scholarly journals In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

2017 ◽  
pp. 50-55
Author(s):  
Khalid Lagram ◽  
Mohamed Ben El Caid ◽  
Souad El Aaouam ◽  
Mohamed Lachheb ◽  
Abdelhamid El Mousadik ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Crocus Sativus ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Male Sterile ◽  
Indirect Organogenesis ◽  
Crocus Sativus L

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

Rapid in vitro Propagation of Dodonaea viscosa (Sapindaceae) Through Axillary Bud Multiplication and Indirect Organogenesis

2015 ◽  
Vol 22 (1) ◽  
pp. 33-36
Author(s):  
A. Benniamin ◽  
G. Jothi ◽  
M. Sundari
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
High Rate ◽  
Axillary Bud ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Induction ◽  
Dodonaea Viscosa ◽  
Indirect Organogenesis

Protocols of axillary bud multiplication were established for Dodonaea viscosa (Sapindaceae). Murashig & Skoog’s medium with 0.2 mg/l BAP with 0.01 Naphthalene acetic acid induced high rate of shoot induction and an average of five shoots per node. Subsequent culture enhanced the number of shoots. Callus initiated from the basal cut end explants differentiated in to more than 10 shoots on MS Medium with 0.4mg/l Benzylaminopurine and 0.02mg/l Indole Butric Acid. Eighty per cent of the rooted shoots survived when transferred to greenhouse and subsequently to the field.

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (Vetiveria nemoralis A. Camus cv. Roiet)

2015 ◽  
Vol 804 ◽  
pp. 259-262
Author(s):  
Chonnikarn Khunchuay ◽  
Kanokporn Sompornpailin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Shoot Number ◽  
Induction Experiment

The optimum ratios of auxin and cytokinin are necessary for callus induction and plant regeneration. This ratio is a key function involving in the promoting cell division and proliferation in tissue culture. The axillary buds of in vitro plantlets fromVetiveria nemoralisA. Camuscv. Roiet were used as explants for the callus induction experiment. These explants were cultured on Murashige & Skoog (MS) medium [1] supplemented with various combinations of auxins and cytokinins. Under this experimental study, the highest frequency of callus induction was found on MS medium supplemented with 2 mgL-1α-naphthalene acetic acid (NAA) and 1 mgL-12-furanylmethyl-1H-purine-6-amine (kinetin) (62.5%). On the other hand the combination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) was toxicity to this explants. All culturing explants were dead and no calli appearance. The calli derived from each medium were transferred into the same regeneration medium (MS with 1 mgL-1NAA and 2 mgL-1BAP). After culturing on regeneration medium, calli induced from the highest callus induction medium have shown high frequencies of regeneration and also shoot number per callus (58.33% and 7.1 shoots).

scholarly journals Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.)

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 84-88 ◽  
Author(s):  
L. Klčová ◽  
M. Gubišová
Keyword(s):  
Culture Medium ◽  
Nodal Segments ◽  
Induction Medium ◽  
Ms Medium ◽  
In Vitro Techniques ◽  
In Vitro Methods ◽  
Regenerated Plants ◽  
Plant Maintenance

In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H<sub>2</sub>SO<sub>4</sub> and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not support multiplication and growth. In the basal MS medium rooted 72.5% (gene resource 62) or 42.5% (gene resource 28) of shoots. The rooting of gene resource 28 was increased to 63% by the addition of indolylbutyric acid to the culture medium. The regenerated plants were successfully transferred to the soil. This protocol can be used to rescue gene resources of this species. &nbsp; &nbsp;

In vitro shoots and micro-corms formation through indirect organogenesis of Moroccan saffron (Crocus sativus L.)

2017 ◽  
pp. 97-108
Author(s):  
K. Lagram ◽  
M. Ben El Caid ◽  
L.H. Atyane ◽  
L. Salaka ◽  
R. El Boullani ◽  
...  
Keyword(s):  
Crocus Sativus ◽  
Indirect Organogenesis ◽  
In Vitro Shoots ◽  
Crocus Sativus L

An effective protocol for improved regeneration capacity of Kabuli chickpeas

2012 ◽  
Vol 92 (6) ◽  
pp. 1057-1064 ◽  
Author(s):  
I. S. Yadav ◽  
N. P. Singh
Keyword(s):  
Shoot Regeneration ◽  
Culture Medium ◽  
Genetic Manipulation ◽  
In Vitro Regeneration ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regeneration Capacity ◽  
Regeneration System ◽  
Embryonic Axes

