DIURNAL VARIATION IN PLASMA TESTOSTERONE AND CORTISOL IN RHESUS MONKEYS LIVING IN SOCIAL GROUPS

1978 ◽  
Vol 76 (1) ◽  
pp. 67-74 ◽  
Author(s):  
R. M. ROSE ◽  
T. P. GORDON ◽  
I. S. BERNSTEIN
Keyword(s):  
Diurnal Variation ◽  
Individual Variation ◽  
Rhesus Monkeys ◽  
Plasma Cortisol ◽  
Social Groups ◽  
Time Of Day ◽  
Plasma Testosterone ◽  
Diurnal Changes ◽  
Considerable Individual Variation ◽  
Male Rhesus

SUMMARY Concentrations of cortisol and testosterone in the plasma of adult male rhesus monkeys living in social groups were determined during a 27-h period. Capture and venipuncture of experienced, conditioned animals did not lead to a fall in the concentration of testosterone in the plasma during the 90 min immediately after removal from the group. Both plasma cortisol and plasma testosterone concentrations showed a significant fall and rise in samples collected every 3 h during a 27-h period, even though there was considerable individual variation. During the autumn breeding season, animals showed higher concentrations of testosterone at both 10.00 and 22.00 h compared with those observed at the same times during the summer. Even though animals demonstrated significant diurnal changes, testosterone samples withdrawn at the same time of day (10.00 h) on consecutive days were significantly correlated with one another (r = 0·65, n = 27, P < 0·01), suggesting the usefulness of sampling once a day to study potential environmental influences on plasma testosterone concentrations.

DIURNAL CHANGES IN PLASMA TESTOSTERONE AND STUDIES ON PLASMA CORTICOSTEROIDS IN NON-ANAESTHETIZED MALE RHESUS MONKEYS (MACACA MULATTA)

1974 ◽  
Vol 63 (2) ◽  
pp. 325-335 ◽  
Author(s):  
R. P. MICHAEL ◽  
K. D. R. SETCHELL ◽  
T. M. PLANT
Keyword(s):  
Rhesus Monkeys ◽  
Plasma Testosterone ◽  
Diurnal Changes ◽  
Intact Male ◽  
Testosterone Levels ◽  
Adrenocortical Activity ◽  
Peripheral Plasma ◽  
Basal Plasma ◽  
Competitive Protein Binding ◽  
Male Rhesus

SUMMARY The assays of testosterone and corticosteroids in plasma from adult male rhesus monkeys using competitive protein-binding and radioimmunoassay techniques are described. The radioimmunoassay for testosterone was conducted without chromatography and, therefore, additionally estimated 17β-hydroxy-5α-androstan-3-one (dihydrotestosterone). Levels of testosterone in the peripheral plasma of 14 intact male rhesus monkeys showed marked fluctuations over a period of 24 h. Concentrations of testosterone at 22.00 h (1776 ± 814 ( ± s.d.) ng/100 ml) were approximately double those at 08.00 h (858 ± 407 ng/100 ml), 12.00 h (898 ± 316 ng/100 ml) and 16.00 h (784 ± 530 ng/100 ml). Castration resulted in low plasma testosterone levels (85 ± 29 ng/100 ml), and the increases at 22.00 h were no longer observed. In intact males, the 'basal' plasma corticosteroidlevel(08.00 h) was 22·4 ± 6·0 μg/100 ml. Administration of synthetic corticotrophin raised plasma corticosteroid levels without changing plasma testosterone concentration. Because plasma testosterone levels were not related to changes in adrenocortical activity, the noctural rises appear to be due to changes in testicular secretion.

scholarly journals Shotgun Lipidomics Discovered Diurnal Regulation of Lipid Metabolism Linked to Insulin Sensitivity in Nondiabetic Men

2019 ◽  
Vol 105 (5) ◽  
pp. 1501-1514 ◽  
Author(s):  
Katharina Kessler ◽  
Mathias J Gerl ◽  
Silke Hornemann ◽  
Markus Damm ◽  
Christian Klose ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Insulin Sensitivity ◽  
Diurnal Variation ◽  
Saturated Fatty Acids ◽  
Insulin Response ◽  
Time Of Day ◽  
Diurnal Changes ◽  
High Fat ◽  
Shotgun Lipidomics ◽  
Meal Timing

