DOSE REGIMENS OF HUMAN GROWTH HORMONE: EFFECTS OF CONTINUOUS INFUSION AND OF A GELATIN VEHICLE ON GROWTH IN RATS AND RATE OF ABSORPTION IN RABBITS

1980 ◽  
Vol 87 (2) ◽  
pp. 303-312 ◽  
Author(s):  
P. MARY COTES ◽  
W. A. BARTLETT ◽  
ROSE E. GAINES DAS ◽  
P. FLECKNELL ◽  
R. TERMEER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Plasma Levels ◽  
Time Course ◽  
Human Growth ◽  
Daily Injection ◽  
Glycine Buffer ◽  
Hormone Effects ◽  
Rate Of Absorption ◽  
Hypophysectomized Rats

Different methods for administration of human growth hormone (hGH) have been examined with a view to efficient use of the limited amounts of hGH at present available for clinical use. We found that in hypophysectomized rats (1) hGH administered by continuous subcutaneous infusion induced a greater increase in body weight (referred to throughout as growth) than hGH administered by intermittent (daily) injection and (2) intermittent injections of hGH dissolved in 16% gelatin induced more growth than hGH dissolved in a glycine buffer. It was further found that (1) hGH dissolved in 16% gelatin compared with hGH dissolved in a glycine buffer induced lower maximal levels of immunoreactive plasma hGH and between 7 and 9 h after treatment higher plasma levels when injected subcutaneously in rabbits, (2) 125I-labelled hGH added as a tracer to hGH in gelatin was removed more slowly from subcutaneous injection sites in rabbits than 125I-labelled hGH given with hGH in glycine buffer and (3) changes in the ratio of hGH to gelatin had little effect on the time-course of plasma levels of hGH in the rabbit. Addition of the protease inhibitors aprotinin or 6-aminohexanoic acid, to injection of hGH in gelatin or glycine did not induce any consistent increase in plasma levels of hGH.

Reduced and S-carboxymethylated human growth hormone: a probe for diabetogenic action

1984 ◽  
Vol 247 (5) ◽  
pp. E639-E644
Author(s):  
C. M. Cameron ◽  
J. L. Kostyo ◽  
J. A. Rillema ◽  
S. E. Gennick
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Mouse Mammary Gland ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Hypophysectomized Rats

The biological activity profile of reduced and S-carboxymethylated human growth hormone (RCM-hGH) was determined to establish its suitability for study of the diabetogenic property of hGH. RCM-hGH was found to have greatly attenuated in vivo growth-promoting activity in the 9-day weight-gain test in hypophysectomized rats (approximately 1%) and to have a similar low order of in vitro activity in stimulating amino acid incorporation into the protein of the isolated rat diaphragm. RCM-hGH also only had approximately 1% of the in vitro insulin-like activity of the native hormone on isolated adipose tissue from hypophysectomized rats. In contrast, RCM-hGH retained substantial in vivo diabetogenic activity in the ob/ob mouse, appearing to have approximately 50% of the activity of the native hormone. RCM-hGH was also found to retain significant, although attenuated (25%), in vitro lactogenic activity when tested for the ability to stimulate amino acid incorporation into a casein-rich protein fraction in mouse mammary gland explants. Because RCM-hGH exhibits a high degree of diabetogenic activity, although lacking significant anabolic or insulin-like activities, it will be useful as a "monovalent" probe for the study of the molecular mechanism of the diabetogenic action of GH.

scholarly journals Human Growth Hormone Induces SOCS3 and CIS mRNA Increase in the Hypothalamic Neurons of Hypophysectomized Rats

2004 ◽  
Vol 51 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Yoko KASAGI ◽  
Reiko TOKITA ◽  
Tomoko NAKATA ◽  
Toshihiro IMAKI ◽  
Shiro MINAMI
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hypothalamic Neurons ◽  
Hypophysectomized Rats

PREPARATION OF HUMAN GROWTH HORMONE

1961 ◽  
Vol 23 (3) ◽  
pp. 285-NP ◽  
Author(s):  
A. L. C. WALLACE ◽  
K. A. FERGUSON
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
High Yield ◽  
Pituitary Hormone ◽  
Simple Method ◽  
Anterior Pituitary Hormone ◽  
Epiphysial Cartilage ◽  
Diethylaminoethyl Cellulose ◽  
Hypophysectomized Rats

SUMMARY A simple method for the preparation of human growth hormone using chromatography on diethylaminoethyl-cellulose is described. Material prepared in this way, when assayed by growth of the tibial epiphysial cartilage in hypophysectomized rats, is at least as active as material prepared by published methods and is obtained in high yield. The only other anterior pituitary hormone activity present in any concentration is prolactin.

