scholarly journals Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

2012 ◽  
Vol 49 (No. 3) ◽  
pp. 65-69 ◽  
Author(s):  
G. Ozbey ◽  
A. Kilic ◽  
H. B Ertas ◽  
A. Muz
Keyword(s):  
Genetic Heterogeneity ◽  
Pasteurella Multocida ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mannheimia Haemolytica ◽  
Random Primer ◽  
Sheep And Goats ◽  
Distinct Band ◽  
Cattle And Sheep

n this study, 30, 15 and 1 strains of <i>Pasteurella multocida</i> and 9, 8 and 6 strains of <i>Mannheimia haemolytica</i> from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of <i>P. multocida</i> isolates from cattle and sheep, respectively. However, <i>M. haemolytica</i> isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among <i>P. multocida</i> and <i>M. haemolytica</i> isolates from lungs of cattle, sheep and goats.

scholarly journals Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 526-530 ◽  
Author(s):  
G. Ozbey ◽  
Ertas HB ◽  
A. Muz
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Random Primer ◽  
Serotype A ◽  
Dna Assay ◽  
Rapd Profile ◽  
Rapd Pattern ◽  
Serotype B ◽  
Dna Profiles ◽  
Ornithobacterium Rhinotracheale

Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the same serotype (A), different profiles were found but the strain of serotype (B) had an identical profile as strains of serotype A. This study suggests that more genotypes of ORT strains are present within the same serotype and thus that no relationship exists between the RAPD pattern of ORT and their serotype.

scholarly journals Application of Random Amplified Polymorphic DNA Markers for Identification of Marula Genotypes

HortScience ◽  
1999 ◽  
Vol 34 (7) ◽  
pp. 1256-1258 ◽  
Author(s):  
Feiga Gutman ◽  
Avinoam Nerd ◽  
Yosef Mizrahi ◽  
Dudy Bar-Zvi ◽  
Dina Raveh
Keyword(s):  
Dna Markers ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Randomly Amplified Polymorphic Dna ◽  
Distinct Band ◽  
Sclerocarya Birrea

Twenty-four genotypes of marula (Sclerocarya birrea subsp. caffra) were characterized using randomly amplified polymorphic DNA (RAPD) analysis. A distinct band pattern was obtained for each of the trees, using as few as four arbitrary 10-mer primers. Trees propagated vegetatively by grafting showed identical fingerprints. These results suggest that RAPD markers provide a useful system for documenting the identity of marula genotypes.

scholarly journals Identification of serotypes Pasteurella multocida and Mannheimia haemolytica from cattle and sheep in central Ethiopia

2019 ◽  
Author(s):  
Teshale Tolera ◽  
Abebe Wirtu ◽  
Bersissa Kumsa ◽  
Elsa Zerabruk ◽  
Yetnebersh Albene ◽  
...  
Keyword(s):  
Management System ◽  
Pasteurella Multocida ◽  
Bacterial Species ◽  
Mannheimia Haemolytica ◽  
Intensive Management ◽  
Pneumonic Pasteurellosis ◽  
Etiologic Agents ◽  
Nasal Swabs ◽  
Positive Results ◽  
Cattle And Sheep

