NEGATIVE FEEDBACK OF SECRETION OF FOLLICLE-STIMULATING HORMONE BY THE PITUITARY GLAND OF DEVELOPING MALE RATS

1980 ◽  
Vol 87 (3) ◽  
pp. 401-407 ◽  
Author(s):  
A. R. SHETH ◽  
GEETA R. VANAGE ◽  
A. Y. VAZE ◽  
A. N. THAKUR
Keyword(s):  
Binding Capacity ◽  
Rabbit Antiserum ◽  
Male Rats ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Human Seminal Plasma ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Age Dependent

Rabbit antiserum to human seminal plasma inhibin (hSPI) was administered subcutaneously to developing male rats of 5, 10, 14, 17 and 24 days of age and the size of the endogenous FSH rise in serum was measured. The FSH levels were threefold higher on day 9 and 1·5-fold higher on days 14 and 18 when compared with levels in control rats treated with normal rabbit serum. Furthermore, the in-vitro binding capacity of pituitary plasma membrane to 125I-labelled hSPI declined with increase in age of the rats. Thus, the results of the present study suggest that the sensitivity of the testicular inhibin–FSH feedback relationship is related to age-dependent changes in pituitary binding of inhibin.

In vitro Binding Capacity of Cholesterol and Bile Salts by Partially Depolymerized Chitosans

2009 ◽  
Vol 4 (3) ◽  
pp. 126-135 ◽  
Author(s):  
Mamadouba Bangoura ◽  
Xia Wenshui ◽  
Zhang Jiali
Keyword(s):  
Bile Salts ◽  
Binding Capacity ◽  
In Vitro Binding ◽  
Vitro Binding

scholarly journals In vitro binding of mutagenic heterocyclic aromatic amines by Bifidobacterium pseudocatenulatum G4

2010 ◽  
Vol 1 (2) ◽  
pp. 149-154 ◽  
Author(s):  
F. Faridnia ◽  
A. Hussin ◽  
N. Saari ◽  
S. Mustafa ◽  
L. Yee ◽  
...  
Keyword(s):  
High Performance ◽  
Binding Capacity ◽  
Bifidobacterium Longum ◽  
Heterocyclic Amines ◽  
Heterocyclic Aromatic Amines ◽  
Human Origin ◽  
Bacterial Strains ◽  
In Vitro Binding ◽  
Vitro Binding

Consumption of probiotics has been associated with decreased risk of colon cancer and reported to have antimutagenic/ anti-carcinogenic properties. One possible mechanism for this effect involves physical binding of the mutagenic compounds, such as heterocyclic amines (HCAs), to the bacteria. Therefore, the objective of this study was to examine the binding capacity of bifidobacterial strains of human origin on mutagenic heterocyclic amines which are suspected to play a role in human cancers. In vitro binding of the mutagens Trp-p-2, IQ, MeIQx, 7,8DiMeIQx and PhIP by three bacterial strains in two media of different pH was analysed using high performance liquid chromatography. Bifidobacterium pseudocatenulatum G4 showed the highest decrease in the total HCAs content, followed by Bifidobacterium longum, and Escherichia coli. pH affects binding capacity; the highest binding was obtained at pH 6.8. Gram-positive tested strains were found to be consistently more effective than the gram-negative strain. There were significant decreases in the amount of HCAs in the presence of different cell concentrations of B. pseudocatenulatum G4; the highest decrease was detected at the concentration of 1010 cfu/ml. The results showed that HCAs were able to bind with all bacterial strains tested in vitro, thus it may be possible to decrease their absorption by human intestine and increase their elimination via faeces.

