scholarly journals DNA marker of Tilletia controversa Kühn, a causal agent of wheat dwarf bunt

2012 ◽  
Vol 42 (Special Issue) ◽  
pp. 13-13
Author(s):  
W.-Q. Chen ◽  
T.-G. Liu ◽  
J.-H. Liu ◽  
S.-Ch. Xu
Keyword(s):  
Full Text ◽  
Dna Marker ◽  
Causal Agent ◽  
Tilletia Controversa ◽  
Dwarf Bunt

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scholarly journals Methyljasmonate and salicylic acid contribute to the control of Tilletia controversa Kühn, causal agent of wheat dwarf bunt

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ghulam Muhae-Ud-Din ◽  
Delai Chen ◽  
Taiguo Liu ◽  
Wanquan Chen ◽  
Li Gao
Keyword(s):  
Fungal Pathogen ◽  
Causal Agent ◽  
Disease Symptoms ◽  
Defence Genes ◽  
The World ◽  
Tilletia Controversa ◽  
High Level ◽  
And Control ◽  
Dwarf Bunt

Abstract Tilletia controversa Kühn (TCK) is the causal agent of dwarf bunt of wheat, a destructive disease in wheat-growing regions of the world. The role of Meja, SA and Meja + SA were characterized for their control of TCK into roots, coleoptiles and anthers. The response of the defence genes PR-10a, Catalase, COI1-1, COII-2 and HRin1 was upregulated by Meja, SA and Meja + SA treatments, but Meja induced high level of expression compared to SA and Meja + SA at 1, 2, and 3 weeks in roots and coleoptiles, respectively. The severity of TCK effects in roots was greater at 1 week, but it decreased at 2 weeks in all treatments. We also investigated TCK hyphae proliferation into coleoptiles at 3 weeks and into anthers to determine whether hyphae move from the roots to the upper parts of the plants. The results showed that no hyphae were present in the coleoptiles and anthers of Meja-, SA- and Meja + SA-treated plants, while the hyphae were located on epidermal and sub-epidermal cells of anthers. In addition, the severity of hyphae increased with the passage of time as anthers matured. Bunted seeds were observed in the non-treated inoculated plants, while no disease symptoms were observed in the resistance of inducer treatments and control plants. Plant height was reduced after TCK infection compared to that of the treated inoculated and non-inoculated treatments. Together, these results suggested that Meja and SA display a distinct role in activation of defence genes in the roots and coleoptiles and that they eliminate the fungal pathogen movement to upper parts of the plants with the passage of time as the anthers mature.

scholarly journals Characterization of the wheat cultivars against Tilletia controversa Kühn, causal agent of wheat dwarf bunt

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ghulam Muhae-Ud-Din ◽  
Delai Chen ◽  
Taiguo Liu ◽  
Wanquan Chen ◽  
Li Gao
Keyword(s):  
Causal Agent ◽  
Wheat Cultivars ◽  
Tilletia Controversa ◽  
Dwarf Bunt

Tilletia controversa. [Descriptions of Fungi and Bacteria].

1983 ◽  
Author(s):  
J. M. Waller
Keyword(s):  
Winter Wheat ◽  
Geographical Distribution ◽  
Early Stage ◽  
Winter Barley ◽  
Tilletia Controversa ◽  
Dwarf Bunt

Abstract A description is provided for Tilletia controversa. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Aegilops, Agropyron, Alopecurus, Arrhenatherum, Beckmannia, Bromus, Dactylis, Elymus, Festuca, Holcus, Hordeum, Koehleria, Lolium, Poa, Secale, Triticum, Trisetum. DISEASE: Causes dwarf bunt of winter wheat, and occurs sporadically on many grasses. Occasionally infects winter barley. Infected plants develop chlorotic flecks at an early stage, are markedly stunted, and produce rather fat, persistently green ears with protuberant spikes caused by the bunt balls which fill the grain. As with T. caries (CMI Descriptions No. 719) and T. foetida (CMI Descriptions No. 720) the contents of the grain are converted to a mass of teliospores which constitute the bunt ball. GEOGRAPHICAL DISTRIBUTION: Europe (except Spain and UK); N. Africa, W. Asia, N. America, Argentina and Uruguay (CMI Map 297, ed. 2, 1968). TRANSMISSION: Teliospores are released when the grain is harvested and contaminate soil and seed. Soil-borne spores are the major source of inoculum for infecting crops which occurs between December and April in NW USA (43, 1295). Teliospores in bunt balls can remain viable in the soil for several years.

scholarly journals Laboratory diagnostics of common bunt and dwarf bunt

2012 ◽  
Vol 42 (Special Issue) ◽  
pp. 75-77
Author(s):  
M. Kochanová ◽  
E. Prokinová ◽  
P. Ryšánek
Keyword(s):  
Full Text ◽  
Laboratory Diagnostics ◽  
Common Bunt ◽  
Dwarf Bunt

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A linear plasmid in Tilletia controversa, a fungal pathogen of wheat