Yadav, I. S. and Singh, N. P. 2012. An effective protocol for improved regeneration capacity of Kabuli chickpeas. Can. J. Plant Sci. 92: 1057–1064. An efficient protocol for in vitro regeneration is essential for genetic manipulation and micro-propagation of important plant species. A direct shoot regeneration system has been optimized for Desi chickpeas, but an effective regeneration protocol is still needed for Kabuli chickpeas. An efficient regeneration protocol for Kabuli chickpeas was developed, using whole embryonic axes, an embryonic axes slice and cotyledonary node explants from two genotypes L550 and JGK-1. Depending upon chickpea genotype, type of explant and culture medium, percentage of shoot producing explants (frequency) and the number of shoots per explant (efficiency) varied from 10 to 83% and from 1 to 58, respectively. The shoot regeneration capacity (SRC=frequency×efficiency), which is an indicator of the effectiveness of the protocol, varied from 47 to 2508 shoots per 100 explants cultured. On average, SRC of L550 was 1.8 times higher than JGK-1. Murashige and Skoog's (MS) medium+B5 vitamins supplemented with 8.0 µM benzyl amino purine (BAP)+0.5 µM α- naphthalene acetic acid (NAA) and 0.1 M sucrose plus embryonic axes was found to be the most effective culture medium and type of explants, respectively. Half strength MS medium+2% sucrose supplemented with 4 µM NAA, 3µ M IAA or 4µM IAA produced a high rooting percentage in both chickpea genotypes. The regeneration process starting from explant preparation to establishment of a complete plant in soil took 105–110 d. This optimized regeneration method holds promise for facilitating the insertion of interested genes through genetic transformation for improvement of Kabuli chickpeas.

scholarly journals Effect of plant growth regulators on in vitro culture of pineapple (Ananas comosus L. Merr) MD2 variety

Food Research ◽  
2020 ◽  
Vol 4 (S5) ◽  
pp. 110-114
Author(s):  
S. Sulaiman ◽  
N.A. Yusuf ◽  
A. Awal
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Shoot Regeneration ◽  
Ananas Comosus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Short Period ◽  
Planting Materials ◽  
In Vitro Shoot Regeneration

Pineapples (Ananas comosus L. Merr) are fruits that belong to the Bromeliaceae family. Pineapple variety MD2 is one of the varieties that has gained a place in the market among pineapple farmers due to its high value and quality. However, it is difficult to meet the demand for planting materials using conventional propagation techniques. Hence, plant tissue culture technology is one of the methods that has been widely used in the agriculture industry that boosts up the production of pineapple planting materials within a short period and is cost-efficient. The objective of this study was to determine the effect of plant growth regulator concentration to in vitro culture of MD2 variety pineapple. In this study, the various concentrations of 6-Benzylaminopurine (BAP) and α-Naphthalene Acetic Acid (NAA) for in vitro culture of MD2 pineapple were studied. The plantlets were effectively initiated from MD2 pineapple crown on Murashige and Skoog (MS) basal salt containing 30.0 g/L sucrose, and 2.0 mg/L BAP in two months of culture. Next, the pineapple plantlet was subculture on shooting medium containing full strength solid Murashige and Skoog (MS) medium supplemented with vitamins with various concentration BAP (0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0 mg/L) and NAA (0, 1.0, and 2.0 mg/L). The result obtained showed that the solid MS medium added with 30.0 g/L sucrose, without any BAP and NAA (T1) had the highest in vitro shoot regeneration. Meanwhile, the solid MS medium with 30.0 g/L sucrose with 1.0 mg/L NAA (T1) recorded the highest plantlet height (cm). The mean value for in vitro shoot regeneration in T1 and plantlet height (cm) in T1 were 2.80 (±0.5) and 4.40 (±0.3). To conclude, less amount plant hormone regulator required to obtain the mass quantity of in vitro clonal pineapple that can help solve the problem of lack of plant material in the pineapple crop industry.

scholarly journals Micropropagation of Phalaenopsis amabilis var. ‘Manila’ by Leaves Obtained from in Vitro Culturing the Nodes of Flower Stalks

2016 ◽  
Vol 8 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Khosro BALILASHAKI ◽  
Masood GHASEMI GHEHSAREH
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Culture Medium ◽  
Global Market ◽  
Ms Medium ◽  
Current Experiment ◽  
Flower Stalk ◽  
The World

Orchids are one of the most popular plants in the world and among them the species of Phalaenopsis have the most sales on the global market. Because of its difficult propagation, micropropagation has been suggested recently. In the current study, the leaves obtained from in vitro culture of flower stalk nodes were used as explants and were cultured on MS medium with different concentrations of NAA, BA and TDZ. Protocorm-like bodies (PLBs) were produced and transferred to medium without growth regulators. Finally, the adaptation of plants was evaluated in a medium of cocopeat + coal (3:1 v/v) and another medium of cocopeat + charcoal + LECA (2:1:2 v/v). Results showed that the highest percentage of active samples was 100% which could regenerate the PLBs by being treated with 4 mg/l TDZ. The lowest active samples (60%) were those treated in the medium with 4 mg/l BAP + 0.5 mg/l NAA. The highest PLBs per explant (50.65) were obtained in the medium supplemented with 15 mg/l BAP + 3 mg/l NAA. Best acclimation (90%) of plants was obtained when medium of cocopeat + charcoal + LECA (2:1:2 v/v) was used. According to the results of the current experiment, the MS culture medium containing 15 mg/l BAP + 3 mg/l NAA was thereby considered as the best medium for Phalaenopsis micropropagation.

scholarly journals In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Hoehnea ◽  
2018 ◽  
Vol 45 (4) ◽  
pp. 663-668
Author(s):  
Daniel da Silva ◽  
Angela Maria Imakawa ◽  
Suely de Souza Costa ◽  
Paulo de Tarso Barbosa Sampaio
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Germination Rate ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Growth Room ◽  
Morphogenetic Responses

ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

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