scholarly journals Efflux Pump Mediated Antibiotic Resistance in Clinical Isolates of Helicobacter Pylori From South West Nigeria

2020 ◽  
Vol 9 (4) ◽  
pp. 3283-3289
Author(s):  
Tolulope Funbi Jolaiya ◽  
◽  
Muinah Adenike Fowora ◽  
Charles Onyekwere ◽  
Rose Ugiagbe ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Clinical Isolates ◽  
South West

scholarly journals Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru

2017 ◽  
Vol Volume 10 ◽  
pp. 85-90 ◽  
Author(s):  
Kevin F Boehnke ◽  
Manuel Valdivieso ◽  
Alejandro Bussalleu ◽  
Rachael Sexton ◽  
Kathryn C Thompson ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Clinical Isolates

scholarly journals Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates

Toxins ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 473 ◽  
Author(s):  
Kartika Afrida Fauzia ◽  
Muhammad Miftahussurur ◽  
Ari Fahrial Syam ◽  
Langgeng Agung Waskito ◽  
Dalla Doohan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Crystal Violet ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Clinical Isolates ◽  
Resistance Phenotype ◽  
Resistance To Antibiotics ◽  
Inhibition Concentration ◽  
H Pylori

We evaluated biofilm formation of clinical Helicobacter pylori isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to measure the planktonic susceptibility of 101 H. pylori strains. Biofilms were quantified by the crystal violet method. The minimum biofilm eradication concentration (MBEC) was obtained by measuring the survival of bacteria in a biofilm after exposure to antibiotics. The majority of the strains formed a biofilm (93.1% (94/101)), including weak (75.5%) and strong (24.5%) biofilm-formers. Planktonic resistant and sensitive strains produced relatively equal amounts of biofilms. The resistance proportion, shown by the MBEC measurement, was higher in the strong biofilm group for all antibiotics compared to the weak biofilm group, especially for clarithromycin (p = 0.002). Several cases showed sensitivity by the MIC measurement, but resistance according to the MBEC measurements (amoxicillin, 47.6%; tetracycline, 57.1%; clarithromycin, 19.0%; levofloxacin, 38.1%; and metronidazole 38.1%). Thus, biofilm formation may increase the survival of H. pylori and its resistance to antibiotics. Biofilm-related antibiotic resistance should be evaluated with antibiotic susceptibility.

scholarly journals Alkylaminoquinolines inhibit the bacterial antibiotic efflux pump in multidrug-resistant clinical isolates

2003 ◽  
Vol 376 (3) ◽  
pp. 801-805 ◽  
Author(s):  
Monique MALLÉA ◽  
Abdallah MAHAMOUD ◽  
Jacqueline CHEVALIER ◽  
Sandrine ALIBERT-FRANCO ◽  
Pierre BROUANT ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Microbial Pathogens ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
High Level ◽  
Antibiotic Efflux

Over the last decade, MDR (multidrug resistance) has increased worldwide in microbial pathogens by efflux mechanisms, leading to treatment failures in human infections. Several Gram-negative bacteria efflux pumps have been described. These proteinaceous channels are capable of expelling structurally different drugs across the envelope and conferring antibiotic resistance in various bacterial pathogens. Combating antibiotic resistance is an urgency and the blocking of efflux pumps is an attractive response to the emergence of MDR phenotypes in infectious bacteria. In the present study, various alkylaminoquinolines were tested as potential inhibitors of drug transporters. We showed that alkylaminoquinolines are capable of restoring susceptibilities to structurally unrelated antibiotics in clinical isolates of MDR Gram-negative bacteria. Antibiotic efflux studies indicated that 7-nitro-8-methyl-4-[2´-(piperidino)ethyl]aminoquinoline acts as an inhibitor of the AcrAB–TolC efflux pump and restores a high level of intracellular drug concentration. Inhibitory activity of this alkylaminoquinoline is observed on clinical isolates showing different resistance phenotypes.

scholarly journals Analysis of Helicobacter pylori’s Antibiotic Resistance Rate and Research on Its Eradication Treatment Plan

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Li Jiao ◽  
Junmin Wang ◽  
Huan Ma
Keyword(s):  
Drug Resistance ◽  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Treatment Plan ◽  
Drug Susceptibility ◽  
Resistance Rate ◽  
Clinical Isolates ◽  
Treatment Plans ◽  
H Pylori ◽  
The Right

