CONCENTRATIONS OF CYCLIC AMP IN RABBIT OVARIAN TISSUE DURING THE PREOVULATORY PERIOD AND PSEUDOPREGNANCY AFTER INDUCTION OF OVULATION BY ADMINISTRATION OF HUMAN CHORIONIC GONADOTROPHIN

1975 ◽  
Vol 65 (1) ◽  
pp. 73-82 ◽  
Author(s):  
A. K. GOFF ◽  
PATRICIA W. MAJOR
Keyword(s):  
Cyclic Amp ◽  
Dose Response ◽  
Ovarian Tissue ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Induction Of Ovulation ◽  
Relationship Of

SUMMARY Concentrations of cyclic AMP were measured in rabbit ovaries at various times after injection of an ovulatory dose of human chorionic gonadotrophin (HCG). A biphasic increase in cyclic AMP concentration occurred during the preovulatory period, with peaks 30 min and 3–4 h after HCG injection. Concentrations of cyclic AMP had returned to those observed in ovaries of control oestrous animals before the onset of ovulation 10–12 h after administration of HCG, and remained low throughout the period of pseudopregnancy. Concentrations of cyclic AMP in the newly formed and developing corpora lutea were similar to the concentrations observed in the remainder of the tissue during this period. No significant increase in cyclic AMP concentration was observed 7–9 days after initiation of ovulation. Concentrations of ATP were also investigated during the preovulatory period. The dose– response relationship of HCG to cyclic AMP production in oestrous rabbit ovaries was investigated.

Biosynthesis and accumulation of cadaverine and putrescine in rat ovary after administration of human chorionic gonadotrophin

1980 ◽  
Vol 95 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Anne-Charlotte Andersson ◽  
Stig Henningsson
Keyword(s):  
Dose Response ◽  
Diamine Oxidase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
First Report ◽  
Mammalian Tissues ◽  
Rat Ovary ◽  
Relationship Of

Abstract. In the ovaries of pre-pubertal rats stimulated by human chorionic gonadotrophin (hCG) the temporal changes in cadaverine and putrescine formation were investigated. In addition, the dose-response relationship of hCG and its effect on the diamine formation and the effect of hCG on the content of diamines and polyamines in the ovaries and the urine were studied. The results show that the ovary stimulated by hCG, in addition to putrescine, forms cadaverine at a highly increased rate. The elevated diamine formation was parallelled by an increased content of cadaverine and putrescine in the ovary. Treatment with aminoguanidine elevated the content of cadaverine in the ovary, suggesting that diamine oxidase has a role as a regulator of the intra-ovary level of cadaverine. These results confirm that cadaverine can be synthesized in an inducible manner in mammalian tissues. This is, virtually, the first report of elevated formation of cadaverine in response to an exogenous gonadotrophin.

CYCLIC AMP AND CYCLIC GMP ACCUMULATION BY ISOLATED HAMSTER GRANULOSA CELLS STIMULATED BY LH AND FSH

1978 ◽  
Vol 89 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Anastasia Makris ◽  
Kenneth J. Ryan
Keyword(s):  
Cyclic Amp ◽  
Granulosa Cells ◽  
Dose Response ◽  
Cyclic Gmp ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Cyclic Amp Accumulation ◽  
Relationship Of ◽  
Higher Doses ◽  
Cyclic Amp Synthesis

ABSTRACT Cyclic AMP and cyclic GMP accumulation in hamster granulosa cells as a function of gonadotrophin dose (LH or FSH) and time (0–30 min) was determined. The pattern of acute cyclic AMP accumulation was similar in LH and FSH stimulated granulosa cells, except that the cells were more sensitive to FSH than LH. There was a positive dose response relationship of cyclic AMP accumulation in LH and FSH stimulated cells. LH appeared to partially inhibit FSH stimulated cyclic AMP synthesis. Cyclic GMP accumulation was distinctly different in LH and FSH stimulated cells. An inverse dose response relationship of cyclic GMP to dose LH was observed, with only the lowest dose of LH (0.005 IU/ml) stimulating cyclic GMP synthesis. FSH at 0.005 IU/ml did not stimulate cyclic GMP synthesis, but at higher doses generated cyclic GMP in a positive dose-related manner. The results suggest that specificity of hormone action in granulosa cells may be governed in part by differential on cyclic AMP and cyclic GMP in these cells.

