Clonal origin of pituitary adenomas

1990 ◽  
Vol 73 (5) ◽  
pp. 731-735 ◽  
Author(s):  
Lee B. Jacoby ◽  
E. Tessa Hedley-Whyte ◽  
Karen Pulaski ◽  
Bernd R. Seizinger ◽  
Robert L. Martuza
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Single Cell ◽  
Human Growth Hormone ◽  
X Chromosome ◽  
Pituitary Adenomas ◽  
Follicle Stimulating Hormone ◽  
Pituitary Tumors ◽  
Human Growth ◽  
Stimulating Hormone

✓ Benign pituitary adenomas are among the most common neurosurgical tumors and account for a diversity of clinical syndromes due to their hormone content and release. To determine whether these tumors arise from a single cell or multiple cells, the authors studied X chromosome inactivation in deoxyribonucleic acid (DNA) isolated from pituitary adenomas in women. Tumors of three different hormonal subtypes were examined. One tumor contained cells immunoreactive for prolactin and human growth hormone; one tumor contained foci immunoreactive for the β-subunits of luteinizing hormone and follicle-stimulating hormone; and the third tumor had no immunoreactive prolactin, human growth hormone, β-subunits of thyroid-stimulating hormone, luteinizing hormone, or follicle-stimulating hormone, or the α-subunit. Analysis of the DNA revealed that, in each of the three pituitary tumors, one X chromosome was active in all cells and one X chromosome was inactive, indicating that each of these tumors was monoclonal in origin. It is concluded that clinically evident pituitary tumors arise from a genetic mutation in a single cell.

THE RAT OVARIAN AUGMENTATION METHOD FOR FOLLICLE STIMULATING HORMONE

1972 ◽  
Vol 70 (4) ◽  
pp. 647-653 ◽  
Author(s):  
Peter Christiansen
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Urine Sample ◽  
Positive Response ◽  
Follicle Stimulating Hormone ◽  
Human Growth ◽  
Ovarian Response ◽  
Thyroid Stimulating Hormone ◽  
Inert Material ◽  
Stimulating Hormone

ABSTRACT The specificity of the rat ovarian augmentation method (Steelman & Pohley 1953) for follicle stimulation hormone (FSH) has been studied. The addition of luteinizing hormone (LH), thyroid stimulating hormone (TSH), prolactin, ACTH and human growth hormone (GH) did not influence the ovarian response either to ovine or to human FSH. Urine extracts from 2 infants gave no positive response with a dose of one 24 hour urine sample per rat, indicating that inert material in the urine extracts does not influence the ovarian response.

RADIOAUTOGRAPHIC STUDIES ON THE LOCALIZATION OF 125I-LABELLED HUMAN LUTEINIZING AND GROWTH HORMONE IN IMMATURE MALE RATS

1969 ◽  
Vol 43 (1) ◽  
pp. 105-111 ◽  
Author(s):  
D. M. DE KRETSER ◽  
K. J. CATT ◽  
H. G. BURGER ◽  
G. C. SMITH
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Luteinizing Hormone ◽  
Human Growth Hormone ◽  
Light Microscope ◽  
Human Growth ◽  
Proximal Convoluted Tubule ◽  
Male Rats ◽  
Immature Male ◽  
Parenchymal Cells

SUMMARY Twenty-day-old male rats were injected intraperitoneally with either human luteinizing hormone (HLH) or human growth hormone (HGH) labelled with 125I. The localization of these hormones 1–2 hr. after injection was examined under the light microscope after radioautography. Major sites of localization of labelled LH were the interstitial cells of the testis and the proximal convoluted tubule of the kidney. Some hormone was also present in adipose tissue, hepatic parenchymal cells, the mesothelial lining of the peritoneum and underlying macrophages. HGH was localized principally in the proximal convoluted tubule of the kidney with some hormone present in liver, adipose tissue, and the suprarenal cortex.

Prolactin gene expression in human growth hormone-secreting pituitary adenomas

1990 ◽  
Vol 72 (6) ◽  
pp. 879-882 ◽  
Author(s):  
Takashi Nagaya ◽  
Hisao Seo ◽  
Akio Kuwayama ◽  
Tsuyoshi Sakurai ◽  
Nobuhiro Tsukamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Pituitary Adenomas ◽  
Tumor Tissue ◽  
Pituitary Tumors ◽  
Human Growth ◽  
Content Type ◽  
Messenger Ribonucleic Acid ◽  
Prolactin Gene ◽  
Adenoma Tissue

✓ To elucidate the mechanism of hyperprolactinemia often observed in patients with growth hormone (GH)-secreting pituitary adenomas, the presence of immunoreactive prolactin (ir-PRL) and prolactin (PRL) messenger ribonucleic acid (mRNA) in the tumor tissue was examined by immunohistochemistry and cytoplasmic dot hybridization. Hyperprolactinemia was observed in three of 18 patients with GH-secreting adenoma. The tumor tissue was demonstrated to contain ir-PRL in nine patients and PRL mRNA in 13. The presence of ir-PRL in the tumor tissue was always associated with positive PRL mRNA, indicating production of PRL in GH-secreting tumors. Among the three patients with hyperprolactinemia, both ir-PRL and PRL mRNA was revealed in the tumor tissue of one, PRL mRNA but not ir-PRL was detected in the adenoma tissue of another, and neither PRL mRNA nor ir-PRL was found in the tumor tissue of the third. The association of hyperprolactinemia with the presence of both ir-PRL and PRL mRNA or PRL mRNA alone is indicative of PRL production and secretion. However, the absence of ir-PRL and PRL mRNA in the tumor tissue may indicate that hyperprolactinemia is caused by the suppression of PRL inhibitory factor due to hypothalamic dysfunction by the tumor mass. Thus, the study of PRL gene expression and immunohistochemistry in GH-secreting adenomas is valuable to understanding the pathophysiology of pituitary tumors.

Somatostatin Receptors in Human Growth Hormone and Prolactin- Secreting Pituitary Adenomas

1985 ◽  
Vol 61 (1) ◽  
pp. 98-103 ◽  
Author(s):  
E. MOYSE ◽  
M. LE DAFNIET ◽  
J. EPELBAUM ◽  
P. PAGESY ◽  
F. PEILLON ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Pituitary Adenomas ◽  
Somatostatin Receptors ◽  
Human Growth
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