scholarly journals Identification of serotypes Pasteurella multocida and Mannheimia haemolytica from cattle and sheep in central Ethiopia

2019 ◽  
Author(s):  
Teshale Tolera ◽  
Abebe Wirtu ◽  
Bersissa Kumsa ◽  
Elsa Zerabruk ◽  
Yetnebersh Albene ◽  
...  
Keyword(s):  
Management System ◽  
Pasteurella Multocida ◽  
Bacterial Species ◽  
Mannheimia Haemolytica ◽  
Intensive Management ◽  
Pneumonic Pasteurellosis ◽  
Etiologic Agents ◽  
Nasal Swabs ◽  
Positive Results ◽  
Cattle And Sheep

Abstract Background: Pneumonic pasteurellosis is a multi-factorial respiratory disease of cattle and sheep caused by combination of etiologic agents; hence, reliable information is needed on the species or serotypes of bacterial agents for optimum control of the disease. This study was conducted with the objectives of identification of bacterial agents causing pneumonic pasteurellosis in cattle and sheep and identify serotypes of P. multocida involved. Methods: Bacteriological and molecular methods were used on 176 pneumonic lungs (93 cattle and 83 sheep) collected from abattoirs and 604 nasal swabs collected from 302 cattle and 302 sheep presented to Asella, Holota and Sheno veterinary clinics. Results: Twenty-five percent, 26.5% and 23.5% of nasal swabs collected from cattle and sheep from Asella, Holota and Sheno, respectively, were positive to one or more species of Pasteurella, Mannheimia and Bibersteinia. Mannheimia haemolytica was the predominant bacteria isolated at all sites. The isolation of these bacterial species was associated with age and pneumonic status of animals and management system. Young animals were more likely to yield positive results than adults (OR = 1.56; 95 % CI: 1.02, 2.38). Similarly, isolation of the three bacterial species was more frequent in animals with signs of pneumonia than those animals without signs of pneumonia (OR = 4.67; 95 % CI: 3.03, 7.19) and from animals under intensive management system than those animals kept under extensive management system (OR = 2.46; 95 % CI: 1.12, 5.39). Out of the total of 176 pneumonic lungs examined isolation was done from 48 (27.27 %) of them. Mannheimia haemolyitica was the predominant species isolated from pneumonic lungs. The identity of P. multocida and M. haemolytica isolated was further confirmed using PCR. Pasteurella multocida serotypes A1 and A3 and M. haemolytica serotype A1 were the predominant serotypes identified. Conclusion: This study revealed that M. haemolytica, P. multocida and B. trehalosi are commonly circulating in cattle and sheep originated from various parts of the country. It is also interesting that the serotypes of P. multocida and M. haemolytica identified in the present study are those that are already proven to cause pneumonia in ruminants.

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

2012 ◽  
Vol 49 (No. 3) ◽  
pp. 65-69 ◽  
Author(s):  
G. Ozbey ◽  
A. Kilic ◽  
H. B Ertas ◽  
A. Muz
Keyword(s):  
Genetic Heterogeneity ◽  
Pasteurella Multocida ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mannheimia Haemolytica ◽  
Random Primer ◽  
Sheep And Goats ◽  
Distinct Band ◽  
Cattle And Sheep

n this study, 30, 15 and 1 strains of <i>Pasteurella multocida</i> and 9, 8 and 6 strains of <i>Mannheimia haemolytica</i> from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of <i>P. multocida</i> isolates from cattle and sheep, respectively. However, <i>M. haemolytica</i> isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among <i>P. multocida</i> and <i>M. haemolytica</i> isolates from lungs of cattle, sheep and goats.

scholarly journals Research on reproductive performance of mares in Serbia using bacteriological examination

2011 ◽  
Vol 27 (3) ◽  
pp. 883-892
Author(s):  
M.I. Urosevic ◽  
D. Stojanovic ◽  
B. Lako ◽  
I. Jajic ◽  
Z. Milicic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reproductive Performance ◽  
Staphylococcus Epidermidis ◽  
Pasteurella Multocida ◽  
Bacterial Species ◽  
The Other ◽  
Streptococcus Zooepidemicus ◽  
Bacteriological Examination ◽  
Reproductive Disorders ◽  
Bacillus Spp

The research was conducted on 19 stud farms in Serbia, on 80 mares used for breeding, with and without reproductive disorders. During the two years period (from 2009 to 2010) double guarded uterine swabs from 80 mares, aged between 3 and 22 years were collected. Mares belonged to the different breeds: Thoroughbred, Standardbred, Lipizzaner and mixed breeds. It was determined, that bacterial infection of genital organs was found in 24 mares in the examined population, and the bacterial species Streptococcus zooepidemicus was diagnosed in the 11 samples from cervical swabs. In the 5 samples, Escherichia coli was isolated, while Staphylococcus epidermidis and Pasteurella multocida were present in the 2 samples each, while the other causes and simultaneous isolation of two bacterial species are much less present. These species are: Bacillus spp. plus Escherichia coli; Streptococcus zooepidemicus plus Klebsiella pneumoniae and Escherichia coli plus Streptococcus zooepidemicus. In one swab we determinated Arcanobacter pyogenes. In this examination, according to available data after natural mating, we found conception level of 43,10%, which is similar with previous reports in our country.

scholarly journals Crossbred Local Chickens from East Nusa Tenggara Province Indonesia Under Semi Intensive Management System

2021 ◽  
Vol 748 (1) ◽  
pp. 012003
Author(s):  
N.G.A Mulyantini S.S ◽  
Ulrikus R Lole
Keyword(s):  
Weight Gain ◽  
Artificial Insemination ◽  
Management System ◽  
Egg Production ◽  
Abdominal Fat ◽  
Production Performance ◽  
The Other ◽  
Intensive Management ◽  
Commercial Grower ◽  
Local Chickens

Abstract The objective of this study was to compare the performance of four-breed combination under semiintensive management system. The experiment used 160 females and 40 males chickens as parents. Artificial insemination was performed to produce the experimental chicken of the four breed combinations (1. Sabu x Sabu, 2. Sabu x Semau, 3. Sabu x KUB, 4. Semau x KUB). The chickens were fed with chick starter crumb sad libitum from day old to 4 weeks of age. From 4-8 weeks of age, chickens were allowed to scavenge during the day, and also given commercial grower pellets + rice bran + corn (5:4:1). Combination of Sabu x KUBfrom 4-8 weeks of age were significantly heavier (P < 0.05) than the other combination breeds. Feed intake in the Sabu x KUB group was the lowest, and their weight gain was the highest among the others group(P < 0.05). The combination Sabu x KUB also had the highest egg production, and hatchability. However, the percentage of abdominal fat of Sabu x KUB were higher than others groups. In conclusion, combination Sabu x KUB had the best growth performance and egg production performance, but not for the percentage of abdominal fat.

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