Pupal Cuticle Protein Is Abundant During Early Adult Diapause in the MosquitoCulex pipiens

2009 ◽  
Vol 46 (6) ◽  
pp. 1382-1386 ◽  
Author(s):  
Aiqing Li ◽  
David L. Denlinger
Keyword(s):  
Adult Diapause ◽  
Cuticle Protein ◽  
Pupal Cuticle

scholarly journals The pupal cuticle of Drosophila: biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone.

1985 ◽  
Vol 101 (1) ◽  
pp. 189-200 ◽  
Author(s):  
J Doctor ◽  
D Fristrom ◽  
J W Fristrom
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ultrastructural Changes ◽  
Low Molecular Weight ◽  
Before And After ◽  
Cuticle Protein ◽  
Cuticle Proteins ◽  
Pupal Cuticle

We investigated the synthesis and localization of Drosophila pupal cuticle proteins by immunochemical techniques using both a complex antiserum and monoclonal antibodies. A set of low molecular weight (15,000-25,000) pupal cuticle proteins are synthesized by the imaginal disk epithelium before pupation. After pupation, synthesis of the low molecular weight proteins ceases and a set of unrelated high molecular weight proteins (40,000-82,000) are synthesized and incorporated into the pupal cuticle. Ultrastructural changes in the cuticle deposited before and after pupation correlate with the switch in cuticle protein synthesis. A similar biphasic accumulation of low and high molecular weight pupal cuticle proteins is also seen in imaginal discs cultured in vitro. The low molecular weight pupal cuticle proteins accumulate in response to a pulse of the insect steroid hormone 20-hydroxyecdysone and begin to appear 6 h after the withdrawal of the hormone from the culture medium. The high molecular weight pupal cuticle proteins accumulate later in culture; a second pulse of hormone appears to be necessary for the accumulation of two of these proteins.

Structure and expression of mRNA for a pupal cuticle protein of the silkworm, Bombyx mori

1990 ◽  
Vol 20 (7) ◽  
pp. 667-678 ◽  
Author(s):  
Hiroshi Nakato ◽  
Mayumi Toriyama ◽  
Susumu Izumi ◽  
Shiro Tomino
Keyword(s):  
Bombyx Mori ◽  
Cuticle Protein ◽  
Pupal Cuticle ◽  
Silkworm Bombyx Mori

Expression cloning and characterization of a pupal cuticle protein cDNA of Galleria mellonella L

1995 ◽  
Vol 25 (3) ◽  
pp. 355-363 ◽  
Author(s):  
Ursula Kollberg ◽  
Brigitte Obermaier ◽  
Harry Hirsch ◽  
Gisela Kelber ◽  
Peter Wolbert
Keyword(s):  
Galleria Mellonella ◽  
Expression Cloning ◽  
Cuticle Protein ◽  
Pupal Cuticle

scholarly journals Prepupal differentiation of Drosophila imaginal discs: identification of four genes whose transcripts accumulate in response to a pulse of 20-hydroxyecdysone.

Genetics ◽  
1988 ◽  
Vol 120 (2) ◽  
pp. 465-474
Author(s):  
K Fechtel ◽  
J E Natzle ◽  
E E Brown ◽  
J W Fristrom
Keyword(s):  
Steroid Hormone ◽  
Imaginal Discs ◽  
Prepupal Stage ◽  
Transcript Accumulation ◽  
Stage Of Development ◽  
Genes Encoding ◽  
Cuticle Protein ◽  
Continuous Presence ◽  
Pupal Cuticle

Abstract We have isolated and initially characterized a novel set of four genes expressed during the prepupal differentiation of imaginal discs of Drosophila melanogaster. These four ecdysone-dependent genes are named EDG-42A, EDG-64CD, EDG-78E and EDG-84A-1 based on their respective chromosomal locations. Their expression is like that expected for genes encoding proteins that participate in the formation of the pupal cuticle. Transcripts complementary to these genes accumulate in imaginal discs during an 18-hr in vitro culture period that begins with a 6-hr pulse of 20-hydroxyecdysone (20-HE). Transcripts for three of these genes were not detected in imaginal discs following culture in the absence or the continuous presence of 20-HE (1 microgram/ml). Transcripts corresponding to EDG-64CD exhibit delayed accumulation in the continuous presence of 20-HE. Transcripts corresponding to three of the genes are only detected in the prepupal stage of development. Only EDG-64CD is complementary to transcripts present at other stages of development. One of the genes, EDG-78E, encodes a pupal cuticle protein. This is the first reported isolation of a set of steroid hormone-responsive genes that require first the presence, then removal of hormone for transcript accumulation.

scholarly journals A Cluster of Cuticle Protein Genes of Drosophila melanogaster at 65A: Sequence, Structure and Evolution

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1213-1224
Author(s):  
Jean-Philippe Charles ◽  
Carol Chihara ◽  
Shamim Nejad ◽  
Lynn M Riddiford
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Dna ◽  
Multiple Copy ◽  
Sequence Structure ◽  
Coding Regions ◽  
The Third ◽  
Genetic Complexity ◽  
Genes Encoding ◽  
Cuticle Protein ◽  
Cuticle Proteins

A 36-kb genomic DNA segment of the Drosophila melanogaster genome containing 12 clustered cuticle genes has been mapped and partially sequenced. The cluster maps at 65A 5-6 on the left arm of the third chromosome, in agreement with the previously determined location of a putative cluster encompassing the genes for the third instar larval cuticle proteins LCP5, LCP6 and LCP8. This cluster is the largest cuticle gene cluster discovered to date and shows a number of surprising features that explain in part the genetic complexity of the LCP5, LCP6 and LCP8 loci. The genes encoding LCP5 and LCP8 are multiple copy genes and the presence of extensive similarity in their coding regions gives the first evidence for gene conversion in cuticle genes. In addition, five genes in the cluster are intronless. Four of these five have arisen by retroposition. The other genes in the cluster have a single intron located at an unusual location for insect cuticle genes.

Effects of catecholamines and β-alanine on tanning of pupal cuticle of Manduca sexta in vitro

1986 ◽  
Vol 32 (5) ◽  
pp. 473-478 ◽  
Author(s):  
Craig R Roseland ◽  
Janell M Green ◽  
Lynn M Riddiford
Keyword(s):  
Manduca Sexta ◽  
Pupal Cuticle
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