An 8-Year Longitudinal Survey for the Presence of Antibodies to Chicken Infectious Anemia Virus in Two Specific-Pathogen-Free Strains of Chickens

The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV.

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Coinfection of Specific-Pathogen-Free Chickens with Marek's Disease Virus (MDV) and Chicken Infectious Anemia Virus: Effect of MDV Pathotype

Avian Diseases ◽

10.2307/1593006 ◽

2001 ◽

Vol 45 (1) ◽

pp. 9 ◽

Cited By ~ 24

Author(s):

Andrea M. Miles ◽

Sanjay M. Reddy ◽

Robin W. Morgan

Keyword(s):

Disease Virus ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Specific Pathogen Free ◽

Chicken Infectious Anemia Virus ◽

Specific Pathogen ◽

Chicken Infectious Anemia ◽

Anemia Virus

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SEROLOGICAL STUDIES ON CHICKEN INFECTIOUS ANEMIA VIRUS IN GHARBEIA PROVINCE

Kafrelsheikh Veterinary Medical Journal ◽

10.21608/kvmj.2008.116233 ◽

2008 ◽

Vol 6 (2) ◽

pp. 196-208

Author(s):

Moshira, A. El-abasy ◽

Gamal, R. Hassab El-nabby ◽

Ahmed, S. Hegasy ◽

Salwa, M. Helmy

Keyword(s):

Chicken Infectious Anemia Virus ◽

Serological Studies ◽

Chicken Infectious Anemia ◽

Anemia Virus

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Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽

10.3390/ani11040944 ◽

2021 ◽

Vol 11 (4) ◽

pp. 944

Author(s):

Giulia Quaglia ◽

Giulia Mescolini ◽

Elena Catelli ◽

Giacomo Berto ◽

Filippo Muccioli ◽

...

Keyword(s):

Dna Amplification ◽

Molecular Characteristics ◽

Distribution Map ◽

Present Survey ◽

Broiler Breeder ◽

Non Invasive ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus ◽

Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

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Depletion of CD4 + and CD8 + T Lymphocyte Subpopulations by CIA-1, a Chicken Infectious Anemia Virus

Avian Diseases ◽

10.2307/1591677 ◽

1993 ◽

Vol 37 (2) ◽

pp. 492 ◽

Cited By ~ 26

Author(s):

Liang-biao Hu ◽

Benjamin Lucio ◽

Karel A. Schat

Keyword(s):

T Lymphocyte ◽

Lymphocyte Subpopulations ◽

T Lymphocyte Subpopulations ◽

Cd8 T Lymphocyte ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus

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Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1

Journal of General Virology ◽

10.1099/vir.0.2008/003103-0 ◽

2008 ◽

Vol 89 (12) ◽

pp. 2998-3003 ◽

Cited By ~ 12

Author(s):

Myrna M. Miller ◽

Keith W. Jarosinski ◽

Karel A. Schat

Keyword(s):

Fluorescent Protein ◽

Transcription Start ◽

Upstream Promoter ◽

Chicken Infectious Anemia Virus ◽

E Box ◽

Promoter Construct ◽

Chicken Infectious Anemia ◽

Lmh Cells ◽

Green Fluorescent ◽

Anemia Virus

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (δEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and δEF1.

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