High dietary concentrations of methionine reduce the selenium content, glutathione peroxidase activity and oxidative stability of chicken meat

Three experiments (EXP) were conducted using two hundred seventy male chicks Ross 308 in each (90 cockerels per treatment; 3 replications, 30 chickens per pen) for 42, 38 and 35 days. The basal diets (treatment 1) for three EXPs contained the identical ingredients, and the concentration of selenium (Se), methionine (Met) and total sulphur amino acids (TSAA) in the control diet was 0.11 mg/kg, 5.65 g per kg and 9.25 g/kg, respectively. Chicks in dietary treatment 2 were fed a basal diet supplemented with 0.3 mg/kg of Se (EXP 1, 2 and 3) and in dietary treatment 3 they were fed a basal diet with the addition of 0.3 mg/kg of Se and 1 g/kg (EXP 1 and 2) or 2.5 g/kg of dl-methionine (EXP 3). In EXP 1, sodium selenite and in EXP 2 and 3 Se-enriched yeast were used as sources of supplemental selenium, respectively. The results indicated that the addition of Se or Se and Met into the diet of broilers did not significantly affect the final live weight of chickens and the feed consumption. Moreover, the concentration of Met (P = 0.004), Cys (P = 0.01) and tyrosine (P < 0.001) in breast muscle increased with an increase in dietary Met content, and the isoleucine concentration decreased (P < 0.001). Moreover, the addition of inorganic and organic sources of Se increased the Se content of breast meat (P < 0.001). On the other hand, the addition of Met decreased the concentration of Se (P < 0.001) in breast meat and reduced glutathione peroxidase activity and oxidative stability of raw breast muscle (P = 0.019, P < 0.001) and breast meat stored for 3 days (P = 0.016, P = 0.006) in EXP 2 and 3.

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Effect of dietary selenium sources on growth performance, breast muscle selenium, glutathione peroxidase activity and oxidative stability in broilers

Czech Journal of Animal Science ◽

10.17221/361-cjas ◽

2008 ◽

Vol 53 (No. 6) ◽

pp. 265-269 ◽

Cited By ~ 30

Author(s):

G. Dlouhá ◽

S. Ševčíková ◽

A. Dokoupilová ◽

L. Zita ◽

J. Heindl ◽

...

Keyword(s):

Glutathione Peroxidase ◽

Oxidative Stability ◽

Growth Performance ◽

Sodium Selenite ◽

Selenium Concentration ◽

Basal Diet ◽

Breast Muscle ◽

Dietary Sodium ◽

Breast Meat ◽

Dietary Treatments

This study examined the effects of supplementation of dietary sodium selenite and sodium enriched alga Chlorella on growth performance, selenium concentration in breast meat and excreta, activity of glutathione peroxidase in meat, and oxidative stability of meat in broilers. Sexed broiler cockerels Ross 308 were allotted to 3 dietary treatments, each comprising 100 chickens. The basal diet was supplemented with 0 (control) or 0.3 mg/kg Se from sodium selenite (SS) or Se-Chlorella (SCH). Dietary supplementation with SCH increased (P < 0.05) body weight. The breast muscle Se concentration was increased (P < 0.05) by SCH (0.70 mg/kg DM; 0.36 mg/kg DM in control) supplementation, but not (P > 0.05) by SS (0.49 mg/kg DM) supplementation. The concentration of Se in excreta was highest in the SS group. The activity of GSH-Px in breast meat was significant P < 0.05) in all treatments (0.16 U/g in control, 0.30 U/g in SS and 0.23 U/g in SCH group). The inclusion of SCH in the diet enhanced the oxidative stability of meat expressed as reduced malondialdehyde (MDA) values in breast meat after 0; 3 and 5 days storage in refrigerator at 3 to 5°C.

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Comparative effects of selenium supplementation on the plasma selenium concentration and erythrocyte glutathione peroxidase activity in cattle and camels

Animal Science ◽

10.1017/s1357729800032872 ◽

1998 ◽

Vol 67 (3) ◽

pp. 461-466 ◽

Cited By ~ 15

Author(s):

M. Bengoumi ◽

A. K. Essamadi ◽

J. C. Tressol ◽

J. P. Chacornac ◽

B. Faye

Keyword(s):

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Selenium Concentration ◽

Basal Diet ◽

Glutathione Peroxidase Activity ◽

Plasma Selenium ◽

Selenium Metabolism ◽

Mineral Supplementation ◽

Very High ◽

Plasma Selenium Concentration

AbstractAt an experimental farm, five camels and five cows were each given a similar basal diet for 6 months. They received oral trace element supplementation for 3 months (day 22 to 112) which included zinc, copper, selenium, manganese, iodine and cobalt and corresponded to twice the daily requirement generally recommended for cows. Plasma selenium concentrations were significantly lower in the camels (20 (s.e. 2) mg/l) compared with the cows (33 (s.e. 2) μg/l). The mineral supplementation induced a large increase in the plasma selenium concentration in the camels which reached 200 (s.e. 35) fig/l. In the cows, the increase was much smaller and did not go beyond 65 (s.e. 8) μg/l. Before supplementation the red blood cell glutathione peroxidase activity was similar in the camel and the cow varying between 4000 and 6000 IU per 100 g haemoglobin. In both species, this activity increased with mineral supplementation and remained very high even when mineral supplementation was stopped. The results suggested that selenium metabolism in camels is different from that in the cows.