Abstract Context Meal timing affects metabolic homeostasis and body weight, but how composition and timing of meals affect plasma lipidomics in humans is not well studied. Objective We used high throughput shotgun plasma lipidomics to investigate effects of timing of carbohydrate and fat intake on lipid metabolism and its relation to glycemic control. Design 29 nondiabetic men consumed (1) a high-carb test meal (MTT-HC) at 09.00 and a high-fat meal (MTT-HF) at 15.40; or (2) MTT-HF at 09.00 and MTT-HC at 15.40. Blood was sampled before and 180 minutes after completion of each MTT. Subcutaneous adipose tissue (SAT) was collected after overnight fast and both MTTs. Prior to each investigation day, participants consumed a 4-week isocaloric diet of the same composition: (1) high-carb meals until 13.30 and high-fat meals between 16.30 and 22:00 or (2) the inverse order. Results 12 hour daily lipid patterns showed a complex regulation by both the time of day (67.8%) and meal composition (55.4%). A third of lipids showed a diurnal variation in postprandial responses to the same meal with mostly higher responses in the morning than in the afternoon. Triacylglycerols containing shorter and more saturated fatty acids were enriched in the morning. SAT transcripts involved in fatty acid synthesis and desaturation showed no diurnal variation. Diurnal changes of 7 lipid classes were negatively associated with insulin sensitivity, but not with glucose and insulin response or insulin secretion. Conclusions This study identified postprandial plasma lipid profiles as being strongly affected by meal timing and associated with insulin sensitivity.

scholarly journals Sources of individual variation in plasma testosterone levels

2007 ◽  
Vol 363 (1497) ◽  
pp. 1711-1723 ◽  
Author(s):  
Bart Kempenaers ◽  
Anne Peters ◽  
Katharina Foerster
Keyword(s):  
Individual Variation ◽  
Critical Role ◽  
Time Of Day ◽  
Integrative Approach ◽  
Plasma Testosterone ◽  
Extrinsic Factors ◽  
Honest Signalling ◽  
Large Variability ◽  
Trade Offs ◽  
Potential Sources

The steroid hormone testosterone (T) plays a central role in the regulation of breeding in males, because many physiological, morphological and behavioural traits related to reproduction are T dependent. Moreover, in many seasonally breeding vertebrates, male plasma T levels typically show a pronounced peak during the breeding season. While such population-level patterns are fairly well worked out, the sources and the implications of the large variability in individual T levels within the seasonal cycle remain surprisingly little understood. Understanding the potential sources of individual variation in T levels is important for behavioural and evolutionary ecologists, for at least two reasons. First, in ‘honest signalling’ theory, T is hypothesized to play a critical role as the assumed factor that enforces honesty of the expression of sexually selected quality indicators. Second, T is often considered a key mediator of central life-history trade-offs, such as investment in survival versus reproduction or in mating versus parental care. Here, we discuss the patterns of within- and between-individual variation in male plasma T levels in free-living populations of birds. We argue that it is unclear whether this variability mainly reflects differences in underlying individual quality (intrinsic factors such as genetic or maternal effects) or in the environment (extrinsic factors including time of day, individual territorial status and past experience). Research in avian behavioural endocrinology has mainly focused on the effects of extrinsic factors, while other sources of variance are often ignored. We suggest that studies that use an integrative approach and investigate the relative importance of all potential sources of variation are essential for the interpretation of data on individual plasma T levels.

Plasma Testosterone, Dominance Rank and Aggressive Behaviour in Male Rhesus Monkeys

Nature ◽  
1971 ◽  
Vol 231 (5302) ◽  
pp. 366-368 ◽  
Author(s):  
ROBERT M. ROSE ◽  
JOHN W. HOLADAY ◽  
IRWIN S. BERNSTEIN
Keyword(s):  
Aggressive Behaviour ◽  
Rhesus Monkeys ◽  
Dominance Rank ◽  
Plasma Testosterone ◽  
Male Rhesus

scholarly journals RF9 Rescues Cortisol-Induced Repression of Testosterone Levels in Adult Male Macaques

