Static Incubation of Pancreatic Islets v1

2021 ◽  
Author(s):  
Islet and Pancreas Analysis Core
Keyword(s):  
Pancreatic Islets ◽  
Human Tissue ◽  
Islet Isolation ◽  
Diabetes Center

This SOP defines the methods used by the Vanderbilt Diabetes Center Islet and Pancreas Analysis (IPA) Core for static incubation of pancreatic islets isolated from mouse or human tissue. See also our islet isolation protocol.

Mouse Pancreatic Islet Isolation v1

2021 ◽  
Author(s):  
Islet and Pancreas Analysis Core
Keyword(s):  
Transgenic Mice ◽  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Islet Isolation ◽  
Wild Type ◽  
Pancreatic Islet Isolation ◽  
Diabetes Center ◽  
Mouse Pancreatic Islet ◽  
Isolation Of Pancreatic Islets

This SOP defines the methods used by the Vanderbilt Diabetes Center Islet and Pancreas Analysis (IPA) Core for isolation of pancreatic islets from wild type and transgenic mice.

Pancreatic Islet RNA Extraction v1

2021 ◽  
Author(s):  
Islet and Pancreas Analysis Core
Keyword(s):  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Human Tissue ◽  
Rna Extraction ◽  
Diabetes Center

This SOP defines the methods used by the Vanderbilt Diabetes Center Islet and Pancreas Analysis (IPA) Core for RNA extraction of pancreatic islets isolated from mouse or human tissue.

scholarly journals A Comparison of Pancreatic Islet Purification using Iodixanol with University of Wisconsin Solution and with Na-Lactobionate and Histidine Solution

Cell Medicine ◽  
2018 ◽  
Vol 10 ◽  
pp. 215517901877507 ◽  
Author(s):  
Yoshiki Nakashima ◽  
Chika Miyagi-Shiohira ◽  
Nana Ebi ◽  
Eri Hamada ◽  
Yoshihito Tamaki ◽  
...  
Keyword(s):  
Survival Rate ◽  
Pancreatic Islets ◽  
Stimulation Index ◽  
Islet Isolation ◽  
University Of Wisconsin Solution ◽  
Porcine Islet ◽  
University Of Wisconsin ◽  
Purification Methods ◽  
Isolation Performance ◽  
Wisconsin Solution

Purification of pancreatic islets is an important step in islet isolation for islet transplantation. In this study, to investigate how a solution composed mainly of Na-lactobionate and histidine (HL) influences the purification of islets, iodixanol was added to a purified solution for porcine islet isolation. A solution (IU) made by adding iodixanol to University of Wisconsin solution and a solution (IHL) made by adding iodixanol to HL solution were used to evaluate the islet isolation performance. We noted no significant differences between the two purification methods with regard to the islet yield, survival rate or purity, score, or stimulation index. These results show that IHL solution is as useful as IU solution for islet purification.

scholarly journals Pancreatic islet isolation by mechanical-enzymatic separation, stationary collagenase digestion and dextran discontinuous density gradient purification: experimental study in dogs

2002 ◽  
Vol 17 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Jaques Waisberg ◽  
Heloísa Prado Soares ◽  
Luís Gustavo Altieri ◽  
Osíris Ramaciotti ◽  
Manlio Basilio Speranzini
Keyword(s):  
Experimental Study ◽  
Body Weight ◽  
Pancreatic Islets ◽  
Density Gradient ◽  
Gradient Methods ◽  
Islet Isolation ◽  
Single Donor ◽  
Collagenase Digestion ◽  
Pancreatic Islet Isolation ◽  
Density Gradient Purification

The prospects for allotransplantation of pancreatic islets in man depend on the development of methods that provide sufficient quantities of pancreatic islets from a single donor, which are capable, when transplanted, of achieve the normalization of carbohydrate metabolism. Objective: Evaluate the efficacy of the isolation of Langerhans islets from dogs, by means of mechanical-enzymatic separation technique with stationary digestion using collagenase, and purification with a discontinuous dextran density gradient. Methods: The counting of islet numbers and evaluation of their sizes was accomplished by staining with diphenylthiocarbazone and using stereoscopic microscopes equipped with eyepiece reticule for the measurement of average diameters of stained islets. Results: The results disclosed that the average number of islets isolated was 81032.20 ± 24736.79 and the average number of islets isolated per kg of body weight was 6938.70 ± 1392.43. The average number of islets isolated per kg of body weight showed significant correlation with body weight and weight of the pancreas resected. Conclusion: The number of islets isolated, of a single donor, by mechanical-enzymatic separation, stationary collagenase digestion and discontinuous dextran density gradient purification can be sufficient to success of pancreatic islets transplant in dogs.

scholarly journals ANALYSIS OF ADENOVIRAL MEDIATED GENE TRANSFER TO PANCREATIC ISLETS BY VASCULAR PERFUSION OF THE INTACT PANCREAS PRIOR TO ISLET ISOLATION

1998 ◽  
Vol 66 (8) ◽  
pp. S41
Author(s):  
Shaoping Deng ◽  
Taras Kucher ◽  
Chengyang Liu ◽  
Haiying Chen ◽  
Kenneth L. Brayman
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islets ◽  
Islet Isolation ◽  
Vascular Perfusion

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

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