The emerging of bacteria/cell culturing in biological/biomedical research and industry is in demand for low cost, fast, non-invasive, and reliable alternative/approach for evaluation and measurement. Microfluidic approach is one of the promising alternatives for replacing the expensive commercial cuvvete for bacteria/cell culture and suspension for optical measurement. This study demonstrates the integration of absorbance measurement with microfluidic platform forEscherichia coli(E. coli) bacteria suspension analysis. TheE. coliwas cultured and prepared for suspension medium which then transferred inside the PDMS-glass based microfluidic. Then, the absorbance measurement is carried out using UV-Visible spectrophotometer. We demonstrate this method by measuring absorption of light transmitted through microfluidic chambers within the visible light range (350nm - 750nm). From the result, it had indicates that the graph pattern and growth behavior ofE. colisuspension in microfluidic platform are reliable and comparable to commercial cuvvete reading. This finding
A low-cost absorbance measurement device using microcontroller