Abstract Background: Pneumonic pasteurellosis is a multi-factorial respiratory disease of cattle and sheep caused by combination of etiologic agents; hence, reliable information is needed on the species or serotypes of bacterial agents for optimum control of the disease. This study was conducted with the objectives of identification of bacterial agents causing pneumonic pasteurellosis in cattle and sheep and identify serotypes of P. multocida involved. Methods: Bacteriological and molecular methods were used on 176 pneumonic lungs (93 cattle and 83 sheep) collected from abattoirs and 604 nasal swabs collected from 302 cattle and 302 sheep presented to Asella, Holota and Sheno veterinary clinics. Results: Twenty-five percent, 26.5% and 23.5% of nasal swabs collected from cattle and sheep from Asella, Holota and Sheno, respectively, were positive to one or more species of Pasteurella, Mannheimia and Bibersteinia. Mannheimia haemolytica was the predominant bacteria isolated at all sites. The isolation of these bacterial species was associated with age and pneumonic status of animals and management system. Young animals were more likely to yield positive results than adults (OR = 1.56; 95 % CI: 1.02, 2.38). Similarly, isolation of the three bacterial species was more frequent in animals with signs of pneumonia than those animals without signs of pneumonia (OR = 4.67; 95 % CI: 3.03, 7.19) and from animals under intensive management system than those animals kept under extensive management system (OR = 2.46; 95 % CI: 1.12, 5.39). Out of the total of 176 pneumonic lungs examined isolation was done from 48 (27.27 %) of them. Mannheimia haemolyitica was the predominant species isolated from pneumonic lungs. The identity of P. multocida and M. haemolytica isolated was further confirmed using PCR. Pasteurella multocida serotypes A1 and A3 and M. haemolytica serotype A1 were the predominant serotypes identified. Conclusion: This study revealed that M. haemolytica, P. multocida and B. trehalosi are commonly circulating in cattle and sheep originated from various parts of the country. It is also interesting that the serotypes of P. multocida and M. haemolytica identified in the present study are those that are already proven to cause pneumonia in ruminants.

scholarly journals Molecular characterization of Malassezia sympodialis and Malassezia furfur from cattle with and without otitis

2008 ◽  
Vol 60 (4) ◽  
pp. 779-785 ◽  
Author(s):  
E.R. Duarte ◽  
J.S. Hamdan
Keyword(s):  
Genetic Heterogeneity ◽  
Inflammatory Process ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Study ◽  
Malassezia Furfur ◽  
Geographic Origins ◽  
Minas Gerais State

A molecular study of Malassezia strains isolated from cattle with or without otitis was carried out by random amplified polymorphic DNA analysis (RAPD). DNA was extracted and purified from nine strains of Malassezia sympodialis and fourteen of Malassezia furfur. These microorganisms were collected from eight different bovine herds in Minas Gerais state, Brazil. The RAPD analysis and phenograms did not show the formation of genetically distinct groups among the strain isolated from cattle with or without otitis raised in the same herds. Genetic heterogeneity was observed among Malassezia strains from different geographic origins. These data suggest that genetically similar M. sympodialis and M. furfur strains found as members of the normal ear microbiota could become opportunistically active in the inflammatory process in cattle.

scholarly journals Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of Pasteurella multocida isolated from Korean Pigs

2012 ◽  
Vol 74 (5) ◽  
pp. 567-573 ◽  
Author(s):  
Ki-Eun LEE ◽  
Hye-Young JEOUNG ◽  
Ji-Youn LEE ◽  
Myoung-Heon LEE ◽  
Hwan-Won CHOI ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Phenotypic Characterization

scholarly journals First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

2021 ◽  
Vol 9 (6) ◽  
pp. 1322
Author(s):  
Tamara Alhamami ◽  
Piklu Roy Chowdhury ◽  
Nancy Gomes ◽  
Mandi Carr ◽  
Tania Veltman ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Antimicrobial Agents ◽  
Random Amplified Polymorphic Dna ◽  
Tetracycline Resistance ◽  
Multidrug Resistant ◽  
Mannheimia Haemolytica ◽  
Resistant Isolate ◽  
High Morbidity ◽  
Resistant Phenotype ◽  
South Wales

Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014–2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and blaROB-1 encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored.

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum

2019 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
O-Chul Kwon ◽  
Chang-Soo Lee ◽  
Young-Jin Park
Keyword(s):  
Ganoderma Lucidum ◽  
Gene Sequence ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characteristics ◽  
Pcr Analysis ◽  
Scar Markers ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Specific Fragment

In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped G. lucidum strains were analyzed and aligned, an SNP was found only in the antler-shaped G. lucidum strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by HinfI restriction enzyme. We further analyzed the polymorphism of various G. lucidum strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped G. lucidum strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped G. lucidum strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of G. lucidum.

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