scholarly journals Unusual behavior of natural polyphosphates during IMAC

2013 ◽  
Vol 19 (No. 5) ◽  
pp. 161-165
Author(s):  
M. Kmínková ◽  
A. Prošková ◽  
J. Kučera
Keyword(s):  
Phytic Acid ◽  
Iron Content ◽  
Metal Ion ◽  
Ferric Ion ◽  
Ferric Ions ◽  
Unusual Behavior ◽  
Strong Bond ◽  
Hydrolysis Of ◽  
Chelating Groups ◽  
Metal Ion Affinity Chromatography

The atypical course of IDA-ferric (Imino-Di-Acetyl-agarose saturated with ferric ions) of two polyphosphorylated compounds – phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with IDA (Imino-Di-Acetate) itself. Therefore attention is to be paid to all IMAC (Immobilized-Metal-Ion-Affinity-Chromatography) of natural compounds containing strong chelating groups or several chelating groups in vicinity.

INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

1961 ◽  
Vol 38 (4) ◽  
pp. 545-562 ◽  
Author(s):  
L. Kecskés ◽  
F. Mutschler ◽  
I. Glós ◽  
E. Thán ◽  
I. Farkas ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Granulosa Cell ◽  
Iron Content ◽  
List Type ◽  
Granulosa Cell Tumour ◽  
Hydrolysis Of ◽  
Phenol Fraction ◽  
Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

Adsorption of endotoxins on Ca2+ iminodiacetic acid by metal ion affinity chromatography

2013 ◽  
Vol 30 (11) ◽  
pp. 1194-1202
Author(s):  
LOPES André Moreni ◽  
ROMEU Jorge Sánchez ◽  
MEIRELES Rolando Páez ◽  
PERERA Gabriel Marquez ◽  
MORALES Rolando Perdomo ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Iminodiacetic Acid ◽  
Metal Ion Affinity Chromatography

Metal Ion Catalysis in the Hydrolysis of Potassium Ethyl Oxalate

Nature ◽  
1964 ◽  
Vol 204 (4964) ◽  
pp. 1189-1190
Author(s):  
ROBERT W. HAY ◽  
NEIL J. WALKER
Keyword(s):  
Metal Ion ◽  
Metal Ion Catalysis ◽  
Hydrolysis Of

scholarly journals Blue flower coloration of Corydalis ambigua requires ferric ion and kaempferol glycoside

2021 ◽  
Vol 85 (1) ◽  
pp. 61-68
Author(s):  
Kumi Yoshida ◽  
Takeya Oniduka ◽  
Kin-ichi Oyama ◽  
Tadao Kondo
Keyword(s):  
Emission Spectroscopy ◽  
Atomic Emission ◽  
Early Spring ◽  
Blue Flower ◽  
Ferric Ion ◽  
Ferric Ions ◽  
Kaempferol Glycoside ◽  
Cotton Effects ◽  
Blue Coloration ◽  
Exciton Type

Abstract Corydalis ambigua (Japanese name, Ezoengosaku) flowers bloom with blue to purplish petals in early spring in Hokkaido prefecture. In this study, a mechanism for blue petal coloration by ferric ions and keampferol glycoside was elucidated. Blue petals and cell sap exhibited similar visible (Vis) spectra, with λmax at approximately 600 nm and circular dichroism (CD) with positive exciton-type Cotton effects in the Vis region. Analysis of the organic components of the petals confirmed cyanidin 3-O-sambubioside and kaempferol 3-O-sambubioside as the major flavonoids. Mg, Al, and Fe were detected in petals using atomic emission spectroscopy. Color, Vis absorption, and CD consistent with those of blue petals were reproduced by mixing cyanidin 3-O-sambubioside, kaempferol 3-O-sambubioside, and Fe3+ in a buffered aqueous solution at pH 6.5. Both Fe3+ and flavonol were essential for blue coloration.

Sequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography

2013 ◽  
Vol 86 (1) ◽  
pp. 685-693 ◽  
Author(s):  
Chia-Feng Tsai ◽  
Chuan-Chih Hsu ◽  
Jo-Nan Hung ◽  
Yi-Ting Wang ◽  
Wai-Kok Choong ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Metal Ion Affinity Chromatography

Metal ion effects on the acid hydrolysis of pyrophosphate

1964 ◽  
Vol 26 (8) ◽  
pp. 1415-1420 ◽  
Author(s):  
J.M. Rainey ◽  
M.M. Jones ◽  
W.L. Lockhart
Keyword(s):  
Acid Hydrolysis ◽  
Metal Ion ◽  
Ion Effects ◽  
Metal Ion Effects ◽  
Hydrolysis Of

MetLigDB: a web-based database for the identification of chemical groups to design metalloprotein inhibitors

2011 ◽  
Vol 44 (4) ◽  
pp. 878-881 ◽  
Author(s):  
Hwanho Choi ◽  
Hongsuk Kang ◽  
Hwangseo Park
Keyword(s):  
Drug Discovery ◽  
Metal Ions ◽  
Metal Ion ◽  
Central Metal ◽  
Amino Acid Residues ◽  
Web Based ◽  
Additional Information ◽  
Chemical Groups ◽  
Insight Into ◽  
Chelating Groups

MetLigDB (http://silver.sejong.ac.kr/MetLigDB) is a publicly accessible web-based database through which the interactions between a variety of chelating groups and various central metal ions in the active site of metalloproteins can be explored in detail. Additional information can also be retrieved, including protein and inhibitor names, the amino acid residues coordinated to the central metal ion, and the binding affinity of the inhibitor for the target metalloprotein. Although many metalloproteins have been considered promising targets for drug discovery, it is difficult to discover new inhibitors because of the difficulty in designing a suitable chelating moiety to impair the catalytic activity of the central metal ion. Because both common and specific chelating groups can be identified for varying metal ions and the associated coordination environments, MetLigDB is expected to give users insight into designing new inhibitors of metalloproteins for drug discovery.

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