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Evaluation of productive performance of broiler in response to Koroch (Pongamia pinnata) cake feeding

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v3i2.29357 ◽

2016 ◽

Vol 3 (2) ◽

pp. 315-321

Author(s):

Masuma Habib ◽

Abu Jafur Md Ferdaus ◽

Md Touhidul Islam ◽

Begum Mansura Hassin ◽

Md Shawkat Ali

Keyword(s):

Live Weight ◽

Dietary Treatment ◽

Basal Diet ◽

Pongamia Pinnata ◽

Feed Consumption ◽

Broiler Chicks ◽

Growth Depression ◽

Meat Yield ◽

Treatment Groups ◽

Seed Cake

The study was conducted for a period of 28 days to investigate the response of broiler to the inclusion of de-oiled koroch (Pongamia Pinnata) seed cake in the diet of broiler. A total of 192 day-old broiler chicks were individually weighed and randomly allocated to 4 dietary treatment groups having 4 replications of 12 chicks each, in a completely randomized design. Broilers under treatment 1 received a basal diet containing no koroch seed cake, considered as control; in treatment 2, 3 and 4, broilers were fed on basal diet containing 2%, 4% and 6% koroch seed cake, respectively. All productive performances (live weight, live weight gain, feed consumption and feed efficiency) of broiler fed on koroch seed cake were significantly (P<0.01) depressed compared to the control. The degree of depression was increased with the increasing level of koroch seed cake in the diet at all ages of broiler. The meat yield parameters showed a non-significant (P>0.05) effect except for the percentage of dressing yield, breast, liver and gizzard weight for the broilers in all treatment groups. Broilers fed on diet containing 6% koroch seed cake yielded the lowest dressed weight in the treatment groups. Inclusion of 2% koroch seed cake resulted in higher breast meat yield compared to any other level of koroch cake inclusion in the diet (P<0.01). However, liver and gizzard weight were increased significantly (P<0.05) for incorporation of de-oiled koroch seed cake in the diet at all levels (2%, 4% or 6%). On the basis of these results it is concluded that feeding de-oiled koroch cake had no positive effect on growth response and meat yield characteristics of broiler. Therefore, it is suggested that the koroch seed cake may contain anti-nutritional factor(s), which seemed to be associated with growth depression in broilers. Further research is warranted to alleviate the potential toxic effect of koroch seed cake on broiler performances.Res. Agric., Livest. Fish.3(2): 315-321, August 2016

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Dietary fish oil replacement with palm or poultry oil increases fillet oxidative stability and decreases liver glutathione peroxidase activity in barramundi (Lates calcarifer)

Fish Physiology and Biochemistry ◽

10.1007/s10695-013-9815-5 ◽

2013 ◽

Vol 39 (6) ◽

pp. 1631-1640 ◽

Cited By ~ 5

Author(s):

Wan A. R. Wan Ahmad ◽

David A. J. Stone ◽

Kathryn A. Schuller

Keyword(s):

Glutathione Peroxidase ◽

Fish Oil ◽

Oxidative Stability ◽

Peroxidase Activity ◽

Glutathione Peroxidase Activity ◽

Lates Calcarifer ◽

Fish Oil Replacement ◽

Liver Glutathione ◽

Dietary Fish

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Low Selenium Status in the Elderly Influences Thyroid Hormones

Clinical Science ◽

10.1042/cs0890637 ◽

1995 ◽

Vol 89 (6) ◽

pp. 637-642 ◽

Cited By ~ 64

Author(s):

Oliviero Olivieri ◽

Domenico Girelli ◽

Margherita Azzini ◽

Anna Maria Stanzial ◽

Carla Russo ◽

...