Underestimation of Dose - Response Relationship with Particular Reference to the Relationship between the Dietary Intake of Mercury and its Concentration in Blood

1988 ◽  
Vol 7 (2) ◽  
pp. 129-132 ◽  
Author(s):  
J.C. Sherlock ◽  
M.J. Quinn
Keyword(s):  
Linear Regression ◽  
Dietary Intake ◽  
Dose Response ◽  
Mercury Concentration ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Blood Mercury ◽  
Relationship Of ◽  
The Relationship ◽  
Blood Mercury Concentration

Wide discrepancies have been observed between controlled and uncontrolled intake studies of the relationship of blood mercury concentration to intake of mercury. The probable reason for the apparent discrepancies is that the within-subject variation of mercury intake in the uncontrolled studies was almost certainly considerably larger than the within-subject variation in blood mercury concentration; in these circumstances, the apparent slope obtained from a linear regression of blood mercury on intake will invariably be much smaller than the true slope. Studies of the exposure or intake of any substance should therefore include a consideration of the likely within-subject variation in the exposure or intake relative to that in the effect.

A model analysis for dose–response relationship of warfarin in Japanese children: An introduction of the SIZE parameter

2016 ◽  
Vol 31 (3) ◽  
pp. 234-241 ◽  
Author(s):  
Saki Nakamura ◽  
Nao Watanabe ◽  
Naoki Yoshimura ◽  
Sayaka Ozawa ◽  
Keiichi Hirono ◽  
...  
Keyword(s):  
Dose Response ◽  
Model Analysis ◽  
Response Relationship ◽  
Size Parameter ◽  
Dose Response Relationship ◽  
Japanese Children ◽  
Relationship Of

The Dose-Response Relationship of Ondansetron in the Prophylaxis of Pediatric Postoperative Emesis

1994 ◽  
Vol 81 (SUPPLEMENT) ◽  
pp. A1351
Author(s):  
M. F. Watcha ◽  
P. J. Bras ◽  
J. Pennant ◽  
G. Cieslak ◽  
D. Burnette
Keyword(s):  
Dose Response ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Relationship Of

scholarly journals Investigations on the dose–response relationship of combined exposure to low doses of three anti-androgens in Wistar rats

2017 ◽  
Vol 91 (12) ◽  
pp. 3961-3989 ◽  
Author(s):  
Steffen Schneider ◽  
Karma C. Fussell ◽  
Stephanie Melching-Kollmuss ◽  
Roland Buesen ◽  
Sibylle Gröters ◽  
...  
Keyword(s):  
Dose Response ◽  
Wistar Rats ◽  
Low Doses ◽  
Response Relationship ◽  
Combined Exposure ◽  
Dose Response Relationship ◽  
Relationship Of

scholarly journals Comparative Effectiveness of Calabadion and Sugammadex to Reverse Non-depolarizing Neuromuscular-blocking Agents

2015 ◽  
Vol 123 (6) ◽  
pp. 1337-1349 ◽  
Author(s):  
Friederike Haerter ◽  
Jeroen Cedric Peter Simons ◽  
Urs Foerster ◽  
Ingrid Moreno Duarte ◽  
Daniel Diaz-Gil ◽  
...  
Keyword(s):  
Dose Response ◽  
Urine Analysis ◽  
Ex Vivo ◽  
Neuromuscular Blocking ◽  
Response Relationship ◽  
Binding Assays ◽  
Dose Response Relationship ◽  
Competition Binding ◽  
Relationship Of

Abstract Background The authors evaluated the comparative effectiveness of calabadion 2 to reverse non-depolarizing neuromuscular-blocking agents (NMBAs) by binding and inactivation. Methods The dose–response relationship of drugs to reverse vecuronium-, rocuronium-, and cisatracurium-induced neuromuscular block (NMB) was evaluated in vitro (competition binding assays and urine analysis), ex vivo (n = 34; phrenic nerve hemidiaphragm preparation), and in vivo (n = 108; quadriceps femoris muscle of the rat). Cumulative dose–response curves of calabadions, neostigmine, or sugammadex were created ex vivo at a steady-state deep NMB. In living rats, the authors studied the dose–response relationship of the test drugs to reverse deep block under physiologic conditions, and they measured the amount of calabadion 2 excreted in the urine. Results In vitro experiments showed that calabadion 2 binds rocuronium with 89 times the affinity of sugammadex (Ka = 3.4 × 109 M−1 and Ka = 3.8 × 107 M−1). The results of urine analysis (proton nuclear magnetic resonance), competition binding assays, and ex vivo study obtained in the absence of metabolic deactivation are in accordance with an 1:1 binding ratio of sugammadex and calabadion 2 toward rocuronium. In living rats, calabadion 2 dose-dependently and rapidly reversed all NMBAs tested. The molar potency of calabadion 2 to reverse vecuronium and rocuronium was higher compared with that of sugammadex. Calabadion 2 was eliminated renally and did not affect blood pressure or heart rate. Conclusions Calabadion 2 reverses NMB induced by benzylisoquinolines and steroidal NMBAs in rats more effectively, i.e., faster than sugammadex. Calabadion 2 is eliminated in the urine and well tolerated in rats.

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