scholarly journals The effect of micro-sized titanium dioxide on WM-266-4 metastatic melanoma cell line

2019 ◽  
Vol 19 (1) ◽  
pp. 60-66
Author(s):  
Tanja Prunk Zdravković ◽  
Bogdan Zdravković ◽  
Mojca Lunder ◽  
Polonca Ferk
Keyword(s):  
Titanium Dioxide ◽  
Mrna Expression ◽  
Metastatic Melanoma ◽  
Cell Line ◽  
Metabolic Activity ◽  
Melanoma Cell Line ◽  
Melanoma Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Metastatic Melanoma Cell

Titanium dioxide (TiO2) is widely used as an inorganic UV-filter in cosmetic products; however, it has been classified as possibly carcinogenic to humans. While numerous studies demonstrated cytotoxic and genotoxic effects of nano-sized TiO2 in different cell lines, including human skin cells, studies investigating the effects of micro-TiO2 on human keratinocytes and melanocytes, in healthy and cancer cells, are scarce. Adenosine triphosphate (ATP) binding cassette subfamily B member 5 (ABCB5) is a plasma membrane protein known for its role in the tumorigenicity, progression, and recurrence of melanoma. Here, we investigated the effect of micro-TiO2 (average particle size ≤5 µm) on the metabolic activity, cytotoxicity and ABCB5 mRNA expression in metastatic melanoma cells. Metastatic melanoma cell line WM-266-4 was treated with different concentrations of micro-TiO2 for different incubation times to obtain dose- and time-dependent responses. Untreated WM-266-4 cells, cultured under the same conditions, were used as control. The cell metabolic activity was determined by MTT assay. Cytotoxicity of micro-TiO2 was analyzed by lactate dehydrogenase (LDH) cytotoxicity assay. The ABCB5 mRNA expression in melanoma cells was analyzed using quantitative reverse transcription polymerase chain reaction (RT-qPCR). After 120 hours of exposure to micro-TiO2 the metabolic activity of melanoma cells decreased, especially at the two highest micro-TiO2 concentrations. Comparably, the cytotoxicity of micro-TiO2 on melanoma cells increased after 48 and 120 hours of exposure, in a time-dependent manner. The ABCB5 mRNA expression in micro-TiO2-treated melanoma cells also decreased significantly after 24 and 48 hours, in a time-dependent manner. Overall, our results suggest inhibitory effects of micro-TiO2 on the metabolic activity and ABCB5 mRNA expression in metastatic melanoma cells, indicating its potential use as an anticancer agent.

Study on Mechanisms of Telomerase Regulation in Arsenic Trioxide Inducing Apoptosis in Myelodysplasia Cell Line.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4706-4706
Author(s):  
Hongyan Tong ◽  
Jie Jin ◽  
Weilai Xu ◽  
Wenbin Qian ◽  
Maofang Lin
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Telomerase Activity ◽  
Time Dependent ◽  
Dependent Manner ◽  
Htert Gene ◽  
Binding Factor ◽  
Telomerase Regulation ◽  
Dose Dependent ◽  
Ttaggg Repeat

Abstract The telomerase activity can be down regulated by arsenic trioxide (As2O3), which is regarded as an apoptotic induction agent, is confirmed in many kinds of tumor cells. To investigate the mechanisms of telomerase regulation and to explore the correlation of As2O3 inducing apoptosis and telomerase regulation in MUTZ-1 cells, which are established as a high-risk myelodysplasia Cell line that derived from a MDS patient (FAB subtype refractory anemia with excess of blasts), a quantitative assessment of the telomerase activity by TRAP-ELISA and detection of the expression levels of hTERT, TRF1 (TTAGGG repeat binding factor 1), TRF2 (TTAGGG repeat binding factor 2), bcl-2, bax mRNA were performed, together with the assessment of the apoptosis by means of translocation of phosphatidylserine (PS) through flow cytometry assay. The results indicated that a typical apoptotic cell group distribution of DNA content was represented in the MUTZ-1 cells after being exposed to As2O3 at the range of concentration from 1μmol/L to 8μmol/L in a dose-dependent manner (r=0.736, P<0.001) and time-dependent manner (r=0.674, p<0.05), and the telomerase activity was down-regulated in a time-dependent manner (r=−0.976,P=0.024), and the expression level of hTERT mRNA in MUTZ-1 cells was represented in a dose-dependent manner (r=−0.892,P=0.042) and time-dependent manner (r=−1.000,P=0.04), after the cells were treated by As2O3 at the dosage as above. It was showed that a significant correlation between the decreased telomerase activity and the increased percentage of apoptotic cells in the treated cells (r=0.938,P=0.018), and there was a strong relationship between the telomerase activity and the mRNA expression of hTERT gene (r=0.783,P=0.022). However, As2O3 has no obvious effect on the expression level of TRF1 mRNA and TRF2 mRNA, which were regarded as two telomere-binding proteins. Further findings indicated that the inhibition of telomerase activity in MUTZ-1 cells was accompanied with down-regulated mRNA expression of bcl-2 gene (densitometry readings: 0.255±0.017 vs 0.466±0.069, P<0.05) and decreased ration of bcl-2/bax (densitometry reading ratios: 0.890±0.083 vs 0.546±0.014, P<0.05) at the dosage of 4μmol/L for 24 hours. These observations suggest that the apoptosis induced by As2O3 on MUTZ- 1 cells might be mediated through the inhibition of telomerase activity regulated by expression of hTERT gene, which implies that may be one of the mechanisms of As2O3 inducing apoptosis in MUTZ-1 cells.

