scholarly journals Absence of natural group anti-A, anti-B antibodies (isohemagglutinins) and blood transfusion tactics in this blood group

2015 ◽  
Vol 96 (3) ◽  
pp. 425-428
Author(s):  
R G Turaev ◽  
E E Bel’skaya ◽  
N S Somova ◽  
M A Egorova
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Serum Analysis ◽  
Blood Components ◽  
Clinical Diagnostic Laboratory ◽  
Diagnostic Laboratory ◽  
Laboratory Methods ◽  
Natural Group ◽  
Plasma Coagulation ◽  
Group Serum

Aim. To analyze the clinical data obtained at donor and recipient blood testing obtained in clinical diagnostic laboratory. Methods. Blood of donors and recipients for erythrocyte-containing blood-derived products was examined. Gel method was used for immunologic and hematologic testing. Results. A donor and recipients with the absence of natural group antibodies (isohemagglutinins) were identified. Those subjects were consulted for treatment with blood transfusion. According to the regulations, blood transfusion is provided considering the blood group of recipient. In described cases, serum analysis revealed no natural group antibodies, hence, no antibodies should be present in administered plasma coagulation hemostasis correctors. Conclusion. In the presented clinical cases, due to the absence of natural group antibodies (isohemagglutinins) АВ0 (IV) group serum is preferable if the blood transfusion is needed. Keywords: blood components, natural group antibodies, isohemagglutinins, agammaglobulinemia, Wiskott-Aldrich syndrome, non-Hodgkin’s lymphoma.

scholarly journals The rationale for the post-transfusion reactions prevention program in rh-positive patients

2013 ◽  
Vol 94 (5) ◽  
pp. 764-765
Author(s):  
R G Turaev ◽  
E E Bel’skaya
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Prevention Program ◽  
Blood Components ◽  
Negative Consequences ◽  
Antigen Profile ◽  
Transfusion Reactions ◽  
Antibodies Detection

A case of a patient with А 2В (IV) Rh 0(D) + blood group, CcDEeK - phenotype, in whom anti-erythrocyte antibodies (+) were found and specified as anti-D antibodies, is presented. The Rh compatibility tests were positive with 28% Rh 0(D) +-donors (incompatibility) and negative with all Rh 0(D) --donors (compatibility) as well as with own erythrocytes (compatibility). The case indicates that a timely extensive examination of a patient’s blood (antigen profile examination with anti-erythrocyte antibodies detection) before blood transfusion guarantees the blood transfusion safety and reduces the rate of negative consequences of erythrocyte-containing blood components, including hemolytic reactions.

scholarly journals The Features of Antigen Prevalence of Rhesus System in Donor Population

2021 ◽  
Vol 15 ◽  
pp. 76-86
Author(s):  
M. Nagervadze ◽  
L. Akvlediani ◽  
I. Tsintsadze ◽  
T. Koiava ◽  
R. Loria ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Blood Cells ◽  
Blood Donors ◽  
Single Case ◽  
Health Centre ◽  
Blood Components ◽  
Negative Group ◽  
Diagnostic Laboratory ◽  
Donor Population ◽  
D Antigen

