scholarly journals Evaluation of Endogenous Reference Genes ofBactrocera (Tetradacus) minaxby Gene Expression Profiling under Various Experimental Conditions

2014 ◽  
Vol 97 (2) ◽  
pp. 597-604 ◽  
Author(s):  
Zhi-Chuang Lü ◽  
Liu-Hao Wang ◽  
Rui-Lin Dai ◽  
Gui-Fen Zhang ◽  
Jian-Ying Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Reference Genes ◽  
Experimental Conditions ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

Gene Expression Analysis in Superficial Bladder Cancer: Comparison of Two Suitable Endogenous Reference Genes

2007 ◽  
Vol 1 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Paula A. Videira ◽  
Dário Ligeiro ◽  
Manuela Correia ◽  
Hélder Trindade
Keyword(s):  
Gene Expression ◽  
Bladder Cancer ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Superficial Bladder Cancer ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

Selection of endogenous reference genes for gene expression analysis in Leishmania major developmental stages

2007 ◽  
Vol 101 (2) ◽  
pp. 473-477 ◽  
Author(s):  
Meriam Ouakad ◽  
Narges Bahi-Jaber ◽  
Mehdi Chenik ◽  
Koussay Dellagi ◽  
Hechmi Louzir
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Leishmania Major ◽  
Developmental Stages ◽  
Endogenous Reference ◽  
Selection Of ◽  
Endogenous Reference Genes

Approach for defining endogenous reference genes in gene expression experiments

2004 ◽  
Vol 329 (2) ◽  
pp. 293-299 ◽  
Author(s):  
J.J Garcı́a-Vallejo ◽  
B Van het Hof ◽  
J Robben ◽  
J.A.E Van Wijk ◽  
I Van Die ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

scholarly journals Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf

2019 ◽  
Author(s):  
xiangyu long ◽  
Jilai Lu ◽  
Nat N. V. Kav ◽  
Yunxia Qin ◽  
Yongjun Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Reference Genes ◽  
Rubber Tree ◽  
Ubiquitin Proteasome System ◽  
New Genes ◽  
Ubiquitin Proteasome ◽  
Suitable Reference ◽  
Tree Leaf

Abstract Backgroud Gene expression profiling is increasingly applied to investigate molecular mechanisms for which, normalization with suitable reference genes is critical. Previously we have reported several suitable reference genes for laticifer samples from rubber, however, little is known about reference genes in leaf. Results The main objective of this current study was to identify some reference genes with stable expression patterns in leaf at various developmental stages, as well as during abiotic (temperature extremes) and biotic stresses. Gene expression profiling experiments in rubber tree leaf identified the ubiquitin-proteasome system as having excellent potential as reference genes. Among a total of 30 tested genes investigated, 24 new (including 11 genes involved in the ubiquitin-proteasome system), 4 previously identified and 2 specific genes, were further evaluated using quantitative real-time PCR. Our results indicated that the new genes had better stability of expression when compared with others. For instance, an ubiquitin conjugating enzyme (RG0099) and three ubiquitin-protein ligases (RG0928, RG2190 and RG0118) expressed stably in all samples, and were confirmed to be suitable reference genes in rubber tree leaf in four different conditions. Finally, we suggest that using more than one reference gene may be appropriate in gene expression studies when employing different software to normalize gene expression data. Conclusion Our findings have significant implications for the reliability of data obtained from genomics studies in rubber tree and perhaps in other species.

scholarly journals Validation of endogenous reference genes in rat cerebral cortex for RT-qPCR analyses in developmental toxicity studies

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7181 ◽  
Author(s):  
Louise Ramhøj ◽  
Marta Axelstad ◽  
Terje Svingen
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Reference Genes ◽  
In Utero ◽  
Rat Cerebral Cortex ◽  
In Utero Exposure ◽  
Relative Gene Expression ◽  
Endogenous Reference ◽  
Relative Gene ◽  
Endogenous Reference Genes

Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must be stably expressed across all the tested samples. Here, we have tested 10 common RGs for their expression stability in cerebral cortex from young rats after in utero exposure to thyroid hormone (TH) disrupting compounds. We found that all 10 RGs were stable according to the three algorithms geNorm, NormFinder and BestKeeper. The downstream target gene Pvalb was significantly downregulated in brains from young rats after in utero exposure to propylthiouracil (PTU), a medicinal drug inhibiting TH synthesis. Similar results were obtained regardless of which of the 10 RGs was used for normalization. Another potential gene affected by developmental TH disruption, Dio2, was either not affected, or significantly upregulated about 1.4-fold, depending on which RG was used for normalization. This highlights the importance of carefully selecting correct RGs for normalization and to take into account the sensitivity of the RT-qPCR method when reporting on changes to gene expression that are less than 1.5-fold. For future studies examining relative gene expression in rat cerebral cortex under toxicological conditions, we recommend using a combination of either Rps18/Rpl13a or Rps18/Ubc for normalization, but also continuously monitor any potential regulation of the RGs themselves following alterations to study protocols.

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