IN VITRO AND IN VIVO EFFECT OF GROWTH HORMONE ON ALDOSTERONE SECRETION

1960 ◽  
Vol 38 (1) ◽  
pp. 1069-1075
Author(s):  
O. J. Lucis ◽  
E. H. Venning
Keyword(s):  
Growth Hormone ◽  
Adrenal Gland ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Hypophysectomized Rats

Porcine, monkey, and human growth hormone have no effect on the in vitro secretion of aldosterone by the rat adrenal gland. When monkey growth hormone is injected into hypophysectomized rats, the adrenals of these animals secrete, under in vitro conditions, increased amounts of aldosterone with no change in the secretion rate of corticosterone. The plasma of these rats contains a substance which appears to stimulate the secretion of aldosterone in the adrenals of normal rats.

PLASMA LEVELS OF GROWTH HORMONE IN PATIENTS WITH DIABETES MELLITUS, HYPERCHOLESTEROLAEMIA AND LIVER DISEASES

1959 ◽  
Vol XXXII (IV) ◽  
pp. 480-490 ◽  
Author(s):  
Olof Forsman ◽  
Carl A. Gemzell
Keyword(s):  
Diabetes Mellitus ◽  
Growth Hormone ◽  
Plasma Level ◽  
Human Growth Hormone ◽  
Plasma Levels ◽  
Liver Diseases ◽  
Human Growth ◽  
Diabetic Patients ◽  
Elevated Plasma ◽  
Patients With Diabetes

ABSTRACT From the plasma of 30 out of 51 patients with various metabolic disorders a »growth-hormone-like« factor was concentrated which when injected into hypophysectomized rats increased the width of the tibial epiphysis. A similar factor had previously been concentrated from the plasma of acromegalic patients. It was tentatively concluded that this factor was identical with human growth hormone (HGH). Diabetes mellitus. Of 40 patients with diabetes mellitus 24 showed elevated plasma levels of human growth hormone (HGH). The frequency of elevated plasma levels was higher in young diabetics than in older diabetic patients; in many cases a nondiabetic complication was associated with an elevated level. In the plasma of 4 patients with diabetes mellitus and liver diseases an elevated plasma level of HGH was found while in 3 patients with Kimmelstiel-Wilson's syndrome no increase of HGH was found. Liver diseases. In the plasma of 4 patients with various liver diseases an elevated plasma level of HGH was found while in the plasma of another 4 patients no increase of HGH was found. Hypercholesterolaemia. In the plasma of 2 patients with familiar hypercholesterolaemia elevated levels of HGH was found while no HGH was found in a patient with an idiopathic type of the disease.

Early Localization of125I-Labeled Human Growth Hormone in Adrenals and Other Organs of Immature Hypophysectomized Rats

Endocrinology ◽  
1971 ◽  
Vol 88 (6) ◽  
pp. 1309-1317 ◽  
Author(s):  
H. E. MAYBERRY ◽  
J. L. VAN DEN BRANDE ◽  
J. J. VAN WYK ◽  
WILLIAM J. WADDELL
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hypophysectomized Rats

scholarly journals Effects of Human Growth Hormone on Lipid Metabolism in Hypophysectomized Rats

1999 ◽  
Vol 20 (5) ◽  
pp. 287-293
Author(s):  
MICHITOSHI SEKINE ◽  
HITOSHI SEKI ◽  
YOUICHI HARA ◽  
MUTSUO TAIJI
Keyword(s):  
Growth Hormone ◽  
Lipid Metabolism ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hypophysectomized Rats
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