scholarly journals Phylogenetic relationships among different morphotypes of StY-genomic species Elymus ciliaris and E. amurensis (Poaceae) as a unified macroevolutional complex

2021 ◽  
Author(s):  
Alexander V. Agafonov ◽  
◽  
Elena V. Shabanova ◽  
Maria V. Emtseva ◽  
Sergei V. Asbaganov ◽  
...  
Keyword(s):  
Early Treatment ◽  
Morphological Traits ◽  
Nuclear Gene ◽  
Experimental Methods ◽  
Component Composition ◽  
Issr Markers ◽  
Neighbor Joining ◽  
Genomic Species ◽  
Protein Spectra ◽  
Far Eastern

Microevolutionary relationships between the Far Eastern StY-genomic species Elymus ciliaris and E. amurensis were studied using a set of experimental methods. No relationship was found between the formal species affiliation of a particular accession and the component composition of protein spectra. The consensus neighbor-joining (NJ) dendrogram based on the variability of ISSR markers showed the features of differentiation among different morphotypes of the two species. Pubescence of leaf blades is not a marker of differences between the studied species. A level of crossability in E. ciliaris s. l. did not allow to study the inheritance of morphological traits. According to the results of sequencing of the nuclear gene GBSS1, the sequences of the St subgenome are the most informative in terms of microevolutionary differentiation. It is proposed to return to the early treatment by N.N. Tsvelev, where E. ciliaris and E. amurensis were considered as subspecies of E. ciliaris s. l.

scholarly journals Genetic Signature of a Past Anthropogenic Transportation of a Far-Eastern Endemic Cladoceran (Crustacea: Daphniidae) to the Volga Basin

Water ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 2589
Author(s):  
Dmitry P. Karabanov ◽  
Petr G. Garibian ◽  
Eugeniya I. Bekker ◽  
Rimma Z. Sabitova ◽  
Alexey A. Kotov
Keyword(s):  
Species Complex ◽  
Morphological Traits ◽  
Far East ◽  
Volga Basin ◽  
28S Rrna ◽  
Water Flea ◽  
The Far East ◽  
Nuclear Loci ◽  
Cryptic Invasions ◽  
Far Eastern

Most studies of water flea (Crustacea: Cladocera) invasions are concentrated on a few taxa with an obvious harmful influence on native ecosystems, while our knowledge of cases of anthropogenic introduction with not-so-obvious consequences, in most other taxa, is poor. We found in the Volga basin (European Russia) a population that contained D. curvirostris Eylmann, 1887 and its hybrids with D. korovchinskyi Kotov et al. 2021. The latter taxon is endemic to the Far East and it has appeared in the Volga basin as a result of past human-mediated transportation. The population from Bakhilovo is represented by two strongly different groups of the COI haplotypes belonging, respectively, to (1) D. curvirostris and (2) D. korovchinskyi. We detected SNPs in the position 60 of the HSP-90ex3 locus and in the 195 positions of 28S rRNA locus, which differentiate two species. Part of the specimens from Bakhilovo belonged to D. curvirostris s.str., demonstrating homozygote SNP sites in two loci, but two specimens had heterozygote SNP sites in both nuclear loci. They belong to D. curvirostris x korovchinskyi hybrids. Most morphological traits of the females were characteristic of D. curvirostris. We found in some specimens some characters which could suggest their hybrid status, but this opinion is a hypothesis only, which needs to be checked on more ample material. The exact hybrid system in this pond is not known. Moreover, we have no evidences of sexual reproduction of the hybrids; they could reproduce by parthenogenesis only as is known for hybrids of the D. pulex group, or continuously crossing with parents like some members of D. longispina group. However, poor parental D. korovchinskyi was not detected in the pond either morphologically or genetically. The exact vector of its past anthropogenic transportation to the Volga is unknown. Most probably, just ephippia of D. korovchinskyi were translocated replaced from the Khabarovsk Territory to the Samara Area somehow. This is the first report on hybrids within the D. curvirostris species complex. Here, we demonstrated that accurate studies with deep resolution increase the number of revealed cryptic invasions. We expect that the number of revealed cases of cryptic interspecific invasions will grow rapidly.

scholarly journals Assessment of relationships among and within Helichrysum Mill. (Asteraceae) species by using ISSR markers and morphological traits

