Recipe for standard BG-11 media v1

Stanier RY, Deruelles J, Rippka R, Herdman M, Waterbury JB: Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 1979, 111:1–61. Recipes for standard and alternative BG11 for culturing freshwater cyanobacteria, such as Synechocystis sp. PCC 6803, as described. Media is usually not suitable for marine cyanobacteria. Final Concentration of Medium. CaCl2*2 H2O 0.036 g/L Citric acid 0.006 g/L NaNO3 1.4958 g/L MgSO4* 7 H2O 0.0749 g/L 0.25M Na2EDTA (pH 8) 0.0056 mL/L Na2CO3 20 µg/ml Fe(III) Ammonium citrate 6 µg/ml K2HPO4 * 3H2O 30 µg/ml TES Buffer (pH 8) 10 mM H3BO3 2.86 mg/L MnCl2 * 4 H2O 1.81 mg/L ZnSO4 * 7 H2O 0.222 mg/L Na2MoO4 * 2 H2O 0.390 mg/L Co(NO3)2 *6 H2O 0.049 mg/L (CuSO4 * 5 H2O 0.079 mg/L if required)

A study of the impact of mazie SBE I on the [alpha]-polyglucan produced in Synechocystis sp. strain PCC 6803

10.31274/rtd-180813-17053 ◽

2007 ◽

Author(s):

Shayani Deborah Nesaranjani Pieris

Keyword(s):

The Impact ◽

Synechocystis Sp ◽

Faculty Opinions recommendation of ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1160026.620293 ◽

2009 ◽

Author(s):

Keith Chater

Keyword(s):

Sigma Factor ◽

Mg Chelatase ◽

Synechocystis Sp ◽

Faculty Opinions recommendation of The cyanobacterial homologue of HCF136/YCF48 is a component of an early photosystem II assembly complex and is important for both the efficient assembly and repair of photosystem II in Synechocystis sp. PCC 6803.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.7466956.7759054 ◽

2011 ◽

Author(s):

Dario Leister

Keyword(s):

Photosystem Ii ◽

Synechocystis Sp ◽

Chlorophyll in a Synechocystis sp. PCC 6803 mutant without photosystem I and photosystem II core complexes. Evidence for peripheral antenna chlorophylls in cyanobacteria.

10.1016/s0021-9258(17)36733-9 ◽

1994 ◽

Vol 269 (19) ◽

pp. 13904-13910

Author(s):

G. Shen ◽

W.F. Vermaas

Keyword(s):

Photosystem Ii ◽

Photosystem I ◽

Antenna Chlorophylls ◽

Synechocystis Sp ◽

Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803.

10.1016/s0021-9258(19)77887-9 ◽

1988 ◽

Vol 263 (33) ◽

pp. 17658-17662

Author(s):

P Reilly ◽

J D Hulmes ◽

Y C Pan ◽

N Nelson

Keyword(s):

Molecular Cloning ◽

Photosystem I ◽

Cloning And Sequencing ◽

Gene Encoding ◽

Synechocystis Sp ◽

Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

10.1016/s0021-9258(18)99134-9 ◽

1991 ◽

Vol 266 (17) ◽

pp. 11111-11115

Author(s):

M. Ikeuchi ◽

B. Eggers ◽

G.Z. Shen ◽

A. Webber ◽

J.J. Yu ◽

...

Keyword(s):

Photosystem Ii ◽

Synechocystis Sp ◽

Molecular cloning and targeted mutagenesis of the gene psaF encoding subunit III of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803.