Keyword(s):

Glutathione Peroxidase ◽

Thyroid Hormones ◽

Peroxidase Activity ◽

Controlled Trial ◽

The Elderly ◽

Serum Selenium ◽

Selenium Status ◽

Glutathione Peroxidase Activity ◽

Double Blind ◽

Double Blind Placebo

1. Iodothyronine 5′-deiodinase, which is mainly responsible for peripheral triiodothyronine (T3) production, has recently been demonstrated to be a selenium-containing enzyme. In the elderly, reduced peripheral conversion of thyroxine (T4) to T3 and overt hypothyroidism are frequently observed. 2. We measured serum selenium and erythrocyte glutathione peroxidase (as indices of selenium status), thyroid hormones and thyroid-stimulating hormone in 109 healthy euthyroid subjects (52 women, 57 men), carefully selected to exclude abnormally low thyroid hormone levels induced by acute or chronic diseases or calorie restriction. The subjects were subdivided into three age groups. To avoid conditions of undernutrition or malnutrition, dietary records were obtained for a sample of 24 subjects, randomly selected and representative of the whole population for age and sex. 3. In order to properly assess the influence of selenium status on iodothyronine 5′-deiodinase type I activity, a double-blind placebo-controlled trial was also carried out on 36 elderly subjects, resident at a privately owned nursing home. 4. In the free-living population, a progressive reduction of the T3/T4 ratio (due to increased T4 levels) and of selenium and erythrocyte glutathione peroxidase activity was observed with advancing age. A highly significant linear correlation between T4, T3/T4 and selenium was observed in the population as a whole (for T4, R = −0.312, P < 0.002; for T3/T4 ratio, R = 0.32, P < 0.01) and in older subjects (for T4, R = −0.40, P < 0.05; for T3/T4 ratio, R = 0.54, P < 0.002). 5. The main result of the double-blind placebo-controlled trial was a significant improvement of selenium indices and a decrease in the T4 level in selenium-treated subjects; serum selenium, erythrocyte glutathione peroxidase activity and thyroid hormones did not change in placebo-treated subjects. 6. We concluded that selenium status influences thyroid hormones in the elderly, mainly modulating T4 levels.

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Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers

Orphanet Journal of Rare Diseases ◽

10.1186/s13023-021-01732-5 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Itana Gomes Alves Andrade ◽

Fabíola Isabel Suano-Souza ◽

Fernando Luiz Affonso Fonseca ◽

Carolina Sanchez Aranda Lago ◽

Roseli Oselka Saccardo Sarni

Keyword(s):

Oxidative Stress ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Oxidized Ldl ◽

Reference Value ◽

Glutathione Peroxidase Activity ◽

Oxidative Stress Biomarkers ◽

Apo B ◽

Lipid Status ◽

And Oxidative Stress

Abstract Introduction Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress. Objective To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers. Methods This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers. Results Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups. Conclusion In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.

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The distribution, induction and isoenzyme profile of glutathione S-transferase and glutathione peroxidase in isolated rat liver parenchymal, Kupffer and endothelial cells

Biochemical Journal ◽

10.1042/bj2640737 ◽

1989 ◽

Vol 264 (3) ◽

pp. 737-744 ◽

Cited By ~ 18

Author(s):

P Steinberg ◽

H Schramm ◽

L Schladt ◽

L W Robertson ◽

H Thomas ◽

...

Keyword(s):

Endothelial Cells ◽

Glutathione Peroxidase ◽

Rat Liver ◽

Peroxidase Activity ◽

Cell Types ◽

Transferase Activity ◽

Glutathione Peroxidase Activity ◽

Glutathione S Transferase ◽

Liver Parenchymal ◽

Parenchymal Cells

The distribution and inducibility of cytosolic glutathione S-transferase (EC 2.5.1.18) and glutathione peroxidase (EC 1.11.1.19) activities in rat liver parenchymal, Kupffer and endothelial cells were studied. In untreated rats glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene and 4-hydroxynon-2-trans-enal as substrates was 1.7-2.2-fold higher in parenchymal cells than in Kupffer and endothelial cells, whereas total, selenium-dependent and non-selenium-dependent glutathione peroxidase activities were similar in all three cell types. Glutathione S-transferase isoenzymes in parenchymal and non-parenchymal cells isolated from untreated rats were separated by chromatofocusing in an f.p.l.c. system: all glutathione S-transferase isoenzymes observed in the sinusoidal lining cells were also detected in the parenchymal cells, whereas Kupffer and endothelial cells lacked several glutathione S-transferase isoenzymes present in parenchymal cells. At 5 days after administration of Arocolor 1254 glutathione S-transferase activity was only enhanced in parenchymal cells; furthermore, selenium-dependent glutathione peroxidase activity decreased in parenchymal and non-parenchymal cells. At 13 days after a single injection of Aroclor 1254 a strong induction of glutathione S-transferase had taken place in all three cell types, whereas selenium-dependent glutathione peroxidase activity remained unchanged (endothelial cells) or was depressed (parenchymal and Kupffer cells). Hence these results clearly establish that glutathione S-transferase and glutathione peroxidase are differentially regulated in rat liver parenchymal as well as non-parenchymal cells. The presence of glutathione peroxidase and several glutathione S-transferase isoenzymes capable of detoxifying a variety of compounds in Kupffer and endothelial cells might be crucial to protect the liver from damage by potentially hepatotoxic substances.

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