EFFECTS OF PROSTAGLANDIN F2α ON SOME REPRODUCTIVE PROCESSES OF HAMSTERS AND RATS

1972 ◽  
Vol 53 (2) ◽  
pp. 201-213 ◽  
Author(s):  
A. P. LABHSETWAR
Keyword(s):  
Prostaglandin F2α ◽  
Concomitant Administration ◽  
Corpora Lutea ◽  
Fallopian Tubes ◽  
Racemic Form ◽  
Endocrine Profile ◽  
Prostaglandin F ◽  
Blue Reaction ◽  
Egg Transport ◽  
Reproductive Processes

SUMMARY In an attempt to characterize the endocrine profile of prostaglandin F2α (PGF2α) in relation to the female reproductive system, the compound (racemic form) was administered to hamsters and rats in various reproductive states. The prostaglandin terminated pregnancy when given once a day either subcutaneously (50 μg/hamster) or orally (1·5–2 mg/hamster) from Days 4 to 6 of pregnancy inclusive, or as a single subcutaneous injection (50 μg/animal) on Day 4. In the rat, higher (500 μg/injection) and more frequent (twice daily) s.c. injections were required to get even foetal resorption. Concomitant administration of progesterone (4 mg/animal) in either species protected pregnancy. Prostaglandin F2α terminated pregnancy without interfering with the Pontamine blue reaction, suggesting that its antifertility effects were not mediated by inhibition of implantation. In both hamsters and rats the prostaglandin markedly reduced the size of deciduomata which could be restored to normal by administration of progesterone. Prostaglandin F2α delayed passage of zygotes through the Fallopian tubes in a proportion of rats but failed to accelerate egg transport in rats and hamsters. Furthermore, it caused a marked histological degeneration of the corpora lutea and induced formation of a fresh set of corpora lutea in pseudopregnant, pregnant and pseudopregnant—hysterectomized hamsters. These deleterious effects of prostaglandin were accompanied, in hamsters, by the appearance of freshly ovulated tubal ova. Most of the endocrine effects of PGF2α observed in this study can be accounted for by its luteolytic property.

PROSTAGLANDIN E2: ANALYSIS OF EFFECTS ON PREGNANCY AND CORPUS LUTEUM IN HAMSTERS AND RATS

1972 ◽  
Vol 71 (3_Suppl) ◽  
pp. S3-S32 ◽  
Author(s):  
A. P. Labhsetwar
Keyword(s):  
Combined Treatment ◽  
Corpora Lutea ◽  
Pregnant Rats ◽  
Plasma Progesterone ◽  
Implantation Sites ◽  
Near Term ◽  
Exogenous Progesterone ◽  
Blue Reaction ◽  
Egg Transport ◽  
Reproductive Processes

ABSTRACT Effects of prostaglandin (PG) E2 on some reproductive processes of hamsters and rats were studied. The PG exerted antifertility effects in either species although it was 4–10 times less potent than PGF2α. Antifertility doses of PGE2 caused morphological degeneration of corpora lutea, induced fresh ovulations during the course of the treatment, significantly decreased peripheral progesterone levels and inhibited development of deciduomata in response to trauma. Both pregnancy and decidual growth could be maintained by simultaneous administration of exogenous progesterone. PGE2 also decreased plasma progesterone concentration in pseudopregnant-hysterectomized hamsters although the decrease was not significant when compared with intact pregnant or pseudopregnant hamsters. PGE2 produced no demonstrable effects on egg transport in hamsters but may have induced delayed implantation in rats by causing tubal retention of zygotes. The antifertility doses of PGE2 but not of PGF2α inhibited implantation of a proportion of blastocysts as studied by the Pontamine blue reaction and adversely affected the spacing mechanism of blastocysts in the uterus significantly reducing the distance between the adjacent implantation sites. When PGs E2 and F2α were given together to pregnant rats in doses which were virtually ineffective individually, the combined treatment caused loss of pregnancy. Administration of PGE2 to near term hamsters produced premature littering but oxytocin in doses tried proved inactive. Antifertility doses of PGE2 produced diarrhoea in rats but not in hamsters. The results demonstrate that PGE2 shares the luteolytic property of PGF2α but in addition possesses several other properties not known to be shared by PGF2α.

