scholarly journals Multi-Patch Clamp Recording v1 (protocols.io.bcteiwje)

protocols.io ◽  
2020 ◽  
Author(s):  
Allen Institute
Keyword(s):  
Patch Clamp ◽  
Patch Clamp Recording

Measurement of spontaneous neuronal membrane activity by time lapse confocal microscopy

1995 ◽  
Vol 53 ◽  
pp. 972-973
Author(s):  
R H. Selinfreund ◽  
A. H. Cornell-Bell
Keyword(s):  
Membrane Potential ◽  
Confocal Microscopy ◽  
Patch Clamp ◽  
Electrical Activity ◽  
Time Lapse ◽  
Neuronal Firing ◽  
Neuronal Membrane ◽  
Pituitary Cells ◽  
Patch Clamp Recording ◽  
Spontaneous Electrical Activity

Cellular electrophysiological properties are normally monitored by standard patch clamp techniques . The combination of membrane potential dyes with time-lapse laser confocal microscopy provides a more direct, least destructive rapid method for monitoring changes in neuronal electrical activity. Using membrane potential dyes we found that spontaneous action potential firing can be detected using time-lapse confocal microscopy. Initially, patch clamp recording techniques were used to verify spontaneous electrical activity in GH4\C1 pituitary cells. It was found that serum depleted cells had reduced spontaneous electrical activity. Brief exposure to the serum derived growth factor, IGF-1, reconstituted electrical activity. We have examined the possibility of developing a rapid fluorescent assay to measure neuronal activity using membrane potential dyes. This neuronal regeneration assay has been adapted to run on a confocal microscope. Quantitative fluorescence is then used to measure a compounds ability to regenerate neuronal firing.The membrane potential dye di-8-ANEPPS was selected for these experiments. Di-8- ANEPPS is internalized slowly, has a high signal to noise ratio (40:1), has a linear fluorescent response to change in voltage.

Error analysis of Cm measurement under the whole-cell patch-clamp recording

2010 ◽  
Vol 185 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Hao Zhang ◽  
Anlian Qu ◽  
Jie Luo ◽  
Jie Luo
Keyword(s):  
Patch Clamp ◽  
Error Analysis ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp

scholarly journals Electrophysiological Properties of Substantia Gelatinosa Neurons in the Preparation of a Slice of Middle-Aged Rat Spinal Cord

2021 ◽  
Vol 13 ◽  
Author(s):  
Yang Li ◽  
Shanchu Su ◽  
Jiaqi Yu ◽  
Minjing Peng ◽  
Shengjun Wan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Patch Clamp ◽  
Molecular Mechanisms ◽  
Substantia Gelatinosa ◽  
Membrane Properties ◽  
Middle Aged ◽  
Patch Clamp Recording ◽  
Electrophysiological Properties ◽  
Glutamatergic Neurons ◽  
Single Spike

A patch-clamp recording in slices generated from the brain or the spinal cord has facilitated the exploration of neuronal circuits and the molecular mechanisms underlying neurological disorders. However, the rodents that are used to generate the spinal cord slices in previous studies involving a patch-clamp recording have been limited to those in the juvenile or adolescent stage. Here, we applied an N-methyl-D-glucamine HCl (NMDG-HCl) solution that enabled the patch-clamp recordings to be performed on the superficial dorsal horn neurons in the slices derived from middle-aged rats. The success rate of stable recordings from substantia gelatinosa (SG) neurons was 34.6% (90/260). When stimulated with long current pulses, 43.3% (39/90) of the neurons presented a tonic-firing pattern, which was considered to represent γ-aminobutyric acid-ergic (GABAergic) signals. Presumptive glutamatergic neurons presented 38.9% (35/90) delayed and 8.3% (7/90) single-spike patterns. The intrinsic membrane properties of both the neuron types were similar but delayed (glutamatergic) neurons appeared to be more excitable as indicated by the decreased latency and rheobase values of the action potential compared with those of tonic (GABAergic) neurons. Furthermore, the glutamatergic neurons were integrated, which receive more excitatory synaptic transmission. We demonstrated that the NMDG-HCl cutting solution could be used to prepare the spinal cord slices of middle-aged rodents for the patch-clamp recording. In combination with other techniques, this preparation method might permit the further study of the functions of the spinal cord in the pathological processes that occur in aging-associated diseases.

Patch Clamp Recording

2012 ◽  
pp. 95-103
Author(s):  
Neil Bannister ◽  
Phil Langton
Keyword(s):  
Patch Clamp ◽  
Patch Clamp Recording

Patch-Clamp Recording and RT-PCR on Single Cells

2003 ◽  
pp. 193-232 ◽  
Author(s):  
Bertrand Lambolez ◽  
Etienne Audinat ◽  
Pascal Bochet ◽  
Jean Rossier
Keyword(s):  
Patch Clamp ◽  
Single Cells ◽  
Rt Pcr ◽  
Patch Clamp Recording

Analyzing Ion Permeation in Channels and Pumps Using Patch-Clamp Recording

2015 ◽  
pp. 51-88 ◽  
Author(s):  
Andrew J. Moorhouse ◽  
Trevor M. Lewis ◽  
Peter H. Barry
Keyword(s):  
Patch Clamp ◽  
Ion Permeation ◽  
Patch Clamp Recording

Patch-Clamp Recording

2009 ◽  
pp. 20-35
Author(s):  
W. Otto Friesen ◽  
Jonathon A. Friesen
Keyword(s):  
Patch Clamp ◽  
Patch Clamp Recording
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