Suppression of ovine plasma FSH by bovine follicular fluid: neutralization by plasma from ewes immunized against an inhibin-enriched preparation from bovine follicular fluid

1986 ◽  
Vol 111 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S. A. R. Al-Obaidi ◽  
B. M. Bindon ◽  
M. A. Hillard ◽  
T. O'Shea ◽  
L. R. Piper
Keyword(s):  
Plasma Concentration ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Affinity Chromatography ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
Luteal Phase ◽  
Plasma Concentrations

ABSTRACT Adult Merino ewes were immunized against an inhibin-enriched preparation (bFFI) obtained by affinity chromatography of bovine follicular fluid (bFF). Plasma was obtained in early luteal phase from these ewes and from control ewes immunized against bovine serum albumin. Ten months after ovariectomy the plasma concentration of FSH, but not LH, in control ewes was decreased by four s.c. injections of 8 ml bFFI (17 500 units inhibin/injection). There was no decrease in plasma concentrations of FSH or LH in immunized ewes with the same dose of bFFI. In a second study with long-term ovariectomized ewes, four injections of 20 ml plasma from the immunized ewes significantly reduced the decrease in FSH concentration caused by four injections of steroid-free bFF (2500 units inhibin/injection) in comparison with similar ewes injected with plasma from control ewes. These results show that the plasma of ewes immunized against bFFI contains substances, presumably antibodies, which neutralize the FSH-suppressive effects of bFF and bFFI in ovariectomized ewes. J. Endocr. (1986) 111, 1–5

PLASMA CONCENTRATIONS OF LUTEINIZING HORMONE, FOLLICLE-STIMULATING HORMONE AND PROGESTERONE DURING THE BREEDING SEASON IN EWES IMMUNIZED AGAINST ANDROSTENEDIONE OR TESTOSTERONE

1979 ◽  
Vol 81 (3) ◽  
pp. 249-259 ◽  
Author(s):  
N. D. MARTENSZ ◽  
R. J. SCARAMUZZI
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Breeding Season ◽  
Bovine Serum ◽  
Luteal Phase ◽  
Follicle Stimulating Hormone ◽  
Plasma Concentrations ◽  
Biologically Active ◽  
Active Fraction ◽  
Lh Secretion

Levels of plasma LH, FSH and progesterone during the breeding season were measured by radioimmunoassay in control ewes and ewes actively immunized against androstenedione-11α-hemisuccinyl–bovine serum albumin or testosterone-3(O-carboxymethyl)oxime–bovine serum albumin. Immunization against androstenedione resulted in normal oestrous cycles with raised plasma LH and progesterone levels and a reduction in the concentration of FSH during the luteal phase. It is tentatively suggested that androstenedione, or its metabolites, could modify the oestrogenic control of LH secretion and facilitate the release of FSH in the ewe. Immunization against testosterone prevented oestrus and resulted in markedly increased levels of LH without alteration of the FSH concentration. Since evidence of increased binding of oestradiol-17β was found in the ewes immunized against testosterone, these results cannot be attributed solely to a reduction in the biologically active fraction of testosterone.

Biodistribution and long-term fate of silver nanoparticles functionalized with bovine serum albumin in rats

Metallomics ◽  
2010 ◽  
Vol 2 (3) ◽  
pp. 204-210 ◽  
Author(s):  
Lourdes Garza-Ocañas ◽  
Domingo A. Ferrer ◽  
Justin Burt ◽  
Luis A. Diaz-Torres ◽  
Mónica Ramírez Cabrera ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase

1985 ◽  
Vol 5 (12) ◽  
pp. 1071-1077 ◽  
Author(s):  
Geoffrey A. Stevenson ◽  
J. Guy Lyons ◽  
David A. Cameron ◽  
Robert L. O'Grady
Keyword(s):  
Epithelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mammary Carcinoma ◽  
Bovine Serum ◽  
Defined Medium ◽  
Carcinoma Cells ◽  
Serum Free ◽  
Vertebrate Collagenase

Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase. This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.

scholarly journals In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

2012 ◽  
Vol 41 (1) ◽  
pp. 6-12
Author(s):  
MAMY Khandoker ◽  
MMT Reza ◽  
LY Asad ◽  
S Saha ◽  
AS Apu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
In Vitro Maturation ◽  
Fertilization Rate ◽  
Maturation Rate ◽  
Normal Fertilization

The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilized by the buffalo oocytes and the fertilization rate was 23.28% to 30.52% in BFA and BSA supplemented media, respectively. It can be concluded that buffalo oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.http://dx.doi.org/10.3329/bjas.v41i1.11969

scholarly journals Studies of drug-protein binding by affinity chromatography. II. Interactions of bovine serum albumin with various ligands.

1979 ◽  
Vol 27 (9) ◽  
pp. 2048-2055 ◽  
Author(s):  
NAOMI I. NAKANO ◽  
TAKAYUKI OSHIO ◽  
NORIKO SATO ◽  
YOSHIMITSU SHIMAMORI ◽  
SHIGENORI YAMAGUCHI
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protein Binding ◽  
Affinity Chromatography ◽  
Bovine Serum ◽  
Drug Protein Binding
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