Plasma Protein Electrophoresis in Birds: Comparison of a Semiautomated Agarose Gel System With an Automated Capillary System

2013 ◽  
Vol 27 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Yannick Roman ◽  
Marie-Claude Bomsel-Demontoy ◽  
Julie Levrier ◽  
Daniel Chaste-Duvernoy ◽  
Michel Saint Jalme
Keyword(s):  
Plasma Protein ◽  
Protein Electrophoresis ◽  
Agarose Gel ◽  
Capillary System ◽  
Gel System

scholarly journals APPLICATION OF 3-HYDROXYBUTYRATE MEASUREMENT AND PLASMA PROTEIN ELECTROPHORESIS IN THE DIAGNOSIS OF ASPERGILLOSIS IN AFRICAN PENGUINS (SPHENISCUS DEMERSUS)

2018 ◽  
Vol 49 (3) ◽  
pp. 696-703 ◽  
Author(s):  
Guillaume Desoubeaux ◽  
Marilyn Rodriguez ◽  
Ellen Bronson ◽  
Gayle Sirpenski ◽  
Carolyn Cray
Keyword(s):  
Plasma Protein ◽  
Protein Electrophoresis ◽  
Spheniscus Demersus

scholarly journals Screening and Diagnosis of Monoclonal Gammopathies: An International Survey of Laboratory Practice

2017 ◽  
Vol 142 (4) ◽  
pp. 507-515 ◽  
Author(s):  
Jonathan R. Genzen ◽  
David L. Murray ◽  
Gyorgy Abel ◽  
Qing H. Meng ◽  
Richard J. Baltaro ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Light Chain ◽  
Monoclonal Gammopathy ◽  
Protein Electrophoresis ◽  
Agarose Gel ◽  
Laboratory Practice ◽  
Serum Free ◽  
Serum Free Light Chain ◽  
Capillary Zone ◽  
Screening And Diagnosis

Context.— Serum tests used for the screening and diagnosis of monoclonal gammopathies include serum protein electrophoresis (SPE; agarose gel or capillary zone), immunofixation (IFE) and immunosubtraction capillary electrophoresis, serum free light chains, quantitative immunoglobulins, and heavy/light–chain combinations. Urine protein electrophoresis and urine IFE may also be used to identify Bence-Jones proteinuria. Objective.— To assess current laboratory practice for monoclonal gammopathy testing. Design.— In April 2016, a voluntary questionnaire was distributed to 923 laboratories participating in a protein electrophoresis proficiency testing survey. Results.— Seven hundred seventy-four laboratories from 38 countries and regions completed the questionnaire (83.9% response rate; 774 of 923). The majority of participants (68.6%; 520 of 758) used agarose gel electrophoresis as their SPE method, whereas 31.4% (238 of 758) used capillary zone electrophoresis. The most common test approaches used in screening were SPE with reflex to IFE/immunosubtraction capillary electrophoresis (39.3%; 299 of 760); SPE only (19.1%; 145 of 760); SPE and IFE or immunosubtraction capillary electrophoresis (13.9%; 106 of 760); and SPE with IFE, serum free light chain, and quantitative immunoglobulins (11.8%; 90 of 760). Only 39.8% (305 of 767) of laboratories offered panel testing for ordering convenience. Although SPE was used by most laboratories in diagnosing new cases of myeloma, when laboratories reported the primary test used to follow patients with monoclonal gammopathy, only 55.7% (403 of 724) chose SPE, with the next most common selections being IFE (18.9%; 137 of 724), serum free light chain (11.7%; 85 of 724), and immunosubtraction capillary electrophoresis (2.1%; 15 of 724). Conclusions.— Ordering and testing practices for the screening and diagnosis of monoclonal gammopathy vary widely across laboratories. Improving utilization management and report content, as well as recognition and development of laboratory-directed testing guidelines, may serve to enhance the clinical value of testing.

PLASMA PROTEIN ELECTROPHORESIS AND SELECT ACUTE PHASE PROTEINS IN HEALTHY BONNETHEAD SHARKS (SPHYRNA TIBURO) UNDER MANAGED CARE

2016 ◽  
Vol 47 (4) ◽  
pp. 984-992 ◽  
Author(s):  
Michael W. Hyatt ◽  
Cara L. Field ◽  
Tonya M. Clauss ◽  
Kristopher L. Arheart ◽  
Carolyn Cray
Keyword(s):  
Managed Care ◽  
Acute Phase ◽  
Plasma Protein ◽  
Acute Phase Proteins ◽  
Protein Electrophoresis ◽  
Sphyrna Tiburo

Plasma Protein Electrophoresis of the Atlantic Loggerhead Sea Turtle, Carretta carretta

2004 ◽  
Vol 14 (3) ◽  
pp. 13-18 ◽  
Author(s):  
John C. Gicking ◽  
Allen M. Foley ◽  
Kendal E. Harr ◽  
Rose E. Raskin ◽  
Elliott Jacobson
Keyword(s):  
Plasma Protein ◽  
Sea Turtle ◽  
Protein Electrophoresis ◽  
Loggerhead Sea Turtle

scholarly journals Comparison of Bromcresol Green and Agarose Protein Electrophoresis for Quantitation of Serum Albumin in Multiple Myeloma

