Regional heterogeneity and unexpectedly high abundance of Cooperia punctata in beef cattle at a northern latitude revealed by ITS-2 rDNA nemabiome metabarcoding

Background The species composition of cattle gastrointestinal nematode (GIN) communities can vary greatly between regions. Despite this, there is remarkably little large-scale surveillance data for cattle GIN species which is due, at least in part, to a lack of scalable diagnostic tools. This lack of regional GIN species-level data represents a major knowledge gap for evidence-based parasite management and assessing the status and impact of factors such as climate change and anthelmintic drug resistance. Methods This paper presents a large-scale survey of GIN in beef herds across western Canada using ITS-2 rDNA nemabiome metabarcoding. Individual fecal samples were collected from 6 to 20 randomly selected heifers (n = 1665) from each of 85 herds between September 2016 and February 2017 and 10–25 first season calves (n = 824) from each of 42 herds between November 2016 and February 2017. Results Gastrointestinal nematode communities in heifers and calves were similar in Alberta and Saskatchewan, with Ostertagia ostertagi and Cooperia oncophora being the predominant GIN species in all herds consistent with previous studies. However, in Manitoba, Cooperia punctata was the predominant species overall and the most abundant GIN species in calves from 4/8 beef herds. Conclusions This study revealed a marked regional heterogeneity of GIN species in grazing beef herds in western Canada. The predominance of C. punctata in Manitoba is unexpected, as although this parasite is often the predominant cattle GIN species in more southerly latitudes, it is generally only a minor component of cattle GIN communities in northern temperate regions. We hypothesize that the unexpected predominance of C. punctata at such a northerly latitude represents a range expansion, likely associated with changes in climate, anthelmintic use, management, and/or animal movement. Whatever the cause, these results are of practical concern since C. punctata is more pathogenic than C. oncophora, the Cooperia species that typically predominates in cooler temperate regions. Finally, this study illustrates the value of ITS-2 rDNA nemabiome metabarcoding as a surveillance tool for ruminant GIN parasites. Graphical Abstract

China’s Carbon Emission Trading Pilot Policy and China’s Export Technical Sophistication: Based on DID Analysis

This paper takes China’s carbon emission trading pilot policy as a quasi-natural experiment, and adopts a difference-in-difference approach and data from 30 provinces in China from 2008 to 2016 to empirically study the influence of this policy on China’s export technical sophistication. The empirical analysis revealed that the policy can generate a Porter effect and progressively promote China’s export technical sophistication by reinforcing carbon productivity. By analyzing the regional heterogeneity and influence channels, the policy is found to work better in the central-western region than in the eastern region. The reason for this finding is that the policy has brought innovation offset effects to the central-western region and increased carbon productivity, but the policy has not improved carbon productivity in the eastern region. By studying the effect of three measures of policy implementation on export technical sophistication, we found that restricting carbon emission quotas distributed to participating enterprises is necessary. In addition, we found that the financial punishment method for non-performance is advantageous to the enhancement of export technical sophistication. These research conclusions can provide directions and policy recommendations for upgrading the emissions trading market, as well as a learning case and some experience for countries that have not yet established carbon trading markets.

Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats

Pre-existing Ca2+ handling abnormalities constitute the arrhythmogenic substrate in patients developing postoperative atrial fibrillation (POAF), a common complication after cardiac surgery. Postoperative interleukin (IL)-6 levels are associated with atrial fibrosis in several animal models of POAF, contributing to atrial arrhythmias. Here, we hypothesize that IL-6-mediated-Ca2+ handling abnormalities contribute to atrial fibrillation (AF) in sterile pericarditis (SP) rats, an animal model of POAF. SP was induced in rats by dusting atria with sterile talcum powder. Anti-rat-IL-6 antibody (16.7 μg/kg) was administered intraperitoneally at 30 min after the recovery of anesthesia. In vivo electrophysiology, ex vivo optical mapping, western blots, and immunohistochemistry were performed to elucidate mechanisms of AF susceptibility. IL-6 neutralization ameliorated atrial inflammation and fibrosis, as well as AF susceptibility in vivo and the frequency of atrial ectopy and AF with a reentrant pattern in SP rats ex vivo. IL-6 neutralization reversed the prolongation and regional heterogeneity of Ca2+ transient duration, relieved alternans, reduced the incidence of discordant alternans, and prevented the reduction and regional heterogeneity of the recovery ratio of Ca2+ transient. In agreement, western blots showed that IL-6 neutralization reversed the reduction in the expression of ryanodine receptor 2 (RyR2) and phosphorylated phospholamban. Acute IL-6 administration to isolated rat hearts recapitulated partial Ca2+ handling phenotype in SP rats. In addition, intraperitoneal IL-6 administration to rats increased AF susceptibility, independent of fibrosis. Our results reveal that IL-6-mediated-Ca2+ handling abnormalities in SP rats, especially RyR2-dysfunction, independent of IL-6-induced-fibrosis, early contribute to the development of POAF by increasing propensity for arrhythmogenic alternans.