2021 ◽  
Vol 12 ◽  
Author(s):  
Rahim Ullah ◽  
Rabia Naz ◽  
Aalia Batool ◽  
Madiha Wazir ◽  
Tanzil Ur Rahman ◽  
...  
Keyword(s):  
Adult Male ◽  
Plasma Cortisol ◽  
Sodium Succinate ◽  
Plasma Testosterone ◽  
Hpg Axis ◽  
Testosterone Levels ◽  
Potent Agonist ◽  
Single Blood Sample ◽  
Male Rhesus ◽  
Hydrocortisone Treatment

Cortisol inhibits hypothalamic-pituitary-gonadal (HPG) axis whereas RF9, a potent agonist of kisspeptin receptor (GPR54) activates HPG-axis during fasting-induced stress and under normal physiological conditions. However, the effect of RF9 on the cortisol-induced repressed HPG-axis is not studied yet. This study investigated whether exogenous cortisol-induced repression of the HPG-axis can be rescued by RF9. Six intact adult male rhesus monkeys (Macaca mulatta) habituated to chair-restraint were administered hydrocortisone sodium succinate at a rate of 20 mg/kg of body weight (BW) per day for 12 days. Single blood sample was taken by venipuncture from each animal on alternate days for hormones analyses. On experimental day 12, hydrocortisone treated monkeys received a single intravenous bolus of RF9 (n = 3) and vehicle (n = 3). The animals were bled for a period of 4 h at 60 min intervals from an indwelling cannula in the saphenous vein. RF9 was administered intravenously at the dose of 0.1 mg/kg BW immediately after taking 0 min sample. Plasma cortisol and testosterone concentrations were measured by using specific enzyme immunoassays. Hydrocortisone treatment increased plasma cortisol levels (P ≤ 0.0001) and decreased plasma testosterone (P ≤ 0.0127) levels. Interestingly, compared to vehicle, RF9 treatment significantly increased plasma testosterone levels at 120 min (P ≤ 0.0037), 180 min (P ≤ 0.0016), and 240 min (P ≤ 0.0001) intervals in the hydrocortisone treated monkeys. From these results, we concluded that RF9 administration relieves the suppressed HPG-axis in term of plasma testosterone levels in the cortisol treated monkeys.

THE EFFECT OF SOLDACTONA® (CANRENOATE-POTASSIUM) ON PLASMA TESTOSTERONE AND ANDROSTENEDIONE AND URINARY 17-KETOSTEROIDS AND 17-HYDROXYCORTICOSTEROIDS

1972 ◽  
Vol 70 (1) ◽  
pp. 104-112 ◽  
Author(s):  
John-Fredrik Dymling ◽  
Karl Olof Nilsson ◽  
Bernt Hökfelt
Keyword(s):  
Urinary Excretion ◽  
Diurnal Variation ◽  
Plasma Cortisol ◽  
Plasma Concentrations ◽  
Plasma Testosterone ◽  
Aldosterone Antagonist ◽  
Control Samples

ABSTRACT The aim of the present study was to investigate the effect of the aldosterone antagonist Soldactona® on the plasma concentrations of testosterone and androstenedione and the urinary excretion of 17-ketosteroids and 17-hydroxycorticosteroids. In the control samples, a diurnal variation of plasma testosterone similar to that of plasma cortisol was observed. Soldactona® caused a rapid and -marked fall in plasma testosterone and a decrease in the urinary 17-ketosteroids. No appreciable alterations could be demonstrated in the plasma androstenedione or the urinary 17-hydroxycorticosteroids. It is concluded that Soldactona® causes a decreased secretion of testosterone from the testes.

AN ANNUAL RHYTHM IN THE PLASMA TESTOSTERONE OF ADULT MALE RHESUS MONKEYS MAINTAINED IN THE LABORATORY

1974 ◽  
Vol 62 (2) ◽  
pp. 403-404 ◽  
Author(s):  
T. M. PLANT ◽  
D. ZUMPE ◽  
M. SAULS ◽  
R. P. MICHAEL
Keyword(s):  
Adult Male ◽  
Rhesus Monkeys ◽  
Plasma Testosterone ◽  
Annual Rhythm ◽  
Male Rhesus
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