scholarly journals Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1

2008 ◽  
Vol 89 (12) ◽  
pp. 2998-3003 ◽  
Author(s):  
Myrna M. Miller ◽  
Keith W. Jarosinski ◽  
Karel A. Schat
Keyword(s):  
Fluorescent Protein ◽  
Transcription Start ◽  
Upstream Promoter ◽  
Chicken Infectious Anemia Virus ◽  
E Box ◽  
Promoter Construct ◽  
Chicken Infectious Anemia ◽  
Lmh Cells ◽  
Green Fluorescent ◽  
Anemia Virus

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (δEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and δEF1.

SEROLOGICAL STUDIES ON CHICKEN INFECTIOUS ANEMIA VIRUS IN GHARBEIA PROVINCE

2008 ◽  
Vol 6 (2) ◽  
pp. 196-208
Author(s):  
Moshira, A. El-abasy ◽  
Gamal, R. Hassab El-nabby ◽  
Ahmed, S. Hegasy ◽  
Salwa, M. Helmy
Keyword(s):  
Chicken Infectious Anemia Virus ◽  
Serological Studies ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 944
Author(s):  
Giulia Quaglia ◽  
Giulia Mescolini ◽  
Elena Catelli ◽  
Giacomo Berto ◽  
Filippo Muccioli ◽  
...  
Keyword(s):  
Dna Amplification ◽  
Molecular Characteristics ◽  
Distribution Map ◽  
Present Survey ◽  
Broiler Breeder ◽  
Non Invasive ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus ◽  
Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

Chicken Infectious Anemia Virus Infection in Israeli Commercial Flocks: Virus Amplification, Clinical Signs, Performance, and Antibody Status

10.1637/7072 ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 108-118 ◽  
Author(s):  
I. Davidson ◽  
M. Kedem ◽  
H. Borochovitz ◽  
N. Kass ◽  
G. Ayali ◽  
...  
Keyword(s):  
Virus Infection ◽  
Clinical Signs ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

Immune Complex Vaccines for Chicken Infectious Anemia Virus

2011 ◽  
Vol 6 (1) ◽  
pp. e21-e22
Author(s):  
Karel A Schat ◽  
Nelson Rodrigo da Silva Martins ◽  
Priscilla H O'Connell ◽  
Michael S Piepenbrink
Keyword(s):  
Immune Complex ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Isolation and Immortalization of MIP-GFP Neurons From the Hypothalamus

Endocrinology ◽  
2014 ◽  
Vol 155 (6) ◽  
pp. 2314-2319 ◽  
Author(s):  
Zi Chen Wang ◽  
Michael B. Wheeler ◽  
Denise D. Belsham
Keyword(s):  
Green Fluorescent Protein ◽  
Energy Homeostasis ◽  
Fluorescent Protein ◽  
Transgenic Animals ◽  
Unintended Effects ◽  
Hypothalamic Neurons ◽  
Related Peptide ◽  
Promoter Construct ◽  
Insulin Responsiveness ◽  
Green Fluorescent

The mouse insulin I promoter (MIP) construct was developed to eliminate the promoter activity detected with the rat insulin II promoter in specific hypothalamic neurons that may have unintended effects on glucose and energy homeostasis in transgenic models. Thus, the specificity of this novel construct must be validated prior to the widespread availability of derived Cre models. Although limited validation efforts have indicated a lack of MIP activity within neuronal tissue, the global immunohistochemical methodology used may not be specific enough to rule out the possibility of specific populations of neurons with MIP activity. To investigate possible MIP activity within the hypothalamus, primary hypothalamic isolates from MIP-green fluorescent protein reporter mice were analyzed after fluorescent-activated cell sorting. Primary hypothalamic neurons isolated from the MIP-green fluorescent protein mice were immortalized. Characterization detected the presence of hypothalamic neuropeptide Y (NPY) and agouti-related peptide, involved in the control of energy homeostasis, as well as confirmed insulin responsiveness in the cell lines. Moreover, because insulin was demonstrated to differentially regulate NPY expression within these MIP neurons, the promoter construct may be active in multiple hypothalamic NPY/agouti-related peptide subpopulations with unique physiological functions. MIP transgenic animals may therefore face similar limitations seen previously with rat insulin II promoter-based models.

scholarly journals Synergetic pathogenicity of Newcastle disease vaccines LaSota strain and contaminated chicken infectious anemia virus

2019 ◽  
Vol 98 (5) ◽  
pp. 1985-1992 ◽  
Author(s):  
Qi Su ◽  
Tuanjie Wang ◽  
Fanfeng Meng ◽  
Zhizhong Cui ◽  
Shuang Chang ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus
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