Assessing ursolic acid contents of some commonly consumed herbs grown in Turkey

Extracts of Carolina jasmine (Gelsemium sempervirens (L.) J.St.-Hil.) petals were evaluated in vitro for inhibition activity against protein tyrosine phosphatase 1B (PTP1B). The principle active agent was also isolated from the extract and identified as ursolic acid (1). This is the first report of ursolic acid from G. sempervirens and of PTP1B-inhibiting activity in the genus Gelsemium.

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Synergism Effects of Ursolic Acid Supplementation on the Levels of Irisin, C-reactive Protein, IL-6, and TNF-α During High-intensity Resistance Training in Low Activity Men

Cardiovascular & Haematological Disorders - Drug Targets ◽

10.2174/1871529x19666190918144727 ◽

2020 ◽

Vol 20 (2) ◽

pp. 138-144 ◽

Cited By ~ 1

Author(s):

Ehsan Asghari ◽

Amir Rashidlamir ◽

Seyyed R.A. Hosseini ◽

Mahtab Moazzami ◽

Saeed Samarghandian ◽

...

Keyword(s):

Resistance Training ◽

Ursolic Acid ◽

High Intensity ◽

Plasma Levels ◽

Group Versus ◽

Current Data ◽

C Reactive Protein ◽

Reactive Protein ◽

Tnf Α ◽

Low Activity

Background:: Ursolic Acid (UA) is a pentacyclic triterpenoid carboxylic acid which is extracted from plants. UA may enhance the effect of Resistance Training (RT) in human. Objective: Current research was designed to show the effect of High-Intensity Resistance Training (HIRT) in the presence or absence of UA on the serum levels of irisin, CRP, IL-6 and TNF-α in the low activity men. Method:: The study included twenty-two healthy male HIRT with placebo, supplementation, and HIRT in the presence of UA supplementation. The two groups received eight-week intervention including 2 sets of 8 exercises, with 8~10 repetitions at 70~75% of 1 repetition maximum and a 2 min rest interval between sets, performed 3 times/week. Placebo or UA orally was evaluated as 1 capsule 3 times/day during 8 weeks. The subsequent factors were measured post- and preintervention: C-Reactive Protein (CRP), Irisin, Tumor Necrotic Factor (TNF-α) and Interleukin-6 (IL-6). Results:: UA supplementation significantly increased the plasma levels of irisin in the HIRT+UA group versus the HIRT+P group (p<0.05). UA treatment also dramatically decreased the plasma levels of CRP, IL-6, and TNF-α in the HIRT+UA group versus the HIRT+P group (p<0.05). Conclusion:: The current data showed that UA-induced an increase in serum irisin and reduction of CRP, IL-6, and TNF-α may have beneficial effects as a chemical for increasing of the effects of HIRT in low activity men.

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Ursolic Acid and Oleanolic Acid: Pentacyclic Terpenoids with Promising Anti-Inflammatory Activities

Recent Patents on Inflammation & Allergy Drug Discovery ◽

10.2174/1872213x10666160711143904 ◽

2016 ◽

Vol 10 (1) ◽

pp. 21-33 ◽

Cited By ~ 50

Author(s):

Dharambir Kashyap ◽

Ajay Sharma ◽

Hardeep S. Tuli ◽

Sandeep Punia ◽

Anil K. Sharma

Keyword(s):

Ursolic Acid ◽

Oleanolic Acid ◽

Anti Inflammatory

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Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid

Current Traditional Medicine ◽

10.2174/2215083805666191010152439 ◽

2020 ◽

Vol 6 (2) ◽

pp. 134-146 ◽

Cited By ~ 3

Author(s):

Kehkashan Arshad Qamar ◽

Ahsana Dar Farooq ◽

Bina S. Siddiqui ◽

Nurul Kabir ◽

Sabira Begum

Keyword(s):

Ursolic Acid ◽

Oleanolic Acid ◽

Mitotic Spindle ◽

Immunofluorescence Microscopy ◽

Methanolic Extract ◽

Folk Medicine ◽

Ocimum Basilicum ◽

Antiproliferative Effects ◽

Ancient Times ◽

Mcf 7

Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Objective: To emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy. Method: O. basilicum (aerial parts) methanolic extract and fractions were screened against HT-144, MCF-7, NCI-H460 and SF-268 human cancer cell lines using sulforhodamine B assay. The more active Petroleum Ether Insoluble (PEI) fraction was fractionated into six sub-fractions (OB-1 to OB-6). Four pure compounds (3-O-methyl ursolic acid, oleanolic acid, 3-epi-ursolic acid and ursolic acid) were isolated from the more potent sub-fraction OB- 6. Triple channel immunofluorescence microscopy was employed to observe the effects of methanolic extract, PEI fraction, sub-fractions OB-5 and OB-6, 3-epi-ursolic acid and oleanolic acid on the cytoskeleton and nuclei of MCF-7 cells. Result: The methanolic extract and the PEI fraction exhibited selectively greater growth inhibition against MCF-7 cell line (TGI: 56 and 36.2 µg/ml, respectively). By using triple channel immunofluorescence microscopy, it was observed that the methanolic extract, PEI fraction, sub-fraction OB-5 and 3-epi-ursolic acid induced irregular mitotic spindle formation and slowing of mitotic progression in MCF-7 cells while sub-fraction OB-6 induced mitotic arrest in the prophase stage. F-actin aggregation was also visible in PEI fraction, subfraction OB-5 and 3-epi-ursolic acid treated MCF-7 cells. Conclusion: These results emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy and suggest that it inhibits the growth of MCF-7 cancer cells via multiple mechanisms such as interaction with the microtubules and mitotic spindle apparatus, and F-actin aggregation.

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