1988 ◽  
Vol 66 (6) ◽  
pp. 1098-1100 ◽  
Author(s):  
W. K. Kim ◽  
S. A. McNabb ◽  
G. R. Klassen
Keyword(s):  
Fungal Pathogen ◽  
Physical Map ◽  
Restriction Enzymes ◽  
Ribosomal Genes ◽  
Puccinia Graminis ◽  
Ustilago Hordei ◽  
Tilletia Controversa ◽  
Total Dna ◽  
Exonuclease Digestion ◽  
Dwarf Bunt

A linear 7.4-kilobase plasmid designated pTCT was isolated from mycelium of Tilletia controversa Kühn, the causal agent of dwarf bunt of wheat. Linearity was determined by construction of a physical map using eight restriction enzymes and by both 5′ and 3′ exonuclease digestion. pTCT is not homologous to ribosomal genes, nor is it homologous to any sequences in the total DNA of sporidia of Ustilago hordei (isolates 965 and 966) or urediosporelings of Puccinia graminis f.sp. tritici (race C36(48)).

scholarly journals Metabolomics Analysis of Tilletia Controversa Kühn Infected and Non-infected Grains of Wheat

2020 ◽  
Author(s):  
Zhaoyu Ren ◽  
Ghulam Muhae-Ud-Din ◽  
Jianjian Liu ◽  
Taiguo Liu ◽  
Wanquan Chen ◽  
...  
Keyword(s):  
Tissue Level ◽  
Isoquinoline Alkaloid ◽  
Sphingolipid Metabolism ◽  
Liquid Chromatography Mass Spectrometry ◽  
Related Substances ◽  
Tilletia Controversa ◽  
Tyrosine Metabolism ◽  
Phenylalanine Metabolism ◽  
Dwarf Bunt ◽  
Metabolomic Approach

Abstract Dwarf bunt caused by the pathogen Tilletia controversa Kühn is one of the most serious quarantine disease of winter wheat. Metabolomics studies provide detailed information about biochemical changes at the cell and tissue level of the plants. In the present study, liquid chromatography/mass spectrometry (LC/MS) metabolomic approach was used to investigate the changes in the grains metabolomics of T. controversa infected and non-infected samples. PCA analysis suggested that T. controversa infected and non-infected samples scattered separately during the interaction. LC/MS analysis showed that 62 different metabolites were recorded in the grains, among them total of 34 metabolites were up-regulated and 28 metabolite were down-regulated. The prostaglandins (PGs) and 9-hydroxyoctadecaenoic acids (9-HODEs) are fungal toxin related substances and their expression significantly increased in T. controversa infected grains. Additionally, the concentration of cucurbic acid and octadecatrienoic acid were changed significantly after pathogen infection, which has great role in plant defense. The eight different metabolic pathways activated during the T. controversa and wheat plants interactions including phenylalanine metabolism, isoquinoline alkaloid biosynthesis, starch and sucrose metabolism, tyrosine metabolism, sphingolipid metabolism, arginine and proline metabolism, alanine, aspartate, glutamate metabolism, and tryptophan metabolism.

scholarly journals Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhaoyu Ren ◽  
Jianjian Liu ◽  
Ghulam Muhae Ud Din ◽  
Han Zhang ◽  
Zhenzhen Du ◽  
...  
Keyword(s):  
Differential Expression Analysis ◽  
Enrichment Analysis ◽  
Mitogen Activated Protein Kinase ◽  
Transcriptome Profile ◽  
Resistance Level ◽  
New Methods ◽  
Tilletia Controversa ◽  
Mitogen Activated Protein ◽  
Mock Infection ◽  
Dwarf Bunt

AbstractWheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes.

Low-temperature storage of monosporidial cultures of the wheat bunt fungi

1995 ◽  
Vol 73 (5) ◽  
pp. 758-760 ◽  
Author(s):  
Patricia Loomis ◽  
Hei Leung
Keyword(s):  
Somatic Mutations ◽  
Interspecific Crosses ◽  
Meiotic Segregation ◽  
Serial Transfer ◽  
Tilletia Tritici ◽  
Storage Method ◽  
Low Temperature Storage ◽  
Tilletia Controversa ◽  
Dwarf Bunt ◽  
Temperature Storage

Collections of Tilletia species are commonly maintained in the form of sori harvested from smutted heads. This has been a convenient and effective storage method because teliospores enclosed in a sorus remain viable for over 20 years. However, mycelial cultures derived from germinated teliospores are genetically mixed because of meiotic segregation among sporidia. For the purposes of genetic analysis and establishing strain identity, it is necessary to maintain haploid sporidial cultures that can be readily retrieved for manipulations. We found that sporidial cultures of Tilletia tritici and Tilletia controversa can be preserved for at least 1 year by suspending a mixture of mycelia and sporidia in 15% glycerol and storing at −70 °C. Approximately 80% of the stored cultures were viable after 12 months in storage. Five out of nine monosporidial cultures of T. tritici and T. controversa tested retained their sexual fertility in interspecific crosses. The glycerol storage method has advantages over serial transfer in the maintenance of monosporidial cultures by reducing the accumulation of somatic mutations; however, the technique should be used cautiously until greater infectivity of stored cultures is demonstrated. Key words: common and dwarf bunt fungi, Tilletia controversa, Tilletia tritici.

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