Research materials and methods. 852 voluntary Georgian blood donors have been typed on red blood cells group antigens. The research materials have taken from the diagnostic laboratory of Health Centre of Batumi (Georgia republic). The immunoserological methods with monoclonal anti –AB, -B, -A, A1, -A2 (H), -C, -c, - D, -E, -e (Bio-Rad, cypress diagnostics) antibodies was used for typing blood. The ID cards, such as ABO/D + Reverse Grouping (Bio-Rad) were also used for typing of erythrocyte antigens. Result. Prevalence of Rh system antigens in the studied group is looks like so: e antigens – 94,6%, c antigens -85%, C-68,03, E antigens - 38,07%. The majority (84%) of the studied donors are Rh-positive (n=719), 133 (16%) donors are Rh-negative. C antigen most common is present in the combination with D antigen. 65, 8 % case donors had CD+ combination (n=561). E antigen in most cases is presented with a combination of D antigen. 36, 9% of the studied donors (n=306) had ED+ combination. A miserable number of studied donors had CD - (2,23%; n=19) and ED - (1,17%; n=9) combinations. We have studied the Rh phenotypes prevalence in blood donors. According to RHD, RHC, and RHE gene loci, there are 18 theoretically possible phenotypical groups. Among them half (nine) are Rh-positive and the rest of them are Rh-negative. The Rh-positive phenotypes are: CDE; CDEe; CDe; CcDE; CcDEe; CcDe; ccDE; cDEe and cDe. Rh-negative phenotypes are CdE; CdEe; Cde; CcdE; CcdEe; Ccde; cdE; cdEe; cde. We allocated 17 Rh phenotypes among studied donors. Only one phenotype CdE, which belongs to Rh negative group, was not present in studied donors. Other 17 phenotypes showed different frequencies. Some of them were only in a single case, for example, cdEe, cdE, CdEe phenotypes had only one donor. The majority of the phenotype in he studied donors (27,8±1,53%) was CcDe (n=237). CcDEe -19,3±1,35% (n=165); 125 donors have CDe phenotype (14,6±1,2); The frequency of cde was 13,1±1,5%, which means that 112 studied donors belonged to this phenotype group; 87 studied donors had cDEe phenotype characteristics (10,2%); The frequency of cDe was 4,9% (n=42); 19 donors had CDEe phenotype. Other phenotypes (CDE, Cde, CcdEe, Ccde) frequency was very low. Conclusion. Our studied donors are characterized by rather high polymorphism. The Georgian donor’s population is heterogenic, especially high heterogeneity are shown in Rh positive phenotypes. The obtained data is vital importance for the preparation of whole blood or certain blood components for the purpose of their rational usage in blood transfusion.

scholarly journals Preemptive intravenous iron therapy versus autologous whole blood therapy for early postoperative hemoglobin level in patients undergoing bimaxillary orthognathic surgery: a prospective randomized noninferiority trial

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Min Suk Chae ◽  
Mihyun Lee ◽  
Min Ho Choi ◽  
Je Uk Park ◽  
Misun Park ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Whole Blood ◽  
Orthognathic Surgery ◽  
Hemoglobin Level ◽  
Iron Therapy ◽  
Iron Group ◽  
Autologous Whole Blood ◽  
Bimaxillary Orthognathic Surgery ◽  
Iv Iron

Abstract Background Previous studies have reported the efficacy and safety of intravenous (IV) iron therapy during the perioperative period as an alternative and adjunct to allogeneic blood transfusion. Preemptive IV iron therapy provides noninferior hemoglobin levels on postoperative day (POD) 1 compared to autologous whole blood therapy (AWBT) in healthy patients who had undergone bimaxillary orthognathic surgery. Methods This was a prospective, patient-randomized, noninferiority trial. After excluding 2 patients, 64 patients were divided into two groups: the IV iron therapy group (patients received IV iron infusion 4 weeks before surgery; n = 32) and the AWBT group (2 units of autologous whole blood were collected 4 and 2 weeks before surgery; n = 32). The primary outcome was hemoglobin level on POD 1 and the prespecified noninferiority limit was − 1 g/dL. Results Baseline data were comparable, including hemoglobin and iron levels, between the two groups. Immediately before surgery, the levels of hemoglobin, iron, and ferritin were higher in the IV iron group than in the AWBT group. The mean treatment difference (iron group—whole blood group) in hemoglobin level on POD 1 between the two groups was 0.09 (95% CI = − 0.83 to 1.0). As the lower limit of the 95% CI (− 0.83) was higher than the prespecified noninferiority margin (δ = − 1), noninferiority was established. On POD 2, the hemoglobin level became lower in the iron group, which eventually led to greater requirement of allogeneic blood transfusion compared to the whole blood group. However, the iron group did not require allogeneic blood transfusion during or early after surgery, and the whole blood group showed continuously higher incidence of overt iron deficiency compared to the iron group. Conclusion As collection of autologous whole blood caused overt iron loss and anemia before surgery and intraoperative transfusion of whole blood was not able to prevent the occurrence of persistent iron deficiency after surgery, IV iron therapy was found to have potential benefits for iron homeostasis and subsequent erythropoiesis in healthy patients early after bimaxillary orthognathic surgery. Trial registration: Clinical Research Information Service, Republic of Korea, approval number: KCT0003680 on March 27, 2019. https://cris.nih.go.kr/cris/search/search_result_st01_kren.jsp?seq=15769&sLeft=2&ltype=my&rtype=my.

