scholarly journals In vitro binding of mutagenic heterocyclic aromatic amines by Bifidobacterium pseudocatenulatum G4

2010 ◽  
Vol 1 (2) ◽  
pp. 149-154 ◽  
Author(s):  
F. Faridnia ◽  
A. Hussin ◽  
N. Saari ◽  
S. Mustafa ◽  
L. Yee ◽  
...  
Keyword(s):  
High Performance ◽  
Binding Capacity ◽  
Bifidobacterium Longum ◽  
Heterocyclic Amines ◽  
Heterocyclic Aromatic Amines ◽  
Human Origin ◽  
Bacterial Strains ◽  
In Vitro Binding ◽  
Vitro Binding

Consumption of probiotics has been associated with decreased risk of colon cancer and reported to have antimutagenic/ anti-carcinogenic properties. One possible mechanism for this effect involves physical binding of the mutagenic compounds, such as heterocyclic amines (HCAs), to the bacteria. Therefore, the objective of this study was to examine the binding capacity of bifidobacterial strains of human origin on mutagenic heterocyclic amines which are suspected to play a role in human cancers. In vitro binding of the mutagens Trp-p-2, IQ, MeIQx, 7,8DiMeIQx and PhIP by three bacterial strains in two media of different pH was analysed using high performance liquid chromatography. Bifidobacterium pseudocatenulatum G4 showed the highest decrease in the total HCAs content, followed by Bifidobacterium longum, and Escherichia coli. pH affects binding capacity; the highest binding was obtained at pH 6.8. Gram-positive tested strains were found to be consistently more effective than the gram-negative strain. There were significant decreases in the amount of HCAs in the presence of different cell concentrations of B. pseudocatenulatum G4; the highest decrease was detected at the concentration of 1010 cfu/ml. The results showed that HCAs were able to bind with all bacterial strains tested in vitro, thus it may be possible to decrease their absorption by human intestine and increase their elimination via faeces.

In Vitro Binding Capacity of Various Fiber Sources for Magnesium, Zinc, and Copper

1995 ◽  
Vol 43 (6) ◽  
pp. 1580-1584 ◽  
Author(s):  
Ahmed Idouraine ◽  
Bibi Z. Hassani ◽  
Saffiatu S. Claye ◽  
Charles W. Weber
Keyword(s):  
Binding Capacity ◽  
In Vitro Binding ◽  
Vitro Binding

scholarly journals In vitro binding capacity of organic (wheat bran and rice bran) and inorganic (perlite) sources for Mn, Zn, Cu, and Fe

2015 ◽  
Vol 45 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Ali Ghodrat ◽  
Akbar Yaghobfar ◽  
Yahya Ebrahimnezhad ◽  
Habib Aghdam Shahryar ◽  
Abolfazl Ghorbani
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Binding Capacity ◽  
In Vitro Binding ◽  
Vitro Binding

Effects of fermentation on the structural characteristics and in vitro binding capacity of soluble dietary fiber from tea residues

LWT ◽  
2020 ◽  
Vol 131 ◽  
pp. 109818 ◽  
Author(s):  
Jiajun Chen ◽  
Hairong Huang ◽  
Yi Chen ◽  
Jianhua Xie ◽  
Yiming Song ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Binding Capacity ◽  
Structural Characteristics ◽  
Soluble Dietary Fiber ◽  
In Vitro Binding ◽  
Vitro Binding

NEGATIVE FEEDBACK OF SECRETION OF FOLLICLE-STIMULATING HORMONE BY THE PITUITARY GLAND OF DEVELOPING MALE RATS

1980 ◽  
Vol 87 (3) ◽  
pp. 401-407 ◽  
Author(s):  
A. R. SHETH ◽  
GEETA R. VANAGE ◽  
A. Y. VAZE ◽  
A. N. THAKUR
Keyword(s):  
Binding Capacity ◽  
Rabbit Antiserum ◽  
Male Rats ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Human Seminal Plasma ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Age Dependent

Rabbit antiserum to human seminal plasma inhibin (hSPI) was administered subcutaneously to developing male rats of 5, 10, 14, 17 and 24 days of age and the size of the endogenous FSH rise in serum was measured. The FSH levels were threefold higher on day 9 and 1·5-fold higher on days 14 and 18 when compared with levels in control rats treated with normal rabbit serum. Furthermore, the in-vitro binding capacity of pituitary plasma membrane to 125I-labelled hSPI declined with increase in age of the rats. Thus, the results of the present study suggest that the sensitivity of the testicular inhibin–FSH feedback relationship is related to age-dependent changes in pituitary binding of inhibin.

