment ◽  
Natural Populations ◽  
Siderophore Production ◽  
Rare Advantage ◽  
Social Trait ◽  
Better Than

While social interactions play an important role for the evolution of bacterial siderophore production in vitro , the extent to which siderophore production is a social trait in natural populations is less clear. Here, we demonstrate that siderophores act as public goods in a natural physical environment of Pseudomonas fluorescens : soil-based compost. We show that monocultures of siderophore producers grow better than non-producers in soil, but non-producers can exploit others' siderophores, as shown by non-producers' ability to invade populations of producers when rare. Despite this rare advantage, non-producers were unable to outcompete producers, suggesting that producers and non-producers may stably coexist in soil. Such coexistence is predicted to arise from the spatial structure associated with soil, and this is supported by increased fitness of non-producers when grown in a shaken soil–water mix. Our results suggest that both producers and non-producers should be observed in soil, as has been observed in marine environments and in clinical populations.

scholarly journals Identification of Pseudomonas fluorescens using different biochemical tests

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Bikram Nepali ◽  
Sabin Bhattarai ◽  
Jiban Shrestha
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fungal Pathogens ◽  
Rice Seed ◽  
Fungal Metabolites ◽  
Maize Seed ◽  
Biochemical Tests ◽  
Pathology Laboratory ◽  
Fluorescent Pigment ◽  
Oxidase Test ◽  
Anti Fungal

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.

Influence of sulfite on growth, slime, and fluorescent pigment formation by Pseudomonas aeruginosa

1973 ◽  
Vol 19 (4) ◽  
pp. 505-511 ◽  
Author(s):  
Samuel A. Palumbo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Active Agent ◽  
Medium Ph ◽  
Pigment Formation ◽  
Ph Adjustment ◽  
Fluorescent Pigment ◽  
Cultural Conditions ◽  
Green Fluorescent

Substitution of sulfite for sulfate in the defined pyocyanine medium of Frank and DeMoss 1959 allowed formation of fluorescent pigments and slime by Pseudomonas aeruginosa NRRL B-4014. This formation of fluorescent pigments was both pH and iron dependent. The unadjusted medium (pH 8.25) containing sulfite and Fe3+ allowed both growth and fluorescent pigment formation. Growth and fluorescent pigment formation were observed from a pH of 9.0 down to a pH of 7.5. At pH 7.5, the concentration of HSO3− is 6 × 10−4 M, and this ion appears to be the active agent in inhibiting growth below pH 7.5. When the medium was adjusted to pH 7.0, neither fluorescent pigment formation nor growth was observed. The acid used for pH adjustment did not influence the minimum pH for growth. In the presence of small amounts of iron (ca. 1 × 10−6 M Fe3+), the organism shifted from a blue to a yellow-green fluorescent pigment. Cultural conditions such as temperature and nutrients which supported growth also supported slime formation as well as fluorescent pigment formation.

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