scholarly journals Analysis of blood transfusion request and utilization pattern at teaching hospital in Eastern Nepal

2018 ◽  
Vol 8 (2) ◽  
pp. 1374-1377 ◽  
Author(s):  
Santosh Upadhyaya Kafle ◽  
Krishna Kumar Jha ◽  
Mrinalini Singh ◽  
Shilpi Shaukin
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Teaching Hospital ◽  
Whole Blood ◽  
Age Group ◽  
Utilization Pattern ◽  
Total Blood ◽  
Cross Sectional ◽  
Male And Female ◽  
Gynecology And Obstetrics

Background: Blood transfusion unit collection, storage and dispatching on Birat Medical College and Teaching Hospital began from 19 June 2016 via blood bank. After which, many blood distribution were made accessible via this service. This study analyzes the blood transfusion request and utilization pattern and also to monitor for managing the bloodstocks meeting present and future demand in hospital. Materials and Methods: This is descriptive cross-sectional study conducted for all the requests of whole blood dispatched from 19th June, 2016 to 04th July, 2018. Whole blood requisitions from various departments were reviewed regarding the number of requisition, total blood unit replaced, blood group and socio-demographic profile of patients.Results: Of 648 transfusion units dispatched, 131 and 324 patients were male and female respectively, B positive blood group were most common, followed by A positive, O positive, AB positive, A negative, O negative and AB negative respectively. The largest numbers of requisitions were from Gynecology and Obstetric department in the hospital of 258 requisitions followed by internal medicine, surgery department, postoperative ward and orthopaedic department. Majority of the requisitions were for 20-29 years age group from the local district Morang. The total blood units replaced were 75, of which 29 and 46 of male and female respectively. Conclusions: B Positive was the predominant requested blood group with Gynecology and Obstetrics department making the most requests, among 20-29 years age group from Morang district. The total replaced blood units were less in number with some requisition forms even lacked essential details.

Improved HPLC Determination of Plasma and Urine Oxalate in the Clinical Diagnostic Laboratory

1992 ◽  
Vol 15 (3) ◽  
pp. 501-511 ◽  
Author(s):  
A. Brega ◽  
A. Quadri ◽  
P. Villa ◽  
P. Prandini ◽  
Ji-Qing Wei ◽  
...  
Keyword(s):  
Hplc Determination ◽  
Clinical Diagnostic Laboratory ◽  
Diagnostic Laboratory ◽  
Clinical Diagnostic ◽  
Urine Oxalate

Organization of the work of the medical laboratory service in accordance with the requirements of the standard GOST ISO 15189–2015 «Medical laboratories. Particular requirements for quality and competence»

2020 ◽  
pp. 15-22
Author(s):  
Elena Vitalievna Perminova
Keyword(s):  
Clinical Laboratory ◽  
Medical Laboratory ◽  
Laboratory Research ◽  
Laboratory Diagnostics ◽  
Clinical Diagnostic Laboratory ◽  
Diagnostic Laboratory ◽  
Iso 15189 ◽  
Laboratory Service ◽  
Medical Laboratories

Clinical laboratory diagnostics is a medical specialty, which is based on in vitro diagnostic studies of biomaterial obtained from an individual. At the present stage, there are three main types of organization of the laboratory research process — a laboratory service as part of a medical and preventive institution, a centralized laboratory where biomaterials are delivered for research from various healthcare institutions, as well as mobile laboratories that allow conducting the research directly at the patient’s bedside. This discipline involves the use of a wide variety of diagnostic research methods and the use of a huge number of specific techniques. Their list should include carrying out hematological, microbiological, virological, immunological, serological, parasitic, and biochemical studies. Also, when organizing laboratory diagnostic activities, a number of other studies (cytological, histological, toxicological, genetic, molecular biological, etc.) are provided. A laboratory report is formulated after obtaining clinical data and comparing them with the obtained test results. The quality of laboratory tests is ensured through the systematic implementation of internal laboratory control, as well as participation in a national program for external quality assessment. The activities of the clinical diagnostic laboratory should be organized in accordance with the requirements of the standard GOST R ISO 15189–2015 «Medical laboratories. Particular requirements for quality and competence», which is based on the provisions of two more fundamental standards — ISO 9001 and ISO 17025, and adds a number of special requirements related to medical laboratories.

scholarly journals Improved Detection of Staphylococcus intermedius Group in a Routine Diagnostic Laboratory

2014 ◽  
Vol 53 (3) ◽  
pp. 961-963 ◽  
Author(s):  
John Lee ◽  
Aimee Murray ◽  
Richard Bendall ◽  
William Gaze ◽  
Lihong Zhang ◽  
...  
Keyword(s):  
Routine Laboratory ◽  
Simple Scheme ◽  
Diagnostic Laboratory ◽  
Laboratory Methods ◽  
Staphylococcus Pseudintermedius ◽  
Content Type ◽  
Dog Bites ◽  
Zoonotic Pathogens ◽  
Staphylococcus Intermedius

TheStaphylococcus intermediusgroup (SIG) includes zoonotic pathogens traditionally associated with dog bites. We describe a simple scheme for improved detection of SIG using routine laboratory methods, report its effect on isolation rates, and use sequencing to confirm that, apart from one atypical SIG strain, most isolates areStaphylococcus pseudintermedius.

scholarly journals DIFFERENCES IN CHANGES OF HEMOGLOBIN BETWEEN 6-12 HOURS AND 12-14 HOURS AFTER TRANSFUSION

2018 ◽  
Vol 24 (2) ◽  
pp. 108
Author(s):  
Rosita Linda ◽  
Devita Ninda
Keyword(s):  
Blood Transfusion ◽  
Secondary Data ◽  
Hemoglobin Concentration ◽  
Fresh Frozen Plasma ◽  
Blood Components ◽  
Blood Products ◽  
Fresh Frozen ◽  
Frozen Plasma ◽  
Hemoglobin Measurement ◽  
Baseline Hemoglobin

Each year more than 41,000 blood donations are needed every day and 30 million blood components are transfused. Blood products that can be transfused include Packed Red Cells (PRC), Whole Blood (WB), Thrombocyte Concentrate (TC), Fresh Frozen Plasma (FFP). Monitoring Hemoglobin (Hb) after transfusion is essential for assessing the success of a transfusion. The time factor after transfusion for Hemoglobin (Hb) examination needs to be established, analyze to judge the success of a blood transfusion which is performed. The aim of this study was to analyze the differences in changes of hemoglobin between 6-12 hours, and 12-24 hours after-transfusion. This study was retrospective observational using secondary data. The subjects were patients who received PRC, and WBC transfusion. At 6-12, and 12-24 hours after-transfusion, hemoglobin, RBC, and hematocrit were measured. Then the data were analyzed by unpaired t-test. The collected data included the results of the Hb pre-transfusion, 6-12, and 12-24 hours after-transfusion. The subjects of this study were 98 people. The administration of transfusion increased by 10-30% in hemoglobin concentration at 6-12 hours after-transfusion. While at 12-24 hours after-transfusion, hemoglobin after-transfusion increased 15-37% from the baseline. Hemoglobin values were not different at any of the defined after-transfusion times (p = 0.76 (p>0.05)). Hemoglobin values were not different at 6-12 hours, and 12-24 hours after-transfusion.    Keywords: Hemoglobin, measurement, after-transfusion 

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