How to choose the right plan is the key to treatment, and this must take into account the local eradication of Helicobacter pylori and the drug resistance of Helicobacter pylori. In order to better eradicate Helicobacter pylori, in the current clinical treatment process, most of the combined treatments of triple drugs are used, but the therapeutic effect is still not ideal. In addition, many studies have focused on changing the types and dosages of drugs, but they have not yet achieved good results. This paper combines experimental research to analyze the drug resistance rate of Helicobacter pylori and obtains gastric mucosal specimens of patients through gastroscopy to cultivate clinical isolates of H. pylori.. Furthermore, this study used the Kirby-Bauer drug susceptibility disc technique to determine the sensitivity of H. pylori clinical isolates to a range of regularly used clinical antibiotics, as well as a set of instances of H. pylori antibiotic resistance. Finally, this research integrates experimental analyses and various successful eradication treatment plans to provide a unique eradication treatment strategy.

scholarly journals Resistance to Azithromycin and β-Lactam Antibiotics by Clinical Isolates of E. coli Isolated from Dhaka, Bangladesh

2019 ◽  
Vol 34 (2) ◽  
pp. 61-66
Author(s):  
Sunjukta Ahsan ◽  
Mayen Uddin ◽  
Juthika Mandal ◽  
Marufa Zerin Akhter
Keyword(s):  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Resistance Patterns ◽  
Ready Availability ◽  
Antibiotic Resistance Patterns

Antibiotic resistant E. coli are prevalent in Bangladesh. The indiscriminate use of antimicrobials and ready availability of over the counter drugs are responsible for this. This study was conducted to investigate the susceptibility of clinical Escherichia coli to the antibiotics Imipenem, Ceftriaxone, Ceftazidime and Azithromycin. Kirby-Bauer disk diffusion method was used to determine sensitivity to antimicrobials. Agar based assay was employed for the detection of efflux pumps. PCR was used amplify antibiotic resistance genes.All isolates were resistant to Ceftriaxone whereas most were sensitive to Imipenem. The MICs of Ceftazidime and Azithromycin ranged between 128 μg/ml and 256 μg/ml. The prevalence of ²-lactamase producers was 57.89 % with 36.84 % of the isolates exhibiting ESBL activity. No specific correlation could be found between plasmid sizes and antibiotic resistance patterns. Efflux pump was found to be involved in Azithromycin resistance in 63.15% of the isolates. The gene for phosphotransferase, mph(A) was the most common among the macrolide modifying genes, being present in 73.68% (14/19) of the isolates followed by both erm(A) anderm(C) esterases each present in 10.53% (2/19) isolates. This study concluded that clinical isolates of E. coli in Bangladesh could be resistant to multiple classes of antibiotics through different mechanisms of resistance. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 61-66

scholarly journals Unraveling the Novel Effect of Patchouli Alcohol Against the Antibiotic Resistance of Helicobacter pylori

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanzun Zhong ◽  
Liyao Tang ◽  
Qiuhua Deng ◽  
Li Jing ◽  
Jiao Zhang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Underlying Mechanism ◽  
Gastrointestinal Diseases ◽  
Bactericidal Effect ◽  
Patchouli Alcohol ◽  
H Pylori

The long-term colonization of Helicobacter pylori can cause various gastrointestinal diseases, and its high genetic variability is prone to antibiotic resistance and leads to failure of clinical treatment. Intracellular survival also contributes to the drug tolerance of H. pylori. Patchouli alcohol (PA) shows a highly efficient activity against H. pylori in vitro and in vivo. And this study aims to explore whether PA can reduce the resistance of H. pylori and determine the underlying mechanism. Checkerboard and time–kill bactericidal curve assay reveal that the combination of PA and clarithromycin (CLR) promoted the inhibition and bactericidal effect against H. pylori. Stimulation of CLR leads to the internalization of H. pylori, but PA can effectively inhibit the invasion induced by CLR. Compared with antibiotics, PA remarkably eradicated the intracellular H. pylori, and this intracellular sterilized ability was further improved in combination with antibiotics (CLR and metronidazole). The expression of H. pylori efflux pump genes (hp0605, hp1327, and hp1489) was dose-dependently downregulated by PA. Digital droplet PCR indicated that the H. pylori mutant of A2143G can be inhibited by PA. Cellular uptake and transport assays showed that PA is rapidly absorbed, which promotes its activity against intracellular bacteria. Therefore, PA can act synergistically with CLR as a candidate treatment against drug-resistant H. pylori.

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