ABSENCE OF AN IMMUNOLOGICALLY DISTINCT FOLLICLE-STIMULATING HORMONE RELEASING FACTOR IN RAT STALK MEDIAN EMINENCE EXTRACTS

1976 ◽  
Vol 68 (1) ◽  
pp. 89-94 ◽  
Author(s):  
M. SHAHMANESH ◽  
S. L. JEFFCOATE
Keyword(s):  
Median Eminence ◽  
Male Rats ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Pituitary Tissue ◽  
Rat Pituitary ◽  
Pre Treatment ◽  
Lh Rh ◽  
Dose Levels

SUMMARY Rat stalk median eminence (SME) extract was incubated with various quantities of an antiserum raised against synthetic luteinizing hormone releasing hormone (LH-RH) and the resulting material was used at two or more dose levels to stimulate release of LH and FSH from anterior pituitary tissue, obtained from male rats, incubated in vitro. The resulting dose–response lines were used to obtain the LH- and FSH-releasing potency of the material, expressed as a percentage of SME extract pre-incubated with normal rabbit serum. Treatment with various doses of antiserum reduced LH-releasing and FSH-releasing potencies to a similar extent. Stalk median eminence extract and synthetic LH-RH were incubated with antiserum and directly compared in the same experiment in vitro. The changes in LH and FSH release caused by pre-treatment with antibody were similar for SME extract and synthetic LH-RH. In a final experiment, SME extract was passed through a column of Sepharose 2B particles to which was coupled anti-LH-RH antibody. The resulting material, when mixed with synthetic LH-RH and used to stimulate rat pituitary tissue in vitro caused a similar increase in the LH- and FSH-releasing potencies of the synthetic decapeptide. It is concluded that rat SME extract contains a single releasing factor for LH and FSH immunologically indistinguishable from synthetic LH-RH.

In Vitro Binding Capacity of Various Fiber Sources for Magnesium, Zinc, and Copper

1995 ◽  
Vol 43 (6) ◽  
pp. 1580-1584 ◽  
Author(s):  
Ahmed Idouraine ◽  
Bibi Z. Hassani ◽  
Saffiatu S. Claye ◽  
Charles W. Weber
Keyword(s):  
Binding Capacity ◽  
In Vitro Binding ◽  
Vitro Binding

scholarly journals In vitro binding capacity of organic (wheat bran and rice bran) and inorganic (perlite) sources for Mn, Zn, Cu, and Fe

2015 ◽  
Vol 45 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Ali Ghodrat ◽  
Akbar Yaghobfar ◽  
Yahya Ebrahimnezhad ◽  
Habib Aghdam Shahryar ◽  
Abolfazl Ghorbani
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Binding Capacity ◽  
In Vitro Binding ◽  
Vitro Binding

scholarly journals In vivo and in vitro binding of (-)-hydroxyhexamide, a major metabolite of acetohexamide, to rabbit serum.

1989 ◽  
Vol 12 (8) ◽  
pp. 503-507
Author(s):  
Yorishige IMAMURA ◽  
Hiroko ABE ◽  
Yuichiro KOJIMA ◽  
Masaki OTAGIRI
Keyword(s):  
Major Metabolite ◽  
Rabbit Serum ◽  
In Vitro Binding ◽  
Vitro Binding

Effects of fermentation on the structural characteristics and in vitro binding capacity of soluble dietary fiber from tea residues

LWT ◽  
2020 ◽  
Vol 131 ◽  
pp. 109818 ◽  
Author(s):  
Jiajun Chen ◽  
Hairong Huang ◽  
Yi Chen ◽  
Jianhua Xie ◽  
Yiming Song ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Binding Capacity ◽  
Structural Characteristics ◽  
Soluble Dietary Fiber ◽  
In Vitro Binding ◽  
Vitro Binding

scholarly journals In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate

2011 ◽  
Vol 12 (2) ◽  
pp. 1066-1080 ◽  
Author(s):  
Jauricque Ursulla Kongo-Dia-Moukala ◽  
Hui Zhang ◽  
Pierre Claver Irakoze
Keyword(s):  
Bile Acids ◽  
Protein Hydrolysate ◽  
Binding Capacity ◽  
In Vitro Binding ◽  
Corn Protein ◽  
Vitro Binding
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