RADIOAUTOGRAPHIC STUDIES ON THE LOCALIZATION OF 125I-LABELLED HUMAN LUTEINIZING AND GROWTH HORMONE IN IMMATURE MALE RATS

1969 ◽  
Vol 43 (1) ◽  
pp. 105-111 ◽  
Author(s):  
D. M. DE KRETSER ◽  
K. J. CATT ◽  
H. G. BURGER ◽  
G. C. SMITH
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Luteinizing Hormone ◽  
Human Growth Hormone ◽  
Light Microscope ◽  
Human Growth ◽  
Proximal Convoluted Tubule ◽  
Male Rats ◽  
Immature Male ◽  
Parenchymal Cells

SUMMARY Twenty-day-old male rats were injected intraperitoneally with either human luteinizing hormone (HLH) or human growth hormone (HGH) labelled with 125I. The localization of these hormones 1–2 hr. after injection was examined under the light microscope after radioautography. Major sites of localization of labelled LH were the interstitial cells of the testis and the proximal convoluted tubule of the kidney. Some hormone was also present in adipose tissue, hepatic parenchymal cells, the mesothelial lining of the peritoneum and underlying macrophages. HGH was localized principally in the proximal convoluted tubule of the kidney with some hormone present in liver, adipose tissue, and the suprarenal cortex.

The effect of synthetic fragment 31–44 of human growth hormone on glucose uptake by isolated adipose tissue

1983 ◽  
Vol 110 (3) ◽  
pp. 866-872 ◽  
Author(s):  
N.A. Yudaev ◽  
Yu.A. Pankov ◽  
Yu.M. Keda ◽  
E.T. Sazina ◽  
T.A. Osipova ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Glucose Uptake ◽  
Human Growth Hormone ◽  
Human Growth

scholarly journals Вплив гормону росту (rhGH) та Biolaminin 521 LN на проліферативну активність стовбурових клітин кота

2018 ◽  
pp. 143-148
Author(s):  
А. Й. Мазуркевич ◽  
В. В. Ковпак ◽  
О. С. Ковпак
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Growth Hormone ◽  
Adipose Tissue ◽  
Cardiac Muscle ◽  
Cell Cultures ◽  
Human Growth Hormone ◽  
Proliferative Activity ◽  
Recombinant Human Growth Hormone ◽  
Human Growth

Досліджено вплив гормону росту (rhGH) у різних концентраціях та Biolaminin 521 LN на проліферативну активність та генетичну стабільність стовбурових клітин, отриманих з кісткового мозку, жирової тканини та міокарду кота. Встановлено, що гормон росту у низьких концентраціях (10 нг/мл) позитивно впливає на проліферативну активність стовбурових клітин, отриманих із жирової тканини та міокарду кота. Разом із тим, на культуру стовбурових клітин кісткового мозку ефект низьких концентрацій rhGH був протилежний. Культивування стовбурових клітин за додавання Biolaminin 521 LN призвело до достовірного збільшення індексу проліферації у всіх досліджуваних культурах. За даними цитогенетичного аналізу встановлено, що додавання гормону росту у культуральне середовище не призводить до достовірного збільшення кількості генетичних помилок.  Водночас, додавання Biolaminin 521 LN призводить до зменшення кількості клітин із зміненим каріотипом (у порівнянні з контролем) у всіх досліджуваних культурах. The usage of cell technology in clinical practice requires a large amount of cell material. It in turn provokes the development of methods that will allow to obtain a greater amount of cell material for a much shorter period of time. According to literary sources it is known that the growth hormone and the Biolaminin LN 521 can have a positive influence on the mitotic activity of stem cells. Considering the differences in the cell composition of the cell cultures obtained from different tissues, the effects of recombinant human growth hormone (rhGH) and Biolaminin 521 LN will be different. Therefore, our aim was to study the effects of recombinant human growth hormone (rhGH) in various concentrations and to study the Biolaminin LN 521 for the proliferative activity of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat. The effects of recombinant human growth hormone (rhGH) in various concentrations and the effects of Biolaminin LN 521 for proliferative activity and genetic stability of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat were studied. It was found that the growth hormone has a positive effect on the proliferative activity of stem cells in cell cultures of adipose tissue and cardiac muscle of cat at low concentrations (10 ng/ml), while the effect on the cell culture of bone marrow stem cells was the opposite. According to the cytogenetic analysis it was found that adding recombinant human growth hormone to the culture media does not lead to a significant increase in the number of genetic errors, while adding the Biolaminin 521 LN leads to a decrease in the number of cells with altered karyotype (in comparison with control) in all the studied cell cultures.

Clonal origin of pituitary adenomas

1990 ◽  
Vol 73 (5) ◽  
pp. 731-735 ◽  
Author(s):  
Lee B. Jacoby ◽  
E. Tessa Hedley-Whyte ◽  
Karen Pulaski ◽  
Bernd R. Seizinger ◽  
Robert L. Martuza
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Single Cell ◽  
Human Growth Hormone ◽  
X Chromosome ◽  
Pituitary Adenomas ◽  
Follicle Stimulating Hormone ◽  
Pituitary Tumors ◽  
Human Growth ◽  
Stimulating Hormone

✓ Benign pituitary adenomas are among the most common neurosurgical tumors and account for a diversity of clinical syndromes due to their hormone content and release. To determine whether these tumors arise from a single cell or multiple cells, the authors studied X chromosome inactivation in deoxyribonucleic acid (DNA) isolated from pituitary adenomas in women. Tumors of three different hormonal subtypes were examined. One tumor contained cells immunoreactive for prolactin and human growth hormone; one tumor contained foci immunoreactive for the β-subunits of luteinizing hormone and follicle-stimulating hormone; and the third tumor had no immunoreactive prolactin, human growth hormone, β-subunits of thyroid-stimulating hormone, luteinizing hormone, or follicle-stimulating hormone, or the α-subunit. Analysis of the DNA revealed that, in each of the three pituitary tumors, one X chromosome was active in all cells and one X chromosome was inactive, indicating that each of these tumors was monoclonal in origin. It is concluded that clinically evident pituitary tumors arise from a genetic mutation in a single cell.

scholarly journals The Influence of Human Growth Hormone (GH) and Thyroxine (T4) on the Differentiation of Adipose Tissue in the Fetus

1993 ◽  
Vol 1 (5) ◽  
pp. 345-356 ◽  
Author(s):  
Gary J. Hausman ◽  
J. Thom Wright ◽  
Amanda Latimer ◽  
Rosetta Watson ◽  
Roy J. Martin
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Human Growth Hormone ◽  
Human Growth
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