scholarly journals Clinico-hematological profile and morphological features on bone marrow aspirate in visceral leishmaniasis from non-endemic region

2019 ◽  
Vol 5 (6) ◽  
pp. 408-412
Author(s):  
Dr. Arvind Kumar Gupta ◽  
◽  
Dr. Harish Chandra ◽  
Dr. Vandana Bharati ◽  
Dr. Neha Singh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Bone Marrow Aspirate ◽  
Morphological Features ◽  
Endemic Region ◽  
Hematological Profile

scholarly journals Visceral Leishmaniasis with Associated Common, Uncommon, and Atypical Morphological Features on Bone Marrow Aspirate Cytology in Nonendemic Region

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Harish Chandra ◽  
Smita Chandra ◽  
Rajeev Mohan Kaushik
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Aspirate ◽  
Correct Diagnosis ◽  
Giant Cells ◽  
Morphological Features ◽  
Endemic Region ◽  
Common Presentation ◽  
Atypical Features ◽  
Cytological Features ◽  
The Common

Objectives. The present study was conducted to categorise the morphological features on bone marrow aspirate cytology into common, uncommon, and atypical features in a nonendemic region which would be helpful in clinching an early and correct diagnosis especially in clinically unsuspected cases.Methods. The morphological features on bone marrow were categorized into common, uncommon, and atypical in cases of leishmaniasis from non endemic region.Results. Out of total 27 cases, 77.7% were residents of places at the height of 500 m or above and fever was the most common presentation followed by hepatosplenomegaly. Plasmacytosis, hemophagocytosis were the common cytological features while dysmyelopoiesis, presence of leishmania bodies in nonhistiocytic cells, and granuloma with necrosis were uncommon features. Aggregates of LD bodies in form of ring, floret, or strap shapes along with giant cells constitute the atypical morphological features.Conclusion. The knowledge of common, uncommon, and atypical features on bone marrow aspirate cytology is helpful in clinching an early and correct diagnosis of leishmaniasis especially in non endemic areas where clinical suspicion is low. These features will guide the pathologist for vigilant search of LD bodies in the marrow for definite diagnosis and thus will also be helpful in preventing unnecessary workups.

scholarly journals An acute presentation of haemophagocytic lymphohistiocytosis due to visceral leishmaniasis in a British adult returning traveller

2019 ◽  
Vol 18 (3) ◽  
pp. 184-188
Author(s):  
Anastasia A Theodosiou ◽  
◽  
Hwai Jing Hiew ◽  
Christina Petridou ◽  
◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Bone Marrow Aspirate ◽  
Caucasian Woman ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Uk ◽  
Very High

A 62-year old British Caucasian woman normally resident in Spain presented with fever and pancytopaenia after returning to the UK. Her symptoms persisted despite broad-spectrum antibiotics, and she gradually became confused, hypotensive and progressively more pancytopaenic. Imaging demonstrated hepatosplenomegaly, and a bone marrow aspirate confirmed a diagnosis of haemophagocytic lymphohistiocytosis (HLH). Bone marrow polymerase chain reaction (PCR) and blood serology were both positive for Leishmania donovani, consistent with visceral leishmaniasis (VL). Following treatment with dexamethasone and amphotericin, she improved clinically and biochemically, and was able to return to Spain. Fever in the returning traveller is a common acute medical presentation. Although HLH and VL are rare diagnoses, both carry a very high mortality rate if undiagnosed and untreated.

Diverse liver morphology in two cases of visceral leishmaniasis: Same face, different expressions

2021 ◽  
pp. 004947552110381
Author(s):  
Shashi Dhawan ◽  
Satyender S Dharamdesani
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Bone Marrow Examination ◽  
The Other ◽  
Endemic Region ◽  
Travel History ◽  
Liver Morphology ◽  
Liver Biopsies

Two cases of visceral leishmaniasis are presented; one patient was from an endemic region and visceral leishmaniasis was suspected clinically, while the other was from a non-endemic region and it was not suspected clinically. Bone marrow examination was negative in both cases and both showed different morphological pictures in the liver biopsies. These are discussed. Importance of travel history is also highlighted even if it is in distant past.

scholarly journals Diagnosing visceral leishmaniasis and HIV/AIDS co-infection: a case series study in Pernambuco, Brazil

2012 ◽  
Vol 54 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Aracele Tenório de Almeida e Cavalcanti ◽  
Zulma Medeiros ◽  
Fábio Lopes ◽  
Luiz Dias de Andrade ◽  
Valéria de Melo Ferreira ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Bone Marrow Aspirate ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Chain Reaction ◽  
Dipstick Test ◽  
Polymerase Chain