scholarly journals In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate

2011 ◽  
Vol 12 (2) ◽  
pp. 1066-1080 ◽  
Author(s):  
Jauricque Ursulla Kongo-Dia-Moukala ◽  
Hui Zhang ◽  
Pierre Claver Irakoze
Keyword(s):  
Bile Acids ◽  
Protein Hydrolysate ◽  
Binding Capacity ◽  
In Vitro Binding ◽  
Corn Protein ◽  
Vitro Binding

scholarly journals In vitro Binding Capacity of Wheat and Barley for Mn, Zn, Cu and Fe

2015 ◽  
Vol 9 (6) ◽  
pp. 56-60 ◽  
Author(s):  
Ali Ghodrat ◽  
Akbar Yaghobfar ◽  
Yahya Ebrahimnezhad ◽  
Habib Aghdam Shahryar ◽  
Abolfazl Ghorbani
Keyword(s):  
Small Intestine ◽  
Binding Capacity ◽  
Total Carbohydrate ◽  
Total Dietary Fiber ◽  
Physiological Conditions ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Two Samples ◽  
Mineral Binding

This study was carried out to determination of in vitro binding capacity of wheat and barley for Mn, Zn, Cu and Fe. For this reason wheat and barley were chemical analyzed for moisture, protein, ash, ether extract, total carbohydrate, NDF, ADF, ADL, crude fiber, viscosity, cellulose, hemicelluloses, total dietary fiber, and endogenous minerals. Mineral binding capacity of two samples for Mn, Zn, Fe, and Cu under sequential simulated physiological conditions of the stomach, small intestine, and colon was investigated and compared. Barley showed the higher mineral binding capacity in small intestine. Among the minerals, Fe exhibited the highest percentage of binding under the simulated physiological conditions of the small intestine and the lowest percentage of removing occurred in the stomach for both cereals. Cereals had an affinity for Fe>Cu>Zn>Mn. 

In Vitro and in Vivo Comparison of Binding of 99m-Tc-Iabeled Anti-CEA MAb F33-104 with 99m-Tc-labeled Anti-CEA MAb BW431/26

1999 ◽  
Vol 38 (04) ◽  
pp. 115-119
Author(s):  
N. Oriuchi ◽  
S. Sugiyama ◽  
M. Kuroki ◽  
Y. Matsuoka ◽  
S. Tanada ◽  
...  
Keyword(s):  
Nude Mice ◽  
Binding Capacity ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Cell Binding ◽  
Vitro Binding ◽  
Labeling Method ◽  
Human Colon Adenocarcinoma

Summary Aim: The purpose of this study was to assess the potential for radioimmunodetection (RAID) of murine anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb) F33-104 labeled with technetium-99m (99m-Tc) by a reduction-mediated labeling method. Methods: The binding capacity of 99m-Tc-labeled anti-CEA MAb F33-104 with CEA by means of in vitro procedures such as immunoradiometric assay and cell binding assay and the biodistribution of 99m-Tc-labeled anti-CEA MAb F33-104 in normal nude mice and nude mice bearing human colon adenocarcinoma LS180 tumor were investigated and compared with 99m-Tc-labeled anti-CEA MAb BW431/26. Results: The in vitro binding rate of 99m-Tc-labeled anti-CEA MAb F33-104 with CEA in solution and attached to the cell membrane was significantly higher than 99m-Tclabeled anti-CEA MAb BW431/261 (31.4 ± 0.95% vs. 11.9 ± 0.55% at 100 ng/mL of soluble CEA, 83.5 ± 2.84% vs. 54.0 ± 2.54% at 107 of LS 180 cells). In vivo, accumulation of 99m-Tc-labeled anti-CEA MAb F33-104 was higher at 18 h postinjection than 99m-Tc-labeled anti-CEA MAb BW431/26 (20.1 ± 3.50% ID/g vs. 14.4 ± 3.30% ID/g). 99m-Tcactivity in the kidneys of nude mice bearing tumor was higher at 18 h postinjection than at 3 h (12.8 ± 2.10% ID/g vs. 8.01 ± 2.40% ID/g of 99m-Tc-labeled anti-CEA MAb F33-104, 10.7 ± 1.70% ID/g vs. 8.10 ± 1.75% ID/g of 99m-Tc-labeled anti-CEA MAb BW431/26). Conclusion: 99m-Tc-labeled anti-CEA MAb F33-104 is a potential novel agent for RAID of recurrent colorectal cancer.

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