scholarly journals A simple method for quantification of biotinidase activity in dried blood spot and its application to screening of biotinidase deficiency.

1987 ◽  
Vol 152 (4) ◽  
pp. 339-346 ◽  
Author(s):  
AKIHIRO YAMAGUCHI ◽  
MASARU FUKUSHI ◽  
OSAMU ARAI ◽  
YOSHIKIYO MIZUSHIMA ◽  
YASUMASA SATO ◽  
...  
Keyword(s):  
Biotinidase Deficiency ◽  
Dried Blood Spot ◽  
Simple Method ◽  
Biotinidase Activity ◽  
Blood Spot

Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers

2011 ◽  
Vol 83 (9) ◽  
pp. 1522-1529 ◽  
Author(s):  
Livia Melo Villar ◽  
Jaqueline Correia de Oliveira ◽  
Helena Medina Cruz ◽  
Clara Fumiko Tachibana Yoshida ◽  
Elisabeth Lampe ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dried Blood Spot ◽  
Simple Method ◽  
B Virus ◽  
Hepatitis B Virus Markers ◽  
Blood Spot

scholarly journals A LAMP-SNP Assay Detecting C580Y Mutation in Pfkelch13 Gene from Clinically Dried Blood Spot Samples

2021 ◽  
Vol 59 (1) ◽  
pp. 15-22
Author(s):  
Thunchanok Khammanee ◽  
Nongyao Sawangjaroen ◽  
Hansuk Buncherd ◽  
Aung Win Tun ◽  
Supinya Thanapongpichat
Keyword(s):  
Field Study ◽  
Artemisinin Resistance ◽  
Plasmodium Species ◽  
Dried Blood Spot ◽  
Clinical Samples ◽  
Simple Method ◽  
Infected Blood ◽  
And Storage ◽  
C580y Mutation ◽  
Blood Spot

Artemisinin resistance (ART) has been confirmed in Greater Mekong Sub-region countries. Currently, C580Y mutation on Pfkelch13 gene is known as the molecular marker for the detection of ART. Rapid and accurate detection of ART in field study is essential to guide malaria containment and elimination interventions. A simple method for collection of malaria-infected blood is to spot the blood on filter paper and is fast and easy for transportation and storage in the field study. This study aims to evaluate LAMP-SNP assay for C580Y mutation detection by introducing an extra mismatched nucleotide at the 3’ end of the FIP primer. The LAMP-SNP assay was performed in a water bath held at a temperature of 56°C for 45 min. LAMP-SNP products were interpreted by both gel-electrophoresis and HNB-visualized changes in color. The method was then tested with 120 P. falciparum DNA from dried blood spot samples. In comparing the LAMP-SNP assay results with those from DNA sequencing of the clinical samples, the 2 results fully agreed to detect C580Y. The sensitivity and specificity of the LAMP-SNP assay showed 100%. There were no cross-reactions with other Plasmodium species and other Pfkelch13 mutations. The LAMP-SNP assay performed in this study was rapid, reliable, and useful in detecting artemisinin resistance in the field study.

Quantitative Analytical Method for the Determination of Biotinidase Activity in Dried Blood Spot Samples

2015 ◽  
Vol 87 (20) ◽  
pp. 10573-10578 ◽  
Author(s):  
Eszter Szabó ◽  
Ildikó Szatmári ◽  
László Szőnyi ◽  
Zoltán Takáts
Keyword(s):  
Analytical Method ◽  
Dried Blood Spot ◽  
Biotinidase Activity ◽  
Blood Spot

scholarly journals Validation and Clinical Evaluation of a Novel Method To Measure Miltefosine in Leishmaniasis Patients Using Dried Blood Spot Sample Collection

2016 ◽  
Vol 60 (4) ◽  
pp. 2081-2089 ◽  
Author(s):  
A. E. Kip ◽  
H. Rosing ◽  
M. J. X. Hillebrand ◽  
S. Blesson ◽  
B. Mengesha ◽  
...  
Keyword(s):  
Venous Blood ◽  
Dried Blood Spot ◽  
Patient Specific ◽  
Pharmacokinetic Studies ◽  
Sample Collection ◽  
Limit Of Quantification ◽  
Simple Method ◽  
Linear Effect ◽  
Combination Treatments ◽  
Blood Spot

ABSTRACTTo facilitate future pharmacokinetic studies of combination treatments against leishmaniasis in remote regions in which the disease is endemic, a simple cheap sampling method is required for miltefosine quantification. The aims of this study were to validate a liquid chromatography-tandem mass spectrometry method to quantify miltefosine in dried blood spot (DBS) samples and to validate its use with Ethiopian patients with visceral leishmaniasis (VL). Since hematocrit (Ht) levels are typically severely decreased in VL patients, returning to normal during treatment, the method was evaluated over a range of clinically relevant Ht values. Miltefosine was extracted from DBS samples using a simple method of pretreatment with methanol, resulting in >97% recovery. The method was validated over a calibration range of 10 to 2,000 ng/ml, and accuracy and precision were within ±11.2% and ≤7.0% (≤19.1% at the lower limit of quantification), respectively. The method was accurate and precise for blood spot volumes between 10 and 30 μl and for Ht levels of 20 to 35%, although a linear effect of Ht levels on miltefosine quantification was observed in the bioanalytical validation. DBS samples were stable for at least 162 days at 37°C. Clinical validation of the method using paired DBS and plasma samples from 16 VL patients showed a median observed DBS/plasma miltefosine concentration ratio of 0.99, with good correlation (Pearson'sr= 0.946). Correcting for patient-specific Ht levels did not further improve the concordance between the sampling methods. This successfully validated method to quantify miltefosine in DBS samples was demonstrated to be a valid and practical alternative to venous blood sampling that can be applied in future miltefosine pharmacokinetic studies with leishmaniasis patients, without Ht correction.

scholarly journals Simple method for α1-antitrypsin deficiency screening by use of dried blood spot specimens

2000 ◽  
Vol 15 (6) ◽  
pp. 1111 ◽  
Author(s):  
X. Costa ◽  
R. Jardi ◽  
F. Rodriguez ◽  
M. Miravitlles ◽  
M. Cotrina ◽  
...  
Keyword(s):  
Dried Blood Spot ◽  
Simple Method ◽  
Antitrypsin Deficiency ◽  
Deficiency Screening ◽  
Blood Spot

A simple method to diagnose adrenoleukodystrophy using a dried blood spot on filter paper

1986 ◽  
Vol 159 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Hisahide Nishio ◽  
Soichi Kodama ◽  
Sumiyoshi Yokoyama ◽  
Tamotsu Matsuo ◽  
Takaya Mio ◽  
...  
Keyword(s):  
Filter Paper ◽  
Dried Blood Spot ◽  
Simple Method ◽  
Blood Spot
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