Hacquetia ◽  
2019 ◽  
Vol 18 (1) ◽  
pp. 105-118 ◽  
Author(s):  
Narjes Azizi ◽  
Masoud Sheidai ◽  
Valiollah Mozaffarian ◽  
Mitra Arman ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Similarity ◽  
Morphological Traits ◽  
Morphological Analysis ◽  
Gene Diversity ◽  
Molecular Data ◽  
Issr Markers ◽  
Diagnostic Features ◽  
Analysis Of Molecular Variance ◽  
Information Index ◽  
Expected Heterozygosity

Abstract This study conducted to determine relationship among and within Iranian Helichrysum species (Asteraceae). In this study based on ISSR markers, the highest percentage of ISSR loci polymorphism (54.7%) occurred in H. armenium. The highest gene diversity over loci (1.224), Shannon’s Information Index (0.224%) and Expected Heterozygosity (0.142%) occurred in H. armenium (0.18) and the lowest of these parameters (0%) were observed in H. araxinum, H. graveolens, H. persicum and H. psychrophilum. The highest genetic similarity occurred between H. armenium and H. rubicundum (0.989), while the lowest was between H. polyphyllum and H. graveolens (0.213). The analysis of molecular variance (AMOVA), showed significant genetic variation among (24%) and within (76%) species. In morphological analysis traits such as indumentum, resting bud, achene length, achenial papillae, dimension of receptacle and form and apex of phyllaries were main diagnostic features. Results obtained from the morphological cluster were greatly consistent with the molecular data, to elucidating taxonomic relationships, as well as both attributed the higher diversity in H. armenium and H. rubicundum in comparison with other species and also indicated that H. persicum is a member of H. oocephalum species. Totally we confirmed the presence of 18 species in Iran.

Genetic evidence supports Sylvilagus mansuetus (Lagomorpha: Leporidae) as a subspecies of S. bachmani

Zootaxa ◽  
2016 ◽  
Vol 4196 (2) ◽  
pp. 289
Author(s):  
SERGIO TICUL ÁLVAREZ-CASTAÑEDA ◽  
CONSUELO LORENZO
Keyword(s):  
Phylogenetic Relationships ◽  
Morphological Traits ◽  
Nuclear Gene ◽  
Sister Group ◽  
Genetic Distances ◽  
Mitochondrial Genes ◽  
The Other ◽  
Cyt B ◽  
San Jose ◽  
Allopatric Distribution

Since Sylvilagus bachmani (Lagomorpha: Leporidae) from the Baja California Peninsula and S. mansuetus from San Jose Island, Mexico, display an allopatric distribution and are closely related, their taxonomy is unclear. The phylogenetic relationships among specimens of both species were evaluated using two mitochondrial genes (Cyt b, COI) and the beta-fibrinogen nuclear gene intron 7 (β-fib I7). The genetic analyses revealed that S. mansuetus was included within the S. bachmani clade as the sister-group of S. b. cerrosensis. The genetic distances among S. b. cerrosensis and mansuetus were relatively low (1.3% with Cyt b), similar to intraspecific distances observed within other species of Sylvilagus. We consider mansuetus to be a subspecies of S. bachmani, and the morphological traits previously used to differentiate the two taxa should be used to distinguish S. b. mansuetus from the other subspecies of S. bachmani. 

Assessment of the genetic diversity and relationships of a collection of Mentha spp. in Tunisia using morphological traits and ISSR markers

2019 ◽  
Vol 95 (4) ◽  
pp. 483-495
Author(s):  
Z. Soilhi ◽  
H. Trindade ◽  
S. Vicente ◽  
S. Gouiaa ◽  
H. Khoudi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Morphological Traits ◽  
Issr Markers

scholarly journals GENETIC DIVERSITY OF TULIPA SUAVEOLENS ROTH POPULATIONS IN VOLGOGRAD PROVINCE

2020 ◽  
Vol 180 (4) ◽  
pp. 88-93
Author(s):  
T. A. Kritskaya ◽  
A. S. Kashin
Keyword(s):  
Natural Habitat ◽  
Molecular Genetic ◽  
Issr Markers ◽  
Genetic Group ◽  
Conservation Strategy ◽  
Neighbor Joining ◽  
Issr Analysis ◽  
Species Preservation ◽  
Natural Parks ◽  
Protected Natural Areas