PLASMA PROGESTERONE LEVELS IN GUINEA-PIGS ACTIVELY IMMUNIZED AGAINST PROSTAGLANDIN F2α, HYSTERECTOMIZED OR TREATED WITH INTRA-UTERINE INDOMETHACIN

1975 ◽  
Vol 67 (1) ◽  
pp. 81-88 ◽  
Author(s):  
N. L. POYSER ◽  
E. W. HORTON
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Guinea Pigs ◽  
Bovine Serum ◽  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Bovine Serum Albumin Conjugate ◽  
Prostaglandin F

SUMMARY Five guinea-pigs actively immunized against a prostaglandin F2α(PGF2α)–bovine serum albumin conjugate showed elongated oestrous cycles. During these, corpora lutea were maintained in a functional secretory state as indicated by plasma progesterone levels. The results are compatible with the view that the PGF2α antibodies neutralized the PGF2α released from the uterus and thus prevented its normal luteolytic effect. Similar patterns of progesterone secretion were observed in two hysterectomized animals and in two animals with intra-uterine implants of indomethacin.

EFFECT OF INTRA-UTERINE BEADS ALONE, OR COMBINED WITH OESTROGEN TREATMENT, ON UTERO-OVARIAN VENOUS PLASMA PROSTAGLANDIN F2α AND PROGESTERONE LEVELS IN THE GUINEA-PIG

1976 ◽  
Vol 68 (3) ◽  
pp. 445-451 ◽  
Author(s):  
F. R. BLATCHLEY ◽  
B. T. DONOVAN ◽  
N. L. POYSER
Keyword(s):  
Guinea Pig ◽  
Prostaglandin F2α ◽  
Uterine Horn ◽  
Corpora Lutea ◽  
Oestrogen Treatment ◽  
Luteal Regression ◽  
Oestradiol Benzoate ◽  
Prostaglandin F ◽  
And Control ◽  
Venous Plasma

SUMMARY The presence of beads in the guinea-pig uterus caused premature regression of the corpora lutea, and a corresponding decrease in utero-ovarian venous plasma levels of progesterone, by days 9–10 of the oestrous cycle. If the beads were placed in one horn only, premature luteal regression occurred only in the adjacent ovary. Induced luteal regression was preceded by an increased release of prostaglandin F2α (PGF2α) from the uterine horns which contained the beads. Oestradiol benzoate treatment of guinea-pigs carrying beads in one uterine horn overcame the unilateral effect and resulted in premature luteal regression in both ovaries. This was also associated with the early release of PGF2α from both uterine horns, though a difference in the timing of the release was apparent between the bead-containing (experimental) and control horns.

CONCENTRATION OF PROSTAGLANDIN F2α AND STEROIDS IN THE HUMAN CORPUS LUTEUM

1977 ◽  
Vol 73 (1) ◽  
pp. 115-122 ◽  
Author(s):  
I. A. SWANSTON ◽  
K. P. McNATTY ◽  
D. T. BAIRD
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Progesterone Concentration ◽  
Late Luteal Phase ◽  
Prostaglandin F ◽  
Human Corpus Luteum

SUMMARY The concentration of prostaglandin F2α (PGF2α), progesterone, pregnenolone, oestradiol-17β, oestrone, androstenedione and testosterone was measured in corpora lutea obtained from 40 women at various stages of the menstrual cycle. The concentration of PGF2α was significantly higher in corpora lutea immediately after ovulation (26·7 ± 3·9 (s.e.m.) ng/g, P < 0·005) and in corpora albicantia (16·3 ± 3·3 ng/g, P < 0·005) than at any other time during the luteal phase. There was no correlation between the concentration of PGF2α and that of any steroid. The progesterone concentration was highest in corpora lutea just after ovulation (24·9 ± 6·7 μg/g) and in early luteal groups (25·7 ± 6·8 μg/g) but declined significantly (P < 0·05) to its lowest level in corpora albicantia (1·82 ± 0·66 μg/g). The concentration of oestradiol-17β in the corpus luteum and luteal weight were significantly greater during the mid-luteal phase than at any other stage (concentration 282 ± 43 ng/g, P < 0·05; weight 1·86 ± 0·18 g, P < 0·005). The results indicate that regression of the human corpus luteum is not caused by a rise in the ovarian concentration of PGF2α in the late luteal phase of the cycle.

