scholarly journals Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.)

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 84-88 ◽  
Author(s):  
L. Klčová ◽  
M. Gubišová
Keyword(s):  
Culture Medium ◽  
Nodal Segments ◽  
Induction Medium ◽  
Ms Medium ◽  
In Vitro Techniques ◽  
In Vitro Methods ◽  
Regenerated Plants ◽  
Plant Maintenance

In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H<sub>2</sub>SO<sub>4</sub> and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not support multiplication and growth. In the basal MS medium rooted 72.5% (gene resource 62) or 42.5% (gene resource 28) of shoots. The rooting of gene resource 28 was increased to 63% by the addition of indolylbutyric acid to the culture medium. The regenerated plants were successfully transferred to the soil. This protocol can be used to rescue gene resources of this species. &nbsp; &nbsp;

scholarly journals In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

2017 ◽  
pp. 50-55
Author(s):  
Khalid Lagram ◽  
Mohamed Ben El Caid ◽  
Souad El Aaouam ◽  
Mohamed Lachheb ◽  
Abdelhamid El Mousadik ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Crocus Sativus ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Male Sterile ◽  
Indirect Organogenesis ◽  
Crocus Sativus L

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

scholarly journals Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants

2010 ◽  
Vol 32 (2) ◽  
pp. 612-617 ◽  
Author(s):  
Fernanda Vidigal Duarte Souza ◽  
Ana Maria Mascarenhas Eloy Canto ◽  
Antônio da Silva Souza ◽  
Maria Angélica Pereira de Carvalho Costa
Keyword(s):  
Gibberellic Acid ◽  
Culture Medium ◽  
Residual Effect ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Proliferation Medium ◽  
Regenerated Plants ◽  
Subsequent Regeneration ◽  
Previous Step

This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae)

2010 ◽  
Vol 58 (3) ◽  
pp. 198 ◽  
Author(s):  
Aastha Sahai ◽  
Anwar Shahzad ◽  
Shiwali Sharma
Keyword(s):  
Somatic Embryogenesis ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Tylophora Indica ◽  
Regenerated Plants ◽  
Maturation Medium ◽  
Half Strength ◽  
One Year

This paper reports an efficient regeneration protocol through parallel organogenic and embryogenic pathways from green root segments (GRSs) of Tylophora indica (Burm.f) Merrill. GRSs explants from one year old in vitro cultures were cultured on Murashige and Skoog (MS) medium containing various cytokinins. Five µmol/L of 6-benzyladenine (BA) was most responsive for organogenesis in 1.5 cm long GRSs. Repeated subculture on medium containing both BA (5 µmol/L) and 1-naphthleneacetic acid (NAA) (0.1 µmol/L) promoted multiplication and proliferation of direct shoot buds (46.80 ± 0.96) and callus mediated somatic embryogenesis (18.07 ± 0.33). Germinated embryos isolated from callus were transferred onto maturation medium consisting of half-strength MS medium either devoid of plant growth regulators (PGRs) or with various concentrations of gibberellic acid (GA). Microshoots were excised during subculture and transferred onto root induction medium, thus ensuring a continuous supply of germplasm. Morphogenic variations were noticed in types of roots induced on various auxins. Regenerated plantlets and emblings hardened best on vermiculite with a survival rate of 90% and 70% respectively. However, the emblings were healthier in comparison to the regenerated plants. Histological analysis showed the origin and development of organogenesis.

scholarly journals In-vitro callus induction and multiplication of inter-nodal explants in plants Dicoma tomentosa and Alhagi maurorum

2019 ◽  
Vol 9 (4-A) ◽  
pp. 212-219 ◽  
Author(s):  
Shilpa Dhaniya ◽  
Suman Kumari Parihar
Keyword(s):  
Medicinal Plants ◽  
Culture Medium ◽  
Raw Material ◽  
Nodal Segments ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Primary Metabolites ◽  
Shoot Initiation ◽  
Rooting Medium

Dicoma tomentosa and Alhagi maurorum are the two medicinal plants with fast in-vitro growth. Both the plants have high economic values. Both the plants were investigated on nodal segments and on leaves. The plants were cultured in five different conditions of medium ranging from MS1- MS5. The hormones were used in these mediums in different concentrations. BAP, NAA, Kinetin, and 2,4 D were use. The MS medium in combination with BAP (2.0 and 2.0mg/ml) with NAA 0.1 mg/ml with kinetin 0.25 mg/ml with 2-4 D were taken, where BAP 1 mg/ml with 2 mg/ml of NAA, BAP 2 mg/ml with 0.5 mg/ml of NAA showed better results with callus growth and root-shoot initiation. The best rooting medium found was MS medium supplemented with IAA and IBA 0.5mg/ each. The culture medium was used in different concentrations for estimation of primary metabolites. Maximum protein and lipid percentage were noticed in leaves of both the plants. It can be concluded that both the studied plants have high medicinal importance and can be used as raw material for industry. Keywords: - Dicoma tomentosa; Alhagi maurorum; Plant hormones; MS media.

scholarly journals Micropropagation of Juvenile and Adult Flowering Ash

1992 ◽  
Vol 117 (2) ◽  
pp. 346-350 ◽  
Author(s):  
Isabel Arrillaga ◽  
Victoria Lerma ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Nodal Segments ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Shoot Apices ◽  
Regenerated Plants ◽  
Adult Trees

A protocol for in vitro propagation in flowering ash (Fraxinus ornus L.) has been developed. Shoot apices or nodal segments from aseptically grown seedings or shoot apices from adult trees were used as initial explants. Highest shoot multiplication rates were obtained when the explants were cultured for 30 days in liquid Rugini induction medium supplemented with BA followed by 30 days on solidified Rugini multiplication medium without growth regulators. Regenerated shoots were rooted on Heller medium containing auxins alone or in combination with BA. Rooting percentages up to 71% (juvenile material) or 50% (adult material) were obtained in the presence of NAA and BA, and were not improved by treating the basal end of the shoots with concentrated NAA solutions. Following conventional procedures, regenerated plants were transferred to soil with more than 80% success. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

