Phytoplasma occurrence in apple trees in the Czech Republic

The presence of phytoplasmas in apple trees with proliferation symptoms, rubbery wood symptoms and no symptoms was determined by using polymerase chain reaction assays with primers amplifying phytoplasma 16S rRNA gene. Phytoplasmas were detected in all trees with proliferation symptoms. Positive tests for phytoplasma in the group of trees with rubbery wood symptoms and of those without symptoms revealed a relatively high incidence of latent phytoplasma infection. Using restriction fragment length polymorphism analysis, phytoplasma of the same identity – apple proliferation phytoplasma (subgroup 16SrX-A) – was recorded in all positively tested trees.  

Download Full-text

First Detection of Phytoplasmas in Rhododendron in the Czech Republic

Plant Disease ◽

10.1094/pdis.2004.88.8.906a ◽

2004 ◽

Vol 88 (8) ◽

pp. 906-906 ◽

Cited By ~ 2

Author(s):

J. Mertelik ◽

K. Kloudova ◽

P. Vanc ◽

V. Mokra ◽

J. Sediva ◽

...

Keyword(s):

Fragment Length Polymorphism ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

The Czech Republic ◽

First Finding ◽

Phytoplasma Infection ◽

Polymerase Chain ◽

Stolbur Phytoplasma ◽

Restriction Fragment Length

A 10-year-old plant of rhododendron (‘Cunningham's White’ PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infection was detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII group) as determined previously (2). To our knowledge, this is the first finding of stolbur-type phytoplasma in rhododendron worldwide. References: (1) R. Fialova et al. Plant Prot. Sci. 39(1):7, 2003. (2) I.-M Lee et al. Int. J. Syst. Bacteriol. 48:1153,1998.

Download Full-text

Sequence and polymerase chain reaction-restriction fragment length polymorphism analysis of the large subunit rRNA gene of bivalve: Simple and widely applicable technique for multiple species identification of bivalve larva

Fisheries Science ◽

10.1111/j.1444-2906.2004.00850.x ◽

2004 ◽

Vol 70 (4) ◽

pp. 629-637 ◽

Cited By ~ 15

Author(s):

Masatomi HOSOI ◽

Shoko HOSOI-TANABE ◽

Hideki SAWADA ◽

Masahiro UENO ◽

Haruhiko TOYOHARA ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Fragment Length Polymorphism ◽

Large Subunit ◽

Rrna Gene ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Polymerase Chain ◽

Multiple Species ◽

Large Subunit Rrna ◽

Restriction Fragment Length

Download Full-text

The Genetic Relationship Between Mycoplasma-like Organisms Causing Diseases in the Sudan and Different Continents Revealed by Polymerase Chain Reaction (PCR) Amplification of the 16S rRNA Gene Followed by Restriction Fragment Length Polymorphism Analysis

Journal of Phytopathology ◽

10.1111/j.1439-0434.1995.tb00193.x ◽

1995 ◽

Vol 143 (1) ◽

pp. 17-20 ◽

Cited By ~ 2

Author(s):

E. M. Saeed ◽

M. T. Cousin

Keyword(s):

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

Fragment Length Polymorphism ◽

Pcr Amplification ◽

Rrna Gene ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Polymerase Chain ◽

The 16S Rrna Gene ◽

Restriction Fragment Length

Download Full-text

Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of a 16S rRNA Gene Fragment for Authentication of Four Clam Species

Journal of Food Protection ◽

10.4315/0362-028x-65.4.692 ◽

2002 ◽

Vol 65 (4) ◽

pp. 692-695 ◽

Cited By ~ 12

Author(s):

ALICIA FERNÁNDEZ ◽

TERESA GARCÍA ◽

ISABEL GONZÁLEZ ◽

LUIS ASENSIO ◽

MIGUEL ÁNGEL RODRÍGUEZ ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

16S Rrna ◽

16S Rrna Gene ◽

Fragment Length Polymorphism ◽

Rrna Gene ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Polymerase Chain ◽

Restriction Fragment Length

Specific identification of four clam species, Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), Ruditapes philippinarum (Japanese carpet shell), and Venerupis rhomboides (yellow carpet shell), was achieved by polymerase chain reaction–restriction fragment length polymorphism analysis of a fragment of the mitochondrial 16S rRNA gene. Amplification of DNA isolated from the foot muscle produced fragments of 511 bp for V. pullastra, 523 bp for R. decussatus, 545 bp for R. philippinarum, and 502 bp for V. rhomboides. The restriction profiles obtained by agarose gel electrophoresis when amplicons were digested with endonucleases BsmAI and BsrI allowed unequivocal identification of the four clam species. This approach would be less costly, simpler, and quicker than conventional sequencing of polymerase chain reaction products followed by detailed comparison of individual sequences, especially when large numbers of samples need to be analyzed.

Download Full-text