STUDY TO COMPARE THE CORRELATION BETWEEN MOKSHIT DIGITAL HEMOGLOBINOMETER AND KT6400 HEMATOLOGY ANALYZER FOR HEMOGLOBIN MEASUREMENT

Anemia is a major health concern in India since many decades and is continue to rise. Therefore, Hemoglobin measurement is performed in various Pathology labs, Hospitals, Blood Banks etc. to detect Technology. Different method are used for Hemoglobin measurement like Automated Hematology Analyzer, copper sulphate gravimetric method, Hemoglobin colour scale, HICN method, point of care Hemoglobin meter etc.

A New Detection Chamber Design on Centrifugal Microfluidic Platform to Measure Hemoglobin of Whole Blood

Undoubtedly, microfluidics has been a focal point of interdisciplinary science during the last two decades, resulting in many developments in this area. Centrifugal microfluidic platforms have good potential for use in point-of-care devices because they take advantage of some intrinsic forces, most notably centrifugal force, which obviates the need to any external driving forces. Herein, we introduce a newly designed detection chamber for use on microfluidic discs that can be employed as an absorbance readout step in cases where the final solution has a very low viscosity and surface tension. In such situations, our chamber easily eliminates the air bubbles from the final solution without any interruption. One microfluidic disc for measuring the hemoglobin concentration was designed and constructed to verify the correct functioning of this detection chamber. This disc measured the hemoglobin concentration of the blood samples via the HiCN method. Then, the hemoglobin concentration of 11 blood samples was quantified and compared with the clinic’s data using the hemoglobin measurement disc, which included four hemoglobin measurement sets, and each set contained two inlets for the blood sample and the reagent, one two-part mixing chamber, and one bubble-free detection chamber. The measured values of the disc had good linearity and conformity compared with the clinic’s data