Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Solanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.

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Plant Regeneration of Withania somnifera (L.) Dunal (Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant in Bangladesh

Journal of Biological Sciences ◽

10.3923/jbs.2004.219.223 ◽

2004 ◽

Vol 4 (2) ◽

pp. 219-223 ◽

Cited By ~ 11

Author(s):

N.A. Siddique . ◽

M.A. Bari . ◽

Sharmin Shahnewaz . ◽

M.H. Rahman . ◽

M.R. Hasan . ◽

...

Keyword(s):

Plant Regeneration ◽

Medicinal Plant ◽

Withania Somnifera ◽

Nodal Segments ◽

Endangered Medicinal Plant

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PROTOCORM-LIKE BODY FORMATION AND PLANT REGENERATION OF DENDROBIUM HUOSHANENSE, AN ENDANGERED MEDICINAL PLANT

Acta Horticulturae ◽

10.17660/actahortic.2006.725.49 ◽

2006 ◽

pp. 379-384 ◽

Cited By ~ 4

Author(s):

Y. Wang ◽

J.P. Luo ◽

X.Q. Zha

Keyword(s):

Plant Regeneration ◽

Medicinal Plant ◽

Body Formation ◽

Endangered Medicinal Plant ◽

Dendrobium Huoshanense

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High-frequency plant regeneration and histological analysis of callus in Cichorium intybus: An important medicinal plant

Journal of Phytology ◽

10.19071/jp.2016.v8.2980 ◽

2016 ◽

Vol 8 (1) ◽

Cited By ~ 2

Author(s):

K. Dakshayini ◽

C. Vaman Rao ◽

Anitha Karun ◽

U. Bhavyashree ◽

P. Ujwal

Keyword(s):

Acetic Acid ◽

Plant Regeneration ◽

Medicinal Plant ◽

High Frequency ◽

Leaf Disc ◽

Leaf Explants ◽

Cichorium Intybus ◽

Naphthalene Acetic Acid ◽

Regenerated Plants

An efficient in vitro propagation and in vitro flowering protocols were developed for the medicinal plant Cichorium intybus (Asteraceae) using leaf disc explants. Media supplemented with the growth regulator naphthalene acetic acid (NAA) (1.5 mg/l) + 6-benzyle adenine (0.25 mg/l) was used for the initial induction of the callus and further subcultured to the same media for the proliferation of the callus. Pale yellow and green calli were noticed, which depends on incorporation of the growth hormones and their varying concentrations. Murashige and Skoog medium in addition with 2 mg/l kinetin+ 0.5 mg/l indole-3-acetic acid (IBA) + 500 mg/l casein hydrolysate resulted in maximum regeneration. Media supplemented via IBA (0.5 mg/l) and NAA (0.5 mg/l) (98%) was found to be optimum for rhizogenesis for in vitro regenerated plants. For acclimatization 5-6 weeks mature in vitro regenerated plants were transferred into the greenhouse for acclimatization. The histological study revealed the presence actively dividing meristematic cells in callus. The occurrence of the peripheral meristematic zone associated with callus was noticed in after 20 days, which formed the shoot meristems after 45 days of incubation. To our knowledge, this is the first report on high-frequency plant regeneration which was carried out indirectly from the leaf explants which was grown in controlled environment with varying concentration of the growth regulators and histology of callus of different stages from leaf explants of C. intybus.

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