Spatiotemporal Variations in Meteorological Disasters and Vulnerability in China During 2001–2020

Based on the Yearbook of Meteorological Disasters in China, we analyzed the spatiotemporal variations in major meteorological disaster (MD) losses at the provincial scale during 2001–2020 to determine the spatiotemporal variations in MDs and vulnerability in China. Our results suggest that the impacts of MDs, including floods, droughts, hail and strong winds (HSs), low temperature and frosts (LTFs), and typhoons, have been substantial in China. MDs in China affect an average of 316.3 million people and 34.3 million hectares of crops each year, causing 1,739 deaths and costing 372.3 billion yuan in direct economic losses (DELs). Floods and droughts affected more of the population in China than the other MDs. Fatalities and DELs were mainly caused by floods, and the affected crop area was mainly impacted by drought. The national average MD losses decreased significantly, except for DELs. The trends in the affected population and crop area were mainly caused by droughts, and the trends in fatalities and DELs were dominated by floods. Floods and typhoons showed increasing influence in the last two decades relative to other disasters. The annual mean and long-term trends in MD losses exhibited regional heterogeneity and were subject to different dominant hazards in different regions. The disaster losses and their trends in southeastern China were mainly attributed to typhoons. The affected population, crop area, and DELs were all significantly and positively correlated with exposure. The vulnerability of the population, crops, and economy tended to decrease. Economic development reduced the vulnerability of the population and economy but showed no significant influence on the vulnerability of crops. Our findings suggest that more focus should be placed on the impacts of floods and typhoons and that socioeconomic development has an important influence on the vulnerability of the population and economy. These results provide a foundation for designing effective disaster prevention and mitigation measures.

U.S. regional differences in physical distancing: Evaluating racial and socioeconomic divides during the COVID-19 pandemic

Health varies by U.S. region of residence. Despite regional heterogeneity in the outbreak of COVID-19, regional differences in physical distancing behaviors over time are relatively unknown. This study examines regional variation in physical distancing trends during the COVID-19 pandemic and investigates variation by race and socioeconomic status (SES) within regions. Data from the 2015–2019 five-year American Community Survey were matched with anonymized location pings data from over 20 million mobile devices (SafeGraph, Inc.) at the Census block group level. We visually present trends in the stay-at-home proportion by Census region, race, and SES throughout 2020 and conduct regression analyses to examine these patterns. From March to December, the stay-at-home proportion was highest in the Northeast (0.25 in March to 0.35 in December) and lowest in the South (0.24 to 0.30). Across all regions, the stay-at-home proportion was higher in block groups with a higher percentage of Blacks, as Blacks disproportionately live in urban areas where stay-at-home rates were higher (0.009 [CI: 0.008, 0.009]). In the South, West, and Midwest, higher-SES block groups stayed home at the lowest rates pre-pandemic; however, this trend reversed throughout March before converging in the months following. In the Northeast, lower-SES block groups stayed home at comparable rates to higher-SES block groups during the height of the pandemic but diverged in the months following. Differences in physical distancing behaviors exist across U.S. regions, with a pronounced Southern and rural disadvantage. Results can be used to guide reopening and COVID-19 mitigation plans.

Whether Urbanization Has Intensified the Spread of Infectious Diseases—Renewed Question by the COVID-19 Pandemic

The outbreak of the COVID-19 epidemic has triggered adiscussion of the relationship between urbanization and the spread of infectious diseases. Namely, whether urbanization will exacerbate the spread of infectious diseases. Based on 31 provincial data from 2002 to 2018 in China, the impact of urbanization on the spread of infectious diseases from the dimensions of “population” and “land” is analyzed in this paper by using the GMM (generalized method of moments) model. The empirical study shows that the population increase brought by urbanization does not aggravate the spread of infectious diseases. On the contrary, urban education, employment and entrepreneurship, housing, medical and health care, and other basic public services brought by population urbanization can help reduce the risk of the spread of infectious diseases. The increasing density of buildings caused by land urbanization increases the risk of the spread of infectious diseases. Moreover, the impact of urbanization on the spread of infectious diseases has regional heterogeneity. Therefore, the prevention and control of disease play a crucial role.

Tradeoff in the supply and demand for CO 2 dominates the divergence of net photosynthesis rates of functional plants in alpine ecosystems

As regional heterogeneity on the Qinghai Tibetan Plateau (QTP), the “greening rate” between alpine steppe in the west and alpine meadow ecosystems in the east is difference during the past several decades. To investigate the difference, the net photosynthetic rate (An) and the supply (mesophyll conductance ( g), stomatal conductance ( g)) and demand (the maximum rates of Rubisco carboxylase activity ( V) and photosynthetic electron transport ( J)) for CO of three plants functional types (PFTs) were measured. Other functional traits and influencing factors were compared among ecosystems along the altitudinal gradients of QTP. The An of the PFTs was simulated under potential future conditions. At high altitudes, grass was found to maintain a relatively stable An by decreasing V, J, and g, while slightly increasing g, compared with that at a low altitude. The An of sedge and shrubs increased with rising V, J and g and g values, resulting in a large increment in the An at low altitudes. Grass seemed to be less sensitive to the environment by reducing the supply of and holding onto CO , while sedge and shrub increased both. Grass and sedge should be divided into two PFTs rather than remaining as one based on their opposite physiological and morphological functions in response to climate change. The ecosystem at 3600 m was transitional. C was likely to be a more dominant factor than T in affecting the An of grass. The order of rising An in PFTs was shrub > sedge > grass and the An of alpine meadow was found to increase more under the two future climate scenarios.