2016 ◽  
Vol 4 (1) ◽  
pp. 61-69 ◽  
Author(s):  
S Yesmin ◽  
A Hashem ◽  
MS Islam
Keyword(s):  
Morphological Variation ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

scholarly journals Multiplicação de anador (Justicia pectoralis) in vitro

2016 ◽  
Vol 11 (3) ◽  
pp. 159 ◽  
Author(s):  
Rômulo Magno Oliveira Freitas ◽  
Narjara Walessa Nogueira ◽  
Sidney Carlos Praxedes
Keyword(s):  
Plant Material ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Statistical Design ◽  
The Other ◽  
Nodal Segments ◽  
Ms Medium ◽  
Number Of Leaves

<p>O trabalho teve como objetivo desenvolver um protocolo de micropropagação de segmentos nodais de anador (<em>Justicia pectoralis</em>). Para isso foram realizados dois experimentos. O delineamento estatístico utilizado foi o inteiramente casualizado, com 15 repetições. Os segmentos de <em>J. pectoralis</em>, após desinfestados, foram cultivados em meio MS durante 30 dias. No primeiro experimento, esse material foi repicado em três meios de cultura (MS, WPM e B5) e após 77 dias foram avaliados comprimento de plântula, número de raízes, número de folhas e o número de segmentos nodais. Para o segundo experimento foram testadas duas citocininas (BAP e Cinetina) nas seguintes dosagens 0,0; 0,5; 5,0 e 20,0 mM. Aos 60 dias após a repicagem foram avaliadas as seguintes características: números de folhas, número de raízes e número de explantes por planta. O meio MS foi o que apresentou maior comprimento de plântula. As demais variáveis não diferiram entre os meios utilizados. Por isso o meio MS foi utilizado para o segundo experimento onde se verificou que a utilização de BAP proporcionou maior número de folhas e de explantes quando submetido à concentração de 20 mM. Dessa forma, para multiplicação de seg <em>Justicia pectoralis</em>, recomenda-se a utilização de meio MS com adição de 20mM de BAP.</p><p align="center"><strong><em>In vitro propagation of </em></strong><em>Justicia pectoralis<strong></strong></em></p><p><strong>Abstract</strong><strong>: </strong>The study aimed to establish a micropropagation protocol for <em>Justicia pectoralis</em> nodal segments. Two experiments were conducted. The statistical design was the completely randomized with 15 repetitions. After disinfestation, the segments of <em>J. pectoralis</em> were inoculated in the MS culture medium for 30 days. In the first experiment, the plant material was transferred to three culture media (MS, WPM and B5). The length of seedlings, number of roots, number of leaves, and number of nodal segments were evaluated at 77 days after transferring. In the second experiment two cytokinins (BAP and Kinetin) were tested in the following concentrations: 0.0; 0.5; 5.0 and 20.0 mM. At 60 days after transplanting the number of leaves, number of roots and number of explants per plant were evaluated. The MS medium induced the highest length of seedlings, but there was no effect for the other variables. Therefore, this medium was used for the second experiment, when it was found that BAP induced a larger number of leaves and explants when applied at 20 mM. Therefore, for multiplying <em>J. pectoralis</em> nodal segments we recommend the use of MS medium with 20 mM BAP.</p>

scholarly journals Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh

2013 ◽  
Vol 47 (4) ◽  
pp. 373-378
Author(s):  
AKMS Hassan ◽  
CK Roy ◽  
R Sultana ◽  
R Khatun
Keyword(s):  
Plant Regeneration ◽  
Leaf Shape ◽  
Basal Medium ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
Paederia Foetida

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obtained when the callus was sub cultured on MS medium with 0.5 mg/L BAP. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/L IBA. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth. Bangladesh J. Sci. Ind. Res. 47(4), 373-378, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14066

scholarly journals Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions

2010 ◽  
Vol 58 (1) ◽  
pp. 147-152
Author(s):  
Marcel Raček ◽  
Helena Lichtnerová ◽  
Marta Dragúňová ◽  
Alena Gajdošová ◽  
Anna Jakábová
Keyword(s):  
Hormonal Regulation ◽  
Culture Medium ◽  
Shoot Multiplication ◽  
Positive Influence ◽  
Nodal Segments ◽  
Basic Medium ◽  
Ms Medium ◽  
Optimal Protocol ◽  
Mother Plants

Experimental works were focused on creation of optimal protocol forAcer davidiissp.grosserishoots multiplication. As the basic medium we used MS (Murashige α Skoog) culture medium enriched by cytokinins TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) in different concentrations. There were used single nodal segments from four years old mother plants in experiments. Experiments were established in the middle of June in the phase of rapid growth of shoots. Cultivation conditions were: 21°C, light intensity 40 μmol . m−2. s−1, period of light 16 hours during the day. The reactions of explants were evaluated after six and twelve month. The results were statistically analysed. The explants ofAcer davidiissp.grosserireacted differently on hormonal regulation. The lowest multiplication activity of explants was found out on MS culture medium enriched by 2,6 μM BAP. The average shoot multiplication on MS + 0,5 μM TDZ culture medium was 1,2. Multiplication 1,49 shoot per explants was found out on MS + 2,6 μM BAP + 0,5 μM TDZ. On the basic MS medium 1,35 shoots per explants multiplicated. In spite of positive influence of application of TDZ and BAP on shoot multiplication the results compared with multiplication on MS medium were statistically insignificant.

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