Use of cholera toxin subunit B to label neural projections to lower urinary tract organs v1

2021 ◽  
Author(s):  
Janet R Keast ◽  
Peregrine B Osborne ◽  
John-Paul Fuller-Jackson
Keyword(s):  
Urinary Tract ◽  
Cholera Toxin ◽  
Lower Urinary Tract ◽  
Animal Experimentation ◽  
Female Rat ◽  
Cholera Toxin Subunit B ◽  
Autonomic Neurons ◽  
Subunit B ◽  
Operative Monitoring ◽  
Lower Urinary

This protocol is used to visualise sensory and autonomic neurons innervating organs of the lower urinary tract in an experimental adult male or female rat. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia, surgical environment, and post-operative monitoring and care.

Immunohistochemical labelling of lower urinary tract afferents in spinal cord v1

2021 ◽  
Author(s):  
Janet R Keast ◽  
Peregrine B Osborne ◽  
John-Paul Fuller-Jackson
Keyword(s):  
Spinal Cord ◽  
Urinary Tract ◽  
Cholera Toxin ◽  
Lower Urinary Tract ◽  
Double Labelling ◽  
Toxin B ◽  
Lumbosacral Spinal Cord ◽  
Cholera Toxin Subunit B ◽  
Subunit B ◽  
Lower Urinary

This protocol is used for immunohistochemical visualisation of cholera toxin subunit B within afferents innervating the lower urinary tract in cryosections of rat lumbosacral spinal cord. Free-floating sections are processed in a double labelling protocol to distinguish regions of innervation by these afferents. Cholera toxin B antibody [lower urinary tract afferents] Choline acetyltransferase antibody [preganglionic autonomic neurons and motoneurons]

Immunolabelling and clearing of intact spinal cord for visualization of lower urinary tract afferents v1

2021 ◽  
Author(s):  
Janet R Keast ◽  
Peregrine B Osborne ◽  
John-Paul Fuller-Jackson
Keyword(s):  
Spinal Cord ◽  
Urinary Tract ◽  
Lower Urinary Tract ◽  
Light Sheet ◽  
Lumbosacral Spinal Cord ◽  
Cholera Toxin Subunit B ◽  
Autonomic Neurons ◽  
Subunit B ◽  
Clearing Method ◽  
Lower Urinary

The whole-mount immunolabeling and clearing method (iDISCO) was used to visualize cholera toxin subunit B-labelled lower urinary tract afferents in the lumbosacral spinal cord of the rat. Imaging of spinal cord was performed on a light sheet microscope with a 12x lens. Concurrently, choline acetyltransferase identified preganglionic autonomic neurons and motoneurons within the spinal cord, which were used to confirm the rostrocaudal location of afferents.

Regional heterogeneity in expression of the sphingosine-1-phosphate pathway in the female rat lower urinary tract

2009 ◽  
Vol 200 (5) ◽  
pp. 576.e1-576.e7 ◽  
Author(s):  
Katherine S. Sandhu ◽  
Rowena G. Chua ◽  
Xinhua Zhang ◽  
Nirmala Devi Kanika ◽  
Sarah A. Collins ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Lower Urinary Tract ◽  
Female Rat ◽  
Regional Heterogeneity ◽  
Sphingosine 1 Phosphate ◽  
Lower Urinary

scholarly journals Verification and Defined Dosage of Sodium Pentobarbital for a Urodynamic Study in the Possibility of Survival Experiments in Female Rat

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Sheng-Fei Xu ◽  
Guang-Hui Du ◽  
Kuerbanjiang Abulikim ◽  
Peng Cao ◽  
Hui-bing Tan
Keyword(s):  
Urinary Tract ◽  
Lower Urinary Tract ◽  
Female Rats ◽  
Sodium Pentobarbital ◽  
Female Rat ◽  
Leak Point Pressure ◽  
Lower Urinary Tract Function ◽  
Point Pressure ◽  
Tract Function ◽  
Lower Urinary

Objectives. To evaluate the effects of pentobarbital dosages on lower urinary tract function and to define an appropriate dosage of sodium pentobarbital that would be suitable for urodynamic studies in which recovery from anesthesia and long term survive were needed for subsequent experiment. Methods. Twenty-four 8-week-old, female, virgin, Sprague-Dawley rats (200-250 g) were used in this study. Rats in study groups received gradient doses of pentobarbital intraperitoneally, and those in the control group received urethane intraperitoneally. External urethral sphincter electromyography (EUS-EMG) was recorded simultaneously during cystometry and leak point pressure tests. The toe-pinch reflex was used to determine the level of anesthesia. Results. Micturition was normally induced in both the urethane group and 32 mg/kg pentobarbital group. However, in groups of 40 mg/kg or 36 mg/kg pentobarbital, micturition failed to be induced; instead, nonvoiding contractions accompanied by EUS-EMG tonic activity were observed. There were no significant differences in leak point pressure or EUS-EMG amplitude or frequency between the urethane and 32 mg/kg pentobarbital groups. Conclusions. This study confirmed significant dose-dependent effects of pentobarbital on lower urinary tract function and 32 mg/kg pentobarbital as an appropriate dosage for recovery urodynamic testing, which enable the achievement of expected essential micturition under satisfactory anesthesia in female rats.

Sign in / Sign up

Export Citation Format

Share Document