scholarly journals Peri-ovulatory endocrine regulation of the prostanoid pathways in the bovine uterus at early dioestrus

2017 ◽  
Vol 29 (3) ◽  
pp. 544 ◽  
Author(s):  
Milena Lopes Oliveira ◽  
Fabio Luiz D'Alexandri ◽  
Guilherme Pugliesi ◽  
Veerle Van Hoeck ◽  
Fernando Silveira Mesquita ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Transcript Abundance ◽  
Beef Cows ◽  
Corpora Lutea ◽  
Endocrine Regulation ◽  
Uterine Tissue ◽  
Ovulatory Follicle ◽  
Endocrine Profile ◽  
Prostaglandin Endoperoxide Synthase ◽  
Ovulatory Follicles

We hypothesised that different endocrine profiles associated with pre-ovulatory follicle (POF) size would impact on uterine prostanoid pathways and thereby modulate the histotroph composition. Beef cows (n = 15 per group) were hormonally manipulated to have small (SF-SCL group) or large (LF-LCL group) pre-ovulatory follicles (POF) and corpora lutea (CL). Seven days after induction of ovulation, animals were slaughtered and uterine tissues and flushings were collected for quantification of prostanoids. The POF and CL size and the circulating progesterone concentrations at Day 7 were greater (P < 0.05) in the LF-LCL cows than in the SF-SCL group, as expected. The abundance of 5 out of 19 genes involved in prostanoid regulation was different between groups. Transcript abundance of prostaglandin F2α, E2 and I2 synthases was upregulated (P < 0.05) and phospholipase A2 was downregulated (P < 0.05) in endometrium of the LF-LCL group. No difference (P > 0.1) in prostanoid concentrations in the endometrium or in uterine flushings was detected between groups. However, prostaglandin F2α and E2 concentrations in the uterine flushings were positively correlated with the abundance of transcripts for prostaglandin endoperoxide synthase 2 (0.779 and 0.865, respectively; P < 0.002). We conclude that endometrial gene expression related to prostanoid synthesis is modulated by the peri-ovulatory endocrine profile associated with POF size, but at early dioestrus differences in transcript abundance were not reflected in changes in prostanoid concentrations in the uterine tissue and fluid.

Effects of forskolin, luteinizing hormone and prostaglandin F2α on isolated rat corpora lutea

1986 ◽  
Vol 112 (4) ◽  
pp. 571-578 ◽  
Author(s):  
Monica G. Sender Baum ◽  
Kurt E. B. Ahrén
Keyword(s):  
Marked Effect ◽  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Camp Accumulation ◽  
Camp Production ◽  
Immature Rats ◽  
Prostaglandin F ◽  
Serum Gonadotropin ◽  
Camp Formation ◽  
Isolated Rat

Abstract. The diterpene forskolin increased in a dosedependent way cyclic AMP (cAMP) accumulation in isolated corpora lutea from immature rats injected with an ovulatory dose of pregnant mare's serum gonadotropin (PMSG). The cAMP increase was significant already after 1 min of incubation with forskolin, and cAMP continued to rise to a maximum at about 30–60 min, with a clear decrease after 240 min of incubation. The forskolin effect was more pronounced in very young corpora lutea (1-day-old) than in older corpora lutea. There was a clear discrepancy between the marked effect on cAMP accumulation by forskolin and the steroidogenic response when compared with the corresponding effects of LH. Forskolin also increased progesterone production, but this effect was marginal compared with that of LH. Prostaglandin F2α (PGF2α) did not influence the forskolin stimulated cAMP increase. PGF2α has previously been shown to inhibit the stimulatory effect of LH on cAMP formation in this type of corpora lutea. The fact that PGF2α did not inhibit the forskolin stimulated cAMP production indicates that PGF2α does not act directly on the adenylate cyclase.

