Fatty Acid Composition of Lipids in the Leaves of the Crops from Tropical and Temperate Areas : I. Isolation of lipids by silicic acid column chromatography and preliminary results on the fatty acid composition

The physico-chemical properties of the extracted oil were studied by the conventional methods. It was observed that Siyal Kanta grown under the soil and climatic condition of Bangladesh contains about 35% of pale yellow coloured oil. The total lipids were fractionated into three major lipid groups, neutral lipids, glycolipid and phospholipids by silicic acid column chromatography. Among the lipids, the neutral lipids were varied from 92.1-92.3%, glycolipid 5.5-5.8% and phospholipid 1.5-1.7% of the total oil of the lipid applied. The oil was also fractionated into mono-, di- and triglyceride by silicic acid column chromatography. The triglycerides were varied from 90.1-90.3%, diglycerides from 2.3-2.8% and monoglycerides from 1.5-1.8%. The saturated and unsaturated fatty acids present in the oil were separated and found to be 14.2-14.5% and 84.2-84.8% respectively depending on the areas in which the plant grows. The fatty acid compositions of the oil were analyzed by Gas Liquid Chromatography (GLC). The major fatty acids found in the oil were oleic acid (23%), linoleic acid (58%), palmetic acid (7%) and ricinoleic acid (10%). Key words: Siyal kanta seed oil; Glyceride; Lipid; Fatty acid. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9607 BJSIR 2011; 46(4): 561-564

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THE LIPIDS OF MOUSE BROWN FAT

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y62-189 ◽

1962 ◽

Vol 40 (1) ◽

pp. 1705-1715 ◽

Cited By ~ 3

Author(s):

W. A. Spencer ◽

G. Dempster

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Palmitoleic Acid ◽

Silicic Acid Column ◽

White Mouse ◽

Brown Fat ◽

Average Chain Length ◽

Silicic Acid Column Chromatography ◽

White Fat

The lipids of the interscapular brown fat of the white mouse have been examined by means of silicic acid column chromatography and quantitative paper chromatography. The fatty acid composition of the glycerides and phosphatides was determined by gas chromatography. Comparative studies of mouse brown and white fat are presented.The fatty acid composition of the glycerides of the two tissues was similar at all ages examined. In both tissues, the average chain length of the glyceride fatty acids increased with age. The glycerides of brown fat at all ages contained less palmitoleic acid and more stearic acid than the corresponding white fat.The adult brown fat contained more polyglycerophosphatide and less sphingomyelin than white fat. The fatty acid composition of the lecithins of the two tissues was very similar. The similarity of brown fat and liver phosphatides is discussed.

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THE LIPIDS OF MOUSE BROWN FAT

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o62-189 ◽

1962 ◽

Vol 40 (12) ◽

pp. 1705-1715 ◽

Cited By ~ 96

Author(s):

W. A. Spencer ◽

G. Dempster

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Palmitoleic Acid ◽

Silicic Acid Column ◽

White Mouse ◽

Brown Fat ◽

Average Chain Length ◽

Silicic Acid Column Chromatography ◽

White Fat

The lipids of the interscapular brown fat of the white mouse have been examined by means of silicic acid column chromatography and quantitative paper chromatography. The fatty acid composition of the glycerides and phosphatides was determined by gas chromatography. Comparative studies of mouse brown and white fat are presented.The fatty acid composition of the glycerides of the two tissues was similar at all ages examined. In both tissues, the average chain length of the glyceride fatty acids increased with age. The glycerides of brown fat at all ages contained less palmitoleic acid and more stearic acid than the corresponding white fat.The adult brown fat contained more polyglycerophosphatide and less sphingomyelin than white fat. The fatty acid composition of the lecithins of the two tissues was very similar. The similarity of brown fat and liver phosphatides is discussed.

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Studies on the Lipid Classes and Fatty Acid Compositions of Petuli (Trewia nudiflora Linn.) Seed oil

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v47i1.10716 ◽

2012 ◽

Vol 47 (1) ◽

pp. 105-108

Author(s):

M A Khaleque ◽

MA Islam ◽

A Akhter ◽

MA Hye

Keyword(s):

Fatty Acid ◽

Neutral Lipid ◽

Silicic Acid ◽

Column Chromatography ◽

Seed Oil ◽

Silicic Acid Column ◽

Lipid Classes ◽

Total Weight ◽

Silicic Acid Column Chromatography ◽

Fatty Acid Compositions

Studies were carried out on the lipid classes and fatty acid compositions of petuli seed oil. It was observed that petuli seed grown under the soil and climatic condition of Bangladesh contains about 22% pale yellow coloured oil. The total lipids were fractionated into three major lipid classes, neutral lipid glyco lipid and phospho lipid by silicic acid column chromatography. The neutral lipid was accounted to 92.5% of the total weight of the lipid applied. The oil was also fractionated into mono, di and triglyceride by silicic acid column chromatography. The triglyceride was counted for over 90% of the total weight of the oil. The fatty acid compositions of the oil were analyzed by Gas liquid Chromatography and found the major fatty acid ?-elaeostearic acid 38.50%, oleic acid 34.35%, linoleic acid 26.15% and small amount of arachidic acid 1%. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10716 Bangladesh J. Sci. Ind. Res. 47(1), 105-108, 2012

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Microdetermination of Cholesterol and Phospholipid in Cerebrospinal Fluid and Serum by Silicic Acid Column Chromatography

Clinical Chemistry ◽

10.1093/clinchem/8.6.598 ◽

1962 ◽

Vol 8 (6) ◽

pp. 598-605 ◽

Cited By ~ 5

Author(s):

Yung S Shin ◽

James C Lee

Keyword(s):

Cerebrospinal Fluid ◽

Human Serum ◽

Total Phosphorus ◽

Silicic Acid ◽

Column Chromatography ◽

Silicic Acid Column ◽

Lipid Phosphorus ◽

Silicic Acid Column Chromatography ◽

Phospholipid Ratio

Abstract A method is presented for the determination of cholesterol and phospholipid, which requires 5 µl. of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 µg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 µg. of cholesterol and 30.5 µg. of phospholipid was 98.4 and 96.7%, respectively. Standard deviations of ± 1.73 and ± 1.24 have been obtained for the reproducibility of cholesterol and phospholipid determinations after chromatography. The method has been applied for the estimation of the cholesterol/phospholipid ratio and of lipid phosphorus in total phosphorus of human cerebrospinal fluids.

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