10.1016/s0021-9258(18)54902-4 ◽

1991 ◽

Vol 266 (30) ◽

pp. 20146-20151 ◽

Cited By ~ 1

Author(s):

P.R. Chitnis ◽

D. Purvis ◽

N. Nelson

Keyword(s):

Molecular Cloning ◽

Photosystem I ◽

Targeted Mutagenesis ◽

Subunit Iii ◽

Synechocystis Sp ◽

A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803

Scientific Reports ◽

10.1038/s41598-020-78475-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Anushree Bachhar ◽

Jiri Jablonsky

Keyword(s):

In Silico Analysis ◽

Bioinformatic Analysis ◽

Phosphoglycerate Mutase ◽

Phosphoketolase Pathway ◽

Multi Level ◽

Silico Analysis ◽

Insight Into ◽

Synechocystis Sp ◽

AbstractPhosphoketolase (PKET) pathway is predominant in cyanobacteria (around 98%) but current opinion is that it is virtually inactive under autotrophic ambient CO2 condition (AC-auto). This creates an evolutionary paradox due to the existence of PKET pathway in obligatory photoautotrophs. We aim to answer the paradox with the aid of bioinformatic analysis along with metabolic, transcriptomic, fluxomic and mutant data integrated into a multi-level kinetic model. We discussed the problems linked to neglected isozyme, pket2 (sll0529) and inconsistencies towards the explanation of residual flux via PKET pathway in the case of silenced pket1 (slr0453) in Synechocystis sp. PCC 6803. Our in silico analysis showed: (1) 17% flux reduction via RuBisCO for Δpket1 under AC-auto, (2) 11.2–14.3% growth decrease for Δpket2 in turbulent AC-auto, and (3) flux via PKET pathway reaching up to 252% of the flux via phosphoglycerate mutase under AC-auto. All results imply that PKET pathway plays a crucial role under AC-auto by mitigating the decarboxylation occurring in OPP pathway and conversion of pyruvate to acetyl CoA linked to EMP glycolysis under the carbon scarce environment. Finally, our model predicted that PKETs have low affinity to S7P as a substrate.

Synechocystis sp. PCC 6803 mutant lacking both photosystems exhibits strong carotenoid-induced quenching of phycobilisome fluorescence

FEBS Letters ◽

10.1016/j.febslet.2011.01.013 ◽

2011 ◽

Vol 585 (3) ◽

pp. 585-589 ◽

Cited By ~ 22

Author(s):

Marina G. Rakhimberdieva ◽

Fedor I. Kuzminov ◽

Irina V. Elanskaya ◽

Navassard V. Karapetyan

Keyword(s):

Synechocystis Sp ◽

Volume 1A, Symposia: Turbomachinery Flow Simulation and Optimization; Applications in CFD; Bio-Inspired and Bio-Medical Fluid Mechanics; CFD Verification and Validation; Development and Applications of Immersed Boundary Methods; DNS, LES and Hybrid RANS/LES Methods; Fluid Machinery; Fluid-Structure Interaction and Flow-Induced Noise in Industrial Applications; Flow Applications in Aerospace; Active Fluid Dynamics and Flow Control — Theory, Experiments and Implementation ◽

Motion of Active Fluids: Diffusion Dynamics of Cyanobacteria

10.1115/fedsm2016-7526 ◽

2016 ◽

Author(s):

Thomas Vourc’h ◽

Julien Léopoldès ◽

Annick Méjean ◽

Hassan Peerhossaini

Keyword(s):

Time Lapse ◽

Global Behavior ◽

Diffusion Dynamics ◽

Mean Squared Displacement ◽

Terrestrial Environments ◽

The Mean ◽

Micro Organisms ◽

Synechocystis Sp ◽

Time Lapse Video ◽

Cyanobacteria are photosynthetic micro-organisms colonizing all aquatic and terrestrial environments. The motility of such living micro-organisms should make their diffusion distinct from typical Brownian motion. This diffusion can be investigated in terms of global behavior (Fickian or not) and in terms of displacement probabilities, which provide more detail about the motility process. Using cyanobacterium Synechocystis sp. PCC 6803 as the model micro-organism, we carry out time-lapse video microscopy to track and analyze the bacteria’s trajectories, from which we compute the mean-squared displacement (MSD) and the distribution function of displacement probabilities. We find that the motility of Synechocystis sp. PCC 6803 is intermittent: high-motility “run” phases are separated by low-motility “tumble” phases corresponding to trapped states. However, this intermittent motility leads to a Fickian diffusive behavior, as shown by the evolution of the MSD with time.