2007 ◽  
Vol 53 (6) ◽  
pp. 1099-1103 ◽  
Author(s):  
Christine LH Snozek ◽  
Amy K Saenger ◽  
Philip R Greipp ◽  
Sandra C Bryant ◽  
Robert A Kyle ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Serum Albumin ◽  
Staging System ◽  
Protein Electrophoresis ◽  
Modular System ◽  
Agarose Gel ◽  
Myeloma Patient ◽  
Clinically Significant ◽  
M Spike ◽  
Bromcresol Green

Abstract Background: The International Staging System for multiple myeloma has increased the importance of accurate measurement of serum albumin. Two common albumin assays, bromcresol green (BCG) and agarose gel protein electrophoresis (PEL), frequently yield discordant results, creating confusion regarding which assay is superior for use in myeloma. Methods: We measured albumin by BCG on a Roche Modular system, by PEL with a Helena SPIFE SPE Vis agarose gel, and by immunonephelometry performed on a Dade Behring BNII nephelometer. BCG and PEL were used to measure albumin in 5777 patient samples, and all 3 methods were used in an additional 252 samples. The clinical impact was assessed on 698 myeloma patient samples. Results: For sera with zero/low monoclonal immunoglobulin protein (M)-spike (0 to <15 g/L), results for both BCG and PEL correlated well to nephelometry, although median PEL results were 8 g/L lower than corresponding BCG measurements. Correlation between PEL and nephelometry or BCG diminished with increasing M-spike, with PEL eventually overestimating albumin compared with both other assays. IgG and IgA M-spikes showed significantly different effects on albumin discordance. For 35% of myeloma patients, discrepancy between BCG and PEL had a potentially clinically significant effect on staging, but no difference in group survival was found. Conclusions: Both BCG and PEL correlate well to nephelometry in sera with zero/low M-spikes. In the presence of larger M-spikes, PEL correlates poorly to nephelometry or BCG, whereas BCG compares well with nephelometry regardless of M-spike. Thus, albumin measurement can be performed reliably in myeloma patient sera by use of inexpensive, automated BCG assays.

scholarly journals Agarose gel serum protein electrophoresis in cats with and without lymphoma and preliminary results of tandem mass fingerprinting analysis

2011 ◽  
Vol 40 (2) ◽  
pp. 159-173 ◽  
Author(s):  
Magda Gerou-Ferriani ◽  
Alix R. McBrearty ◽  
Richard J. Burchmore ◽  
Kamburapola G.I. Jayawardena ◽  
P. David Eckersall ◽  
...  
Keyword(s):  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Tandem Mass ◽  
Agarose Gel ◽  
Preliminary Results ◽  
Fingerprinting Analysis

Plasma protein electrophoresis as a prognostic indicator in Aspergillus species-infected Gentoo penguins (Pygoscelis papua papua )

2017 ◽  
Vol 46 (4) ◽  
pp. 605-614 ◽  
Author(s):  
Adam D. Naylor ◽  
Simon J. Girling ◽  
Donna Brown ◽  
Claire G. Crompton ◽  
Romain Pizzi
Keyword(s):  
Plasma Protein ◽  
Prognostic Indicator ◽  
Protein Electrophoresis ◽  
Aspergillus Species ◽  
Pygoscelis Papua

scholarly journals Serum amyloid A and plasma protein electrophoresis fractions in farmed white-tailed deer

2019 ◽  
Vol 31 (3) ◽  
pp. 458-462
Author(s):  
Carolyn Cray ◽  
Roxanne I. Knibb ◽  
Jeffrey R. Knibb
Keyword(s):  
Plasma Protein ◽  
Serum Amyloid A ◽  
Assessment Tool ◽  
Clinical Pathology ◽  
Reference Interval ◽  
Protein Electrophoresis ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Automated Method ◽  
Robust Statistical Method

Tools to measure the acute-phase response have been utilized widely in veterinary medicine. Evaluation by plasma protein electrophoresis (PPEP) has become an increasingly common assay in veterinary clinical pathology. Commercial reagents for serum amyloid A (SAA) have been validated for use in a variety of wildlife species. We analyzed samples from 29 healthy fawns and 60 healthy adult farmed white-tailed deer (WTD; Odocoileus virginianus) using an automated assay for SAA and a semi-automated method for PPEP. The robust statistical method for reference interval generation was used. SAA levels in fawns (0.1–26 mg/L) were found to be significantly higher than those in adults (0.1–5 mg/L, p < 0.01). The mean total protein was significantly lower in fawns (48 ± 10 g/L, p < 0.01) than in adults (73 ±5 g/L). The albumin-to-globulin ratio was also lower in fawns (0.56 ± 0.14) than in adults (1.25 ± 0.19, p < 0.01). Changes in SAA levels were observed in a variety of clinically abnormal animals. The combined use of the automated and semi-automated assays in our study may provide an additional valuable assessment tool in the care of captive WTD populations, for research studies, and for monitoring free-ranging animals.

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