Establishment of a novel Chinese metastatic melanoma cell line showing the new cytogenetic and biological properties

2015 ◽  
Vol 39 (4) ◽  
pp. 508-514
Author(s):  
Xiuli Yi ◽  
Guannan Zhu ◽  
Yan Li ◽  
Ying Liu ◽  
Qi Luan ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Biological Properties ◽  
Metastatic Melanoma Cell

In-vitro study of the influence of octocrylene on a selected metastatic melanoma cell line

2019 ◽  
Vol 154 (2) ◽  
Author(s):  
Tanja Prunk Zdravković ◽  
Bogdan Zdravković ◽  
Marko Zdravković ◽  
Barbara Dariš ◽  
Mojca Lunder ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
In Vitro Study ◽  
Vitro Study ◽  
Metastatic Melanoma Cell

H Ferritin Gene Silencing in a Human Metastatic Melanoma Cell Line: A Proteomic Analysis

2011 ◽  
Vol 10 (12) ◽  
pp. 5444-5453 ◽  
Author(s):  
Maddalena Di Sanzo ◽  
Marco Gaspari ◽  
Roberta Misaggi ◽  
Francesco Romeo ◽  
Lucia Falbo ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Metastatic Melanoma ◽  
Cell Line ◽  
Melanoma Cell ◽  
Proteomic Analysis ◽  
Melanoma Cell Line ◽  
Metastatic Melanoma Cell ◽  
Ferritin Gene

scholarly journals Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Parvathi Haridas ◽  
Jacqui A. McGovern ◽  
Abhishek S. Kashyap ◽  
D. L. Sean McElwain ◽  
Matthew J. Simpson
Keyword(s):  
Metastatic Melanoma ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Metastatic Melanoma Cell

Establishment and characterization of a primary and a metastatic melanoma cell line from Grey horses

2013 ◽  
Vol 50 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Monika H. Seltenhammer ◽  
Elisabeth Sundström ◽  
Claudia Meisslitzer-Ruppitsch ◽  
Petra Cejka ◽  
Jedrzej Kosiuk ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Metastatic Melanoma Cell

scholarly journals Antiproliferative Activity of Selected Triterpene Acids from Rosemary on Metastatic Melanoma Cell Line WM-266-4

2021 ◽  
Vol 9 (B) ◽  
pp. 515-521
Author(s):  
Suzana Isaković-Vidović ◽  
Barbara Dariš ◽  
Željko Knez ◽  
Kristina Vidović ◽  
Dejan Oprić ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Cell Line ◽  
Incubation Period ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Metastatic Melanoma Cell ◽  
Triterpene Acids ◽  
Wide Range ◽  
Triterpenic Acids ◽  
Proliferation Activity

BACKGROUND: Natural products and their derivatives, particularly secondary metabolites, have been recognized for many years as an important source of therapeutic agents. In this context, pentacyclic triterpene acids including betulinic acid (BA), oleanolic acid (OA), and ursolic acid (UA) are highly valuable triterpenic acids because of their wide range of biological activities. AIM: Therefore, the aim of our study was to investigate any potential effect of BA, UA, and OA on human melanoma WM-266-4 cells’ proliferation activity. METHODS: BA, UA, and OA have been prepared in dimethyl sulfoxide in concentration range from 0.002 to 200 μM separately or in selected combination (UA+OA ratio 1:1 or 3.5:1), while cells in cell culture medium served as controls. The rapid colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was used to measure proliferation activity of the metastatic melanoma cell line WM-266-4 after being exposed to selected concentrations of BA, UA, OA, or UA+OA and during different time periods. Student’s t-test was used for single statistical comparisons. Data were analyzed using IBM SPSS 25.0 (IBM Corp., Armonk, NY). To account for multiple comparisons bias, p < 0.001 was considered statistically significant. RESULTS: Our results showed decreased cell proliferation activity after 4 h of incubation of WM-266-4 cells with BA, UA, OA, and UA+OA. The highest inhibitory effect was noted when cells were incubated with selected triterpenic acids and both combinations of UA+OA during the incubation period of 48 h. When compared to control cells, concentration of 2 μM was the lowest concentration of BA that showed a significant decrease of the cells’ proliferation activity regardless the incubation period (4 h, 24 h, and 48 h) (p < 0.001). CONCLUSION: Our encouraging results could be a good starting point for further studies on possible use of BA, UA, and OA in prevention and treatment of metastatic melanoma.

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