HIV/AIDS-associated visceral leishmaniasis may display the characteristics of an aggressive disease or without specific symptoms at all, thus making diagnosis difficult. The present study describes the results of diagnostic tests applied to a series of suspected VL cases in HIV-infected/AIDS patients admitted in referral hospitals in Pernambuco, Brazil. From a total of 14 eligible patients with cytopenias and/or fever of an unknown etiology, and indication of bone marrow aspirate, 10 patients were selected for inclusion in the study. Diagnosis was confirmed by the following examinations: Leishmania detection in bone marrow aspirate, direct agglutination test, indirect immunofluorescence, rK39 dipstick test, polymerase chain reaction and latex agglutination test. Five out of the ten patients were diagnosed with co-infection. A positive direct agglutination test was recorded for all five co-infected patients, the Leishmania detection and latex agglutination tests were positive in four patients, the rK39 dipstick test in three, the indirect immunofluorescence in two and a positive polymerase chain reaction was recorded for one patient. This series of cases was the first to be conducted in Brazil using this set of tests in order to detect co-infection. However, no consensus has thus far been reached regarding the most appropriate examination for the screening and monitoring of this group of patients.

Visceral Leishmaniasis (Kala-Azar) in bone marrow aspirate

2002 ◽  
Vol 68 (6) ◽  
pp. 400-400 ◽  
Author(s):  
T Economopoulos ◽  
G Giannopoulos ◽  
S Raptis
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Bone Marrow Aspirate ◽  
Kala Azar

scholarly journals Epistaxis in Visceral Leishmaniasis with Hematological Correlation

2012 ◽  
Vol 2012 ◽  
pp. 1-3 ◽  
Author(s):  
B. Sigdel ◽  
S. Bhandary ◽  
S. Rijal
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Study Design ◽  
Endemic Area ◽  
Age Group ◽  
Peripheral Smear ◽  
Cross Sectional ◽  
Kala Azar ◽  
Hematological Profile

Objective. To study the prevalence of epistaxis in visceral leismaniasis and its correlation with hematological profile.Methods. Out of 80 diagnosed cases of visceral leishmaniasis, 19 patients with epistaxis were included in the study. Diagnosis was made by Rk-39 from peripheral smear and LD bodies from bone marrow. Before starting anti-kala-azar treatment, nasal examination findings and hematological profile were noted.Study Design. Prospective cross-sectional hospital-based study.Results. Epistaxis was found in the age group of 7–66 years. Epistaxis was observed in 19 (23.8%) cases. One patient died because of epistaxis and neck hematoma.Conclusion. Epistaxis is a common ENT finding in endemic area of visceral leishmaniasis like our case.

DETECTION OF LEISHMANIA INFECTION IN STRAY DOGS IN HUMAN LEISHMANIASIS ENDEMIC AREA IN MYMENSINGH DISTRICT WITH ITS POSSIBLE PUBLIC HEALTH SIGNIFICANCE IN BANGLADESH

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
M. A. Hossen
Keyword(s):  
Visceral Leishmaniasis ◽  
Asymptomatic Carrier ◽  
Asymptomatic Infection ◽  
Reservoir Host ◽  
Sand Fly ◽  
Leishmania Infection ◽  
Endemic Region ◽  
Stray Dogs ◽  
Human Leishmaniasis ◽  
Strip Test

Background: Leishmaniasis is primarily caused by two species of Leishmania (L. donovani and L. infantum) of which clinical infection with L. infantum has been recognized in both humans and dogs as zoonotic disease with dogs as the main reservoir hosts in the Mediterranean, the Middle East, Asia and South America. Although L. donovani has been associated with both clinical and asymptomatic infection in humans but it is still associated with asymptomatic infection in dogs in Indian sub-continent without any evidence of zoonotic infection. Objectives: The objective of this research was to investigate the potentiality of dog as reservoir host for visceral leishmaniasis in the human leishmaniasis endemic regions in Bangladesh. Materials and Methods: A total of 20 stray dogs in the human VL endemic areas of Mymensingh district were captured for the detection VL during the period of November 2010 to May 2011. The dipstick test rK39 (Bios International; n = 20), Giemsa’s stained impression smears of liver and spleen (n = 6) and PCR with the tissue of liver and spleen (n = 6) were tested as per manufacturer instructions and conventional standard methods. Results: Out of 20 stray dogs examined, 4 (20.0%) were positive for L. donovani infection with rK39 strip test. Of the six randomly selected dogs tested with Modified Giemsa’s stained of impression smears of spleen and liver showed 2 (33.33%) positive whereas PCR technique detected 5 (83.33%) positive for L. donovani. Results of PCR showed 145bp amplicon, specific for L. donovani infection in 83.33% stray dogs. Conclusions: This study reveals that a high percentage of L. donovani asymptomatic carrier infections occur in dogs and evidence indicates that dogs and humans may potentially serve as a source of infection to sand fly vectors and accordingly dogs can be recognized as a probable animal reservoir for the Leishmania infection in the endemic region in Bangladesh. However, further studies are required to determine the ability of dogs to transmit the L. donovani to the vector sand fly in nature and its evidence on ‘One Health’ perspectives. Keywords: Visceral leishmaniasis, Endemic region, Stray dogs, rK39 strip test, Giemsa’s stained liver and spleen impression smears, PCR, Reservoir host, Mymensingh

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