THE EFFECT OF PROSTAGLANDIN F2α AND PROSTAGLANDIN E2 UPON LUTEAL FUNCTION AND OVULATION IN THE GUINEA-PIG

1972 ◽  
Vol 53 (3) ◽  
pp. 493-501 ◽  
Author(s):  
F. R. BLATCHLEY ◽  
B. T. DONOVAN
Keyword(s):  
Guinea Pigs ◽  
Day Treatment ◽  
Prostaglandin F2α ◽  
Prostaglandin E ◽  
Corpora Lutea ◽  
Histamine Dihydrochloride ◽  
Luteal Regression ◽  
Luteal Function ◽  
Prostaglandin F ◽  
Effect Of Treatment

SUMMARY The effect of treatment with prostaglandin F2α (PGF2α on luteal function was examined in hysterectomized guinea-pigs. Regression of corpora lutea was found to occur when 0·25 mg (or more) was injected daily for 3 days and ovulation usually ensued within 5 days after treatment. The administration of 1 mg PGF2α daily for 7 days caused marked luteolysis but ovulation did not occur. Ovulation was blocked in three of four intact female guinea-pigs given 1 mg PGF2α/day for 7 days from day 15, but took place normally in five females injected with 0·25 mg/day. Treatment of hysterectomized guinea-pigs with 0·62 mg adrenaline hydrochloride/day, 1·97 mg atropine sulphate/day or 0·52 mg histamine dihydrochloride/day did not cause luteal regression, while the injection of 0·25 mg or 1 mg prostaglandin E2 daily for 3 days was likewise ineffective.

INTRACELLULAR DISTRIBUTION OF CATHEPSIN D IN RAT CORPORA LUTEA IN RELATION TO REPRODUCTIVE STATE AND THE ACTION OF PROSTAGLANDIN F2α AND PROLACTIN

1977 ◽  
Vol 75 (2) ◽  
pp. 317-NP ◽  
Author(s):  
MICHAL LAHAV ◽  
RINA MEIDAN ◽  
A. AMSTERDAM ◽  
H. GEBAUER ◽  
H. R. LINDNER
Keyword(s):  
Cathepsin D ◽  
Specific Activity ◽  
Prostaglandin F2α ◽  
Intracellular Distribution ◽  
Corpora Lutea ◽  
Reproductive State ◽  
Lysosomal Fraction ◽  
Prostaglandin F ◽  
Release Ratio ◽  
Soluble Material

To examine whether lysosomal enzymes play a part in the involution of corpora lutea, cathepsin D was assayed in both the lysosomal fraction ('bound' cathepsin D) and the postlysosomal supernatant fluid ('free' enzyme), by measuring the increment in absorbance at 280 nm caused by acid-soluble material released from haemoglobin. From these data the release ratio (free: bound specific activity) was calculated. In corpora lutea on days 5 and 15 of pregnancy, the values for lysosome-bound specific activity (units of E280/h/mg protein) were 4·76 ± 0·51 and 5·00 ± 0·29 (s.e.m.), and the release ratios were 0·12 ± 0·02 and 0·11 ± 0·01 respectively. Similar values were obtained in newly formed corpora lutea of dioestrous and pro-oestrous rats, but on the day of oestrus the level of cathepsin D bound to lysosomes decreased to 2·66 ± 0·36 and the release ratio increased to 0·36± 0·03. On day 5 of prolactin-induced pseudopregnancy, the activities of cathepsin D in both cellular fractions resembled those of pregnancy. An even higher level of lysosome-bound cathepsin D (7·11 ± 0·47) with a relatively low level of free activity (release ratio 0·18 ± 0·02) was observed in lactating rats (day 7 of lactation), in the persistent corpora lutea of pregnancy. Administration of prostaglandin F2α (PGF2α) on days 4, 5 and 6 of lactation significantly decreased the level of lysosome-bound cathepsin D measured on day 7 in the persistent corpora lutea of pregnancy (3·81 ± 0·24) and increased the release ratio (0·40 ± 0·05). The intracellular distribution of acid phosphatase did not show a consistent relationship with the reproductive state. It appears that a decrease in the amount of lysosome-bound cathepsin D is associated with incipient luteolysis, that prolactin inhibits release of cathepsin D from the lysosomes and that PGF2α counteracts this action of prolactin.

Deciphering the luteal transcriptome: potential mechanisms mediating stage-specific luteolytic response of the corpus luteum to prostaglandin F2α

2011 ◽  
Vol 43 (8) ◽  
pp. 447-456 ◽  
Author(s):  
Mohan Mondal ◽  
Beau Schilling ◽  
Joe Folger ◽  
Juan Pedro Steibel ◽  
Heli Buchnick ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Bovine Genome ◽  
Prostaglandin F2α ◽  
Specific Gene ◽  
Corpora Lutea ◽  
Specific Response ◽  
Gene Expression Response ◽  
Prostaglandin F ◽  
Time Point

The objective of this study was to identify prostaglandin F2α (PG)-induced changes in the transcriptome of bovine corpora lutea (CL) that are specific to mature, PG-responsive (day 11) CL vs. developing (day 4) CL, which do not undergo luteolysis in response to PG administration. CL were collected at 0, 4, and 24 h after PG injection on days 4 and 11 of the estrous cycle ( n = 5 per day and time point), and microarray analysis was performed with GeneChip Bovine Genome Arrays. Data normalization was performed with affy package and significance testing with maanova from Bioconductor. Significance (relative to 0 h time point) was declared at fold change >2.0 or <0.5 and false discovery rate of <5%. At 4 and 24 h after PG, 221 (day 4) and 661 (day 11) and 248 (day 4) and 1,421 (day 11) regulated genes, respectively, were identified. The accentuated gene expression response in day 11 CL was accompanied by specific enrichment of PG-regulated genes in distinctive gene ontology categories (immune related and other), particularly at 24 h after injection. Specificity in putative transcription factor binding sites was observed among PG-regulated genes on day 11 vs. day 4, including a potential association of ETS transcription factors with acute PG-induced gene expression specific to day 11 CL. Temporal and PG-induced regulation of abundance of mRNA for ETS transcription factor family members linked to the stage-specific response to PG was not observed. Increased abundance of protein and/or mRNA for six PG-regulated putative ETS-responsive genes was noted in day 11 but not day 4 CL. Results reveal insight into stage-specific gene expression in bovine CL in response to PG and potential transcriptional mediators of luteolysis.

Prostaglandin F2α- and phorbol 12-myristate-13-acetate-stimulated progesterone production by cultured human luteal cells in the mid-luteal phase: prostaglandin F2α increases cytosolic Ca2+ and inositol phosphates

1992 ◽  
Vol 133 (3) ◽  
pp. 451-458 ◽  
Author(s):  
T. Endo ◽  
H. Watanabe ◽  
H. Yamamoto ◽  
S. Tanaka ◽  
M. Hashimoto
Keyword(s):  
Luteal Phase ◽  
Inositol Phosphates ◽  
Prostaglandin F2α ◽  
Free Calcium ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Kinase C ◽  
Prostaglandin F ◽  
Human Corpus Luteum

ABSTRACT While prostaglandin F2α (PGF2α) has been thought to be a natural luteolysin in non-primates, a luteolytic effect in the human corpus luteum is less evident. We therefore investigated the action of PGF2α on monolayer cultures of human luteal cells obtained from mid-luteal phase corpora lutea. PGF2α increased basal and human chorionic gonadotrophin (hCG)-stimulated progesterone production by human cultured luteal cells. A potent tumour-promoting phorbol ester, phorbol 12-myristate-13-acetate (PMA), also stimulated progesterone production by cultured human luteal cells. Although human luteal cells were incubated for 24 h with PMA, hCG was still able to stimulate the production of progesterone by PMA-pretreated cells. However, PMA pretreatment blocked the ability of PGF2α to stimulate progesterone production. It is possible that the luteotrophic effect of PGF2α may be mediated, in part, by the activation of protein kinase C. Addition of PGF2α to suspensions of human luteal cells preincubated with myo-[2-3H]inositol promoted an increase in labelled inositol phosphates. PGF2α also rapidly increased intracellular free Ca2+ in human luteal cells loaded with the fluorescent Ca2+ probe, fura-2. We conclude that PGF2α and PMA stimulate progesterone production and that PGF2α increases the intracellular free calcium and inositol phosphates of human cultured luteal cells in the mid-luteal phase. Journal of Endocrinology (1992) 133, 451–458

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