Background. The current natural habitat of Tulipa suaveolens Roth rapidly decreases. In order to work out the strategy of the species’ preservation, evaluation of its intraand interpopulation polymorphism is required.Materials and methods. Molecular-genetic ISSR markers were used to analyze 125 samples from 10 populations of T. suaveolens occurring in Volgograd Province and 4 populations from Saratov Province.Results. ISSR analysis revealed high intrapopulation polymorphism (73–89%) in T. suaveolens populations form Volgograd Province. AMOVA attributed the largest proportion of variability (74.3%) to intrapopulation polymorphism. Interpopulation differences account for 25.7%. Total subdivision of populations (FST) was 0.257; total gene flow (Nm) between populations was 0.723. According to Bayesian analysis and clustering with both UPGMA and Neighbor Joining methods, all the studied T. suaveolens samples from Volgograd Province make up a large genetic group: within that group none of the potential subgroups may be associated with a particular place of collecting. The NewHybrids software was applied, and the results pointed to the hybrid nature of most samples. Samples of three populations from Saratov Province made up a separate genetic group; those samples fell under the category of parent forms.Conclusion. Considering that genetic subdivision of T. suaveolens populations within the administrative borders of Volgograd Province is insignificant, while all the province’s natural parks and a number of protected natural areas undertake measures to preserve the species, the existing conservation strategy may be recognized as effective and sufficient.

scholarly journals Genetic diversity assessment among Corema album (L.) D. Don (Ericaceae) genotypes based on ISSR markers and agro-morphological traits

2019 ◽  
Vol 67 (3) ◽  
pp. 715-726
Author(s):  
João Jacinto ◽  
Pedro Brás de Oliveira ◽  
Teresa Valdiviesso ◽  
Jorge Capelo ◽  
Pedro Arsénio ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Morphological Traits ◽  
Issr Markers ◽  
Diversity Assessment

scholarly journals Genetic diversity revealed by morphological traits and ISSR markers in 48 Okras (Abelmoschus escullentus L.)

2015 ◽  
Vol 21 (3) ◽  
pp. 359-364 ◽  
Author(s):  
Cong-Ying Yuan ◽  
Ping Wang ◽  
Pang-Pang Chen ◽  
Wen-Jun Xiao ◽  
Cheng Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Morphological Traits ◽  
Issr Markers

scholarly journals Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers

2020 ◽  
Vol 50 (3) ◽  
pp. 204-212
Author(s):  
Stalin Juan Vasquez GUIZADO ◽  
Muhammad Azhar NADEEM ◽  
Fawad ALI ◽  
Muzaffer BARUT ◽  
Ephrem HABYARIMANA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Diversity ◽  
Population Level ◽  
Diversity Indices ◽  
Issr Markers ◽  
Neighbor Joining ◽  
Peruvian Amazon ◽  
Over Exploitation ◽  
Issr Primers

ABSTRACT Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.

scholarly journals POLYMORPHISM IN LIPPIA ALBA CLONES FROM THE METROPOLITAN REGION OF RIO DE JANEIRO

2017 ◽  
Vol 7 (2) ◽  
pp. 1044-1049
Author(s):  
Uira do Amaral ◽  
Mauricio Ballesteiro Pereira ◽  
Pedro Correa Damasceno Junior ◽  
Marco Andre Alves de Souza ◽  
Miklos Maximiliano Bajay
Keyword(s):  
Genetic Variability ◽  
Rio De Janeiro ◽  
Issr Markers ◽  
Geographic Region ◽  
Metropolitan Region ◽  
Natural Occurrence ◽  
Neighbor Joining ◽  
Lippia Alba ◽  
Polymorphic Bands ◽  
Issr Primers

In Brazil, the natural occurrence of the species Lippia alba (Mill) N. E. Brown is broad and diffuse, which justifies a high phenotypic variation and the occurrence of several chemotypes. Furthermore, the molecular methods help to determine the genetic variability with greater precision, in relation to the morphological methods. Thus, the objective of this work was to evaluate the genetic variability of different genotypes of L. alba from the Metropolitan region of the State of Rio de Janeiro using ISSR markers. The experiment was conducted at the Institute of Agronomy of the UFRRJ, from twenty genotypes of L. alba. Thirteen ISSR primers were used, obtaining 97 amplified bands and 74 polymorphic bands, equivalent to 77.89% of the amplified bands. The dendrogram (Neighbor Joining) formed five groups and just the genotypes UFRRJ LA01 and UFRRJ LA02 were grouped by the same geographic region and chemotype. The lowest similarity was observed in the grouping that joined the accessions UFRRJ LA14 and UFRRJ LA06. These results confirm the genetic variation between the genotypes, even when originating from the same region. L. alba genotypes from the Metropolitan Region of Rio de Janeiro show genetic and phytochemical variability.

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