scholarly journals Determinação da atividade antioxidante das matrizes vegetais Capsicum chinense, Capsicum frutescens e Tripodanthus acutifolius

2019 ◽  
Vol 9 (2) ◽  
pp. 98-105
Author(s):  
Silvio Augusto Ortolan ◽  
Vanessa Caroline Hermes ◽  
Pâmela Silveira Pedroso ◽  
Valéria Louzada Leal ◽  
Bruna Tischer ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Capsicum Frutescens ◽  
Capsicum Chinense ◽  
Santa Cruz

Radicais livres podem gerar estresse oxidativo e causar danos celulares. O uso de antioxidantes naturais demonstram ser um importante mecanismo para retardar ou inibir esse processo de oxidação. Matrizes vegetais dos gêneros Capsicum e Tripodanthus apresentam metabólitos secundários com atividade antioxidante relatada. Foi avaliada a atividade antioxidante de oleoresinas das pimentas Capsicum chinense e Capsicum frutescens, e do extrato bruto da espécie Tripodanthus acutifolius, através do ensaio Oxygen Radical Absorbance Capacity (ORAC). As oleoresinas de C. chinense e C. frutescens apresentaram valores de 550,210 µmol TE/g amostra e 603,746 µmol TE/g amostra, respectivamente. A espécie T. acutifolius apresentou valores aproximadamente dez vezes superiores (5277,764 µmol TE/g amostra) às oleoresinas. Observou-se uma relação direta da presença de compostos fenólicos com a atividade antioxidante. O ensaio realizado pelo grupo de pesquisas gerou um procedimento operacional interno que pode ser seguido em novas pesquisas de interesse com matrizes vegetais, aprovado para utilização no Parque Científico e Tecnológico Regional da Universidade de Santa Cruz do Sul (TecnoUnisc).

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

scholarly journals Determination of the Antioxidative Activities of Herbs Harvested in Japan by Oxygen Radical Absorbance Capacity Methods

2016 ◽  
Vol 22 (2) ◽  
pp. 301-305
Author(s):  
Manabu Wakagi ◽  
Yuuki Taguchi ◽  
Jun Watanabe ◽  
Tasuku Ogita ◽  
Masao Goto ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

scholarly journals Eficiencia de uso de nutrientes en ají tabasco (Capsicum frutescens L.) y habanero (Capsicum chínense Jacq)

2016 ◽  
Vol 7 (2) ◽  
pp. 121 ◽  
Author(s):  
María Del Pilar Romero-Lozada ◽  
Cristian Felipe Enciso Murillo ◽  
Sandra Marcela Sandra Marcela Garcia ◽  
Juan José Wagner Guerrero ◽  
Yina Jazbleidi Puentes-Páramo ◽  
...  
Keyword(s):  
Capsicum Frutescens ◽  
Capsicum Chinense

<p>El manejo adecuado de la nutrición de un cultivo implica hacer un uso eficiente de los nutrientes. Por tanto, esta investigación tuvo como objetivo determinar la eficiencia agronómica (EA) y de recuperación del fertilizante (ERF) para nitrógeno, fósforo y potasio en ají habanero y ají tabasco y su influencia en el rendimiento. El diseño experimental consistió en bloques completos al azar con cuatro tratamientos y cuatro repeticiones.  Los tratamientos fueron T1: Testigo, T2: fertilización según el programa que maneja el Centro Experimental de la Universidad Nacional de Colombia (CEUNP) para N-P-K, T3: programa de fertilización de CEUNP + 50% y el T4: programa de fertilización de CEUNP + 100%. Para ají tabasco (<em>C. frutescens </em>L<em>.</em>) y habanero (<em>C. chinense </em>J.) el análisis de varianza dio diferencias altamente significativas (p&lt;0,001) por efecto de los tratamientos tanto para la eficiencia agronómica (EA) como de recuperación del fertilizante (ERF). Los resultados muestran que los mayores valores de EA y ERF para N-P-K en tabasco  se presentaron en el tratamiento T4, y en habanero se presentaron en el tratamiento T3, excepto para la ERF de fósforo.  Así, estos permiten observar que ají tabasco presenta bajas eficiencias agronómicas y de recuperación de nutrientes con respecto al tipo habanero. Los mejores rendimientos de tabasco y habanero se presentan en el T4 y los menores en el T1, lo que sugiere una clara influencia del genotipo.</p>

Role of alkoxyl radicals on the fluorescein-based ORAC (Oxygen Radical Absorbance Capacity) assay

2014 ◽  
Vol 75 ◽  
pp. S38 ◽  
Author(s):  
E Dorta ◽  
E Atala ◽  
A Aspee ◽  
H Speisky ◽  
E Lissi ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

scholarly journals Chemical Constituents and Antioxidant Activity of Ficus callosa

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Phan Van Kiem ◽  
Nguyen Xuan Cuong ◽  
Nguyen Xuan Nhiem ◽  
Dan Thi Thuy Hang ◽  
Nguyen Hoai Nam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Chemical Evidence

One new megastigmane glycoside, ficalloside (1), and eleven known compounds, were isolated from methanol extract of Ficus callosa leaves by repeated column chromatography. Their structures were established on the basis of spectral and chemical evidence. The antioxidant activities of these compounds were measured using the oxygen radical absorbance capacity (ORAC) assay. Compound 8 exhibited potent antioxidant activity of 10.6 μM trolox equivalents at the concentration of 2 μM. At this concentration, compounds 4-7 and 9-12 showed significant antioxidant activity with ranging of 2.1~6.1 μM trolox equivalents.

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