Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion

combination, in the NIH 3T3 cell line using in vitro assay, and compared with single HVCO and single fucoidan. Methods: NIH 3T3 Cell viability and proliferation were assessed using the MTT method, migration activity was assessed using scratch wound healing assays and expression of COX-2 and VEGF protein were determined using immunocytochemistry (ICC). Results: The results from the proliferative activity assay show that the effective concentrations for all samples were 31.25 μg /ml. NIH 3T3 cells migration activity assay showed that the best combination of the HVCO and fucoidan was 50:50. From COX 2 and VEGF protein expression test results, the combination of HVCO and fucoidan has a higher percentage of expression than single HVCO or single fucoidan Conclusion: The results reveal that the combination of HVCO and fucoidan has better wound healing activity than single HVCO or single fucoidan

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A direct in vitro assay for evaluation of diphtheria vaccine efficacy. Measurement of the competition of toxin and toxoids for neutralizing antibodies

Immunology Letters ◽

10.1016/s0165-2478(97)88020-0 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 293-294

Author(s):

A Wahby

Keyword(s):

Vaccine Efficacy ◽

Neutralizing Antibodies ◽

In Vitro Assay ◽

Vitro Assay

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In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651281 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 384-396 ◽

Cited By ~ 1

Author(s):

G Zbinden ◽

S Tomlin

Keyword(s):

Platelet Adhesion ◽

Sodium Citrate ◽

Blood Platelets ◽

In Vitro Assay ◽

Red Cells ◽

Platelet Adhesiveness ◽

Vitro Assay ◽

In Vitro System ◽

Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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A comparative study on adhesion and recovery of potential probiotic strains ofLactobacillusspp. by in vitro assay and analysis of human colon biopsies

Microbial Ecology in Health and Disease ◽

10.3402/mehd.v21i2.7563 ◽

2009 ◽

Vol 21 (2) ◽

Author(s):

Nadja Larsen ◽

Kim F. Michaelsen ◽

Anders Pærregaard ◽

Finn K. Vogensen ◽

Mogens Jakobsen

Keyword(s):

Comparative Study ◽

Human Colon ◽

In Vitro Assay ◽

Vitro Assay ◽

Probiotic Strains

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Antioxidant and anti-inflammatory properties of herbal medicine compound (KodiPur®) using in vitro assay

Agriculture and Natural Resources ◽

10.34044/j.anres.2019.53.2.15 ◽

2019 ◽

Keyword(s):

Herbal Medicine ◽

In Vitro Assay ◽

Vitro Assay ◽

Anti Inflammatory

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Angiogenic Effect of Pravastatin Alone and with Sera from Healthy and Complicated Pregnancies Studied by in vitro Vasculogenesis/Angiogenesis Assay

Journal of Vascular Research ◽

10.1159/000512831 ◽

2021 ◽

pp. 1-9

Author(s):

Anita Virtanen ◽

Outi Huttala ◽

Kati Tihtonen ◽

Tarja Toimela ◽

Tuula Heinonen ◽

...

Keyword(s):

Direct Effect ◽

Late Onset ◽

Maternal Serum ◽

In Vitro Assay ◽

Serum Samples ◽

Therapeutic Concentration ◽

Tubule Formation ◽

Vitro Assay ◽

Angiogenesis Assay

Objective: To determine the direct effect of pravastatin on angiogenesis and to study the interaction between pravastatin and maternal sera from women with early- or late-onset pre-eclampsia (PE), intrauterine growth restriction, or healthy pregnancy. Methods: We collected 5 maternal serum samples from each group. The effect of pravastatin on angiogenesis was assessed with and without maternal sera by quantifying tubule formation in a human-based in vitro assay. Pravastatin was added at 20, 1,000, and 8,000 ng/mL concentrations. Concentrations of angiogenic and inflammatory biomarkers in serum and in test medium after supplementation of serum alone and with pravastatin (1,000 ng/mL) were measured. Results: Therapeutic concentration of pravastatin (20 ng/mL) did not have significant direct effect on angiogenesis, but the highest concentrations inhibited angiogenesis. Pravastatin did not change the levels of biomarkers in the test media. There were no changes in angiogenesis when therapeutic dose of pravastatin was added with maternal sera, but there was a trend to wide individual variation towards enhanced angiogenesis, particularly in the early-onset PE group. Conclusions: At therapeutic concentration, pravastatin alone or with maternal sera has no significant effect on angiogenesis, but at high concentrations the effect seems to be anti-angiogenic estimated by in vitro assay.

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