THE INFLUENCE OF GROWTH HORMONE ON THE NUCLEIC ACID CONTENT OF RAT LIVER AND MUSCLE

1956 ◽  
Vol 13 (4) ◽  
pp. 429-432 ◽  
Author(s):  
BARBARA J. GRAY
Keyword(s):  
Skeletal Muscle ◽  
Growth Hormone ◽  
Nucleic Acid ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Female Rats ◽  
Nucleic Acid Content ◽  
Pituitary Growth Hormone

SUMMARY Adult female rats were treated with purified pituitary growth hormone for 9 days, and the nucleic acid content of the liver and skeletal muscle determined. An increase was observed in the concentration of muscle ribonucleic acid, expressed per gram of nitrogen, as a result of the treatment. There were no changes in the ribonucleic acid concentration of liver. No changes were observed in the concentration of deoxyribonucleic acid in muscle or liver.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

scholarly journals Cytological and Biochemical Studies of Anucleolate Xenopus Larvae

1962 ◽  
Vol s3-103 (61) ◽  
pp. 25-35
Author(s):  
H. WALLACE
Keyword(s):  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Content ◽  
Tail Bud ◽  
Lethal Mutant ◽  
Cytoplasmic Rna ◽  
Biochemical Studies ◽  
And Function

A larval lethal mutant of Xenopus laevis lacks true nucleoli but possesses analogous intranuclear organelles, here termed blobs, which are smaller and more numerous than nucleoli. Cytochemical tests reveal that blobs (like nucleoli) contain ribonucleic acid (RNA), arginine, and alkaline phosphatase, but probably no Feulgen-positive material. Anucleolate larvae are deficient in cytoplasmic RNA. By biochemical methods the nucleic acid content of anucleolate embryos is found to be normal at the tail-bud stage, but does not increase after this. By the time they hatch, anucleolate larvae are deficient in both RNA and deoxyribonucleic acid (DNA). The implications of this and related mutations on the formation and function of the nucleolus are considered. The term ‘blob’ is justified in that it would be misleading to regard such organelles as nucleoli produced by normally latent organizers.

scholarly journals EFFECTS OF CORTISONE ON REGENERATING RAT LIVER

1954 ◽  
Vol 37 (4) ◽  
pp. 559-574 ◽  
Author(s):  
Stanley L. Einhorn ◽  
Erich Hirschberg ◽  
Alfred Gellhorn
Keyword(s):  
Nucleic Acid ◽  
Rat Liver ◽  
Acid Content ◽  
Acid Synthesis ◽  
Significant Degree ◽  
Liver Cells ◽  
Nucleic Acid Content ◽  
Cell Multiplication ◽  
Continuous Administration ◽  
Regenerating Rat Liver

The effects of continuous administration of cortisone on the metabolism of regenerating rat liver have been studied. Whereas the restoration of the weight of the liver after partial hepatectomy was not markedly affected by cortisone, the multiplication of cells was reduced to a significant degree after the first 2 days of regeneration. Liver restoration in terms of nucleic acids was similarly inhibited by cortisone. The results are consistent with the interpretation that the inhibition of cell multiplication in this system is dependent on and keeps pace with the inhibition of nucleic acid synthesis by this drug. At almost any time after hepatectomy, the nucleic acid content of the liver cells was the same in treated and in untreated animals. In ancillary studies, it was shown that cortisone caused the cells of regenerating liver to be increased in size and weight through the increased infiltration of lipids. Changes in water, protein, and carbohydrate content of the liver cells did not contribute to this increase in the weight of the cells. Since all animals were treated with cortisone for 5 days before hepatectomy, data were also obtained on the effect of this agent on the resting liver. This course of treatment brought about a significant decrease in the number of cells per unit wet weight and in the water content of the livers. The nucleic acid content of the cells at hepatectomy, on the other hand, was unchanged.

Specificity of corticotrophin-induced increase in mitochondrial ribonucleic acid

1964 ◽  
Vol 206 (5) ◽  
pp. 1156-1160 ◽  
Author(s):  
Morton B. Sigel ◽  
J. Thomas Dowling
Keyword(s):  
Nucleic Acid ◽  
Ribonucleic Acid ◽  
Nuclear Dna ◽  
Weight Ratio ◽  
Intermediary Metabolism ◽  
Female Rats ◽  
Nucleic Acid Content ◽  
Mitochondrial Rna ◽  
Percentage Increase ◽  
Specific Increase

Ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) were measured in subcellular fractions of pooled adrenals removed from rats 4 hr after injection of agents known to affect or suspected of altering adrenal intermediary metabolism. Corticotrophin (2 µ) increased mitochondrial RNA 29% over that of saline-injected animals and 74% over uninjected controls. Nuclear DNA and nuclear and microsomal RNA were unchanged. Thyrotrophin (2 µ), growth hormone (1 µ), chorionic gonadotrophin (500 µ), triiodothyronine (25 µg), p-dinitrophenol (50 µg), crude parathyroid hormone (10 µ), and vasopressin (0.4 µ) failed to affect either nucleic acid. The mitochondrial-adrenal weight ratio increased only in corticotrophin-treated animals. The percentage increase of mitochondrial RNA exceeded that of mitochondrial weight. Conjugated estrogen was the only other agent that produced a response. One, five, and ten micrograms increased DNA in spayed female rats while 50 µg produced no effect. Estrogen presumably affected cell division. A part of the sequential response of adrenal intermediary metabolism to corticotrophin includes a specific increase in the nucleic acid content of that organelle associated anatomically with steroidogenesis.

THE EFFECT OF GROWTH HORMONE ON THE NUCLEIC ACID CONTENT OF THE DEVELOPING CHICK EMBRYO1

Endocrinology ◽  
1953 ◽  
Vol 53 (5) ◽  
pp. 520-526 ◽  
Author(s):  
TUNG-YUE WANG ◽  
KUANG-MEI HSIEH ◽  
HERMAN T. BLUMENTHAL
Keyword(s):  
Growth Hormone ◽  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content
Sign in / Sign up

Export Citation Format

Share Document