scholarly journals Development of an oxidative stress in vitro assay in zebrafish (Danio rerio) cell lines

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sebastian Lungu-Mitea ◽  
Agneta Oskarsson ◽  
Johan Lundqvist
Keyword(s):  
Oxidative Stress ◽  
Cell Lines ◽  
Danio Rerio ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Chemical Constituents of Equisetum Debile and their Cytotoxic Activity

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Tran Huy Thai ◽  
Nguyen Quang Hung ◽  
Chau Van Minh ◽  
Nguyen Xuan Cuong ◽  
Pham Hai Yen ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cancer ◽  
Chemical Constituents ◽  
In Vitro Assay ◽  
The Other ◽  
Human Cancer Cell ◽  
Vitro Assay ◽  
Human Cancer Cell Lines ◽  
Aerial Parts

A new phenyl glycoside, equisetumoside D (1), was isolated from the aerial parts of Equisetum debile, along with equisetumoside B (2), dehydrovomifoliol (3), corchoionoside C (4), (-)-isolariciresinol-3a-O-β-D-glucopyranoside (5), and kaempferol 3-O-sophoroside-7-O-β-D-glucopyranoside (6). Their structures were elucidated by NMR spectroscopic and MS experiments. Compound 1 was found to be cytotoxic against both tested human cancer cell lines, hepatocellular carcinoma (Hep-G2, IC50: 1.12 μg/mL) and rhabdosarcoma (RD, IC50: 0.25 μg/mL), while the other compounds showed no activity against these cell lines by in vitro assay.

Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion

2011 ◽  
Vol 21 (S1) ◽  
pp. 52-62 ◽  
Author(s):  
Nicolas ATRUX-TALLAU ◽  
Sylvie CALLEJON ◽  
Camille MIGDAL ◽  
Karine PADOIS ◽  
Valérie BERTHOLLE ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Particle-induced cell migration assay (PICMA): A new in vitro assay for inflammatory particle effects based on permanent cell lines

2015 ◽  
Vol 29 (5) ◽  
pp. 997-1005 ◽  
Author(s):  
Götz A. Westphal ◽  
Isabell Schremmer ◽  
Alexander Rostek ◽  
Kateryna Loza ◽  
Nina Rosenkranz ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Lines ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Permanent Cell

An In Vitro Assay To Evaluate Competitive Exclusion Products for Poultry

2002 ◽  
Vol 65 (5) ◽  
pp. 746-751 ◽  
Author(s):  
R. DOUG WAGNER ◽  
MICHAEL HOLLAND ◽  
CARL E. CERNIGLIA
Keyword(s):  
Escherichia Coli ◽  
Cell Lines ◽  
Competitive Exclusion ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
In Vivo Testing ◽  
Commercial Poultry ◽  
Bacterial Mixtures

An in vitro assay was developed to measure the ability of competitive exclusion (CE) bacteria to protect Caco-2 and CRL-2117 epithelial cells from invasion by Salmonella Typhimurium. The proposed assay is needed to expedite the development of defined-flora CE products. The average significantly protective concentration of the commercial poultry-specific CE product Preempt was 4.05 log CFU/6.41 log human Caco-2 cells and 3.71 log CFU/6.89 log CFU chicken CRL-2117 cells. Enterococcus faecalis isolated from Preempt protected CRL-2117 cells, Escherichia coli isolates protected Caco-2 cells, Lactococcus lactis and Bacteroides distasonis isolates protected both cell lines, and three species of Lactobacillus isolates failed to protect either cell line. A defined mixture of 29 strains of bacteria similar to the constituents of Preempt protected both cell lines from Salmonella invasion at a concentration of 7.83 log CFU. The constituents of the defined CE culture were separated into mixtures of obligate (8.42 log CFU) and facultative (8.49 log CFU) anaerobes, which both protected the cell lines, suggesting that both types of bacteria were equally protective. Although not a substitute for in vivo testing, the in vitro CE assay is a rapid technique for the evaluation of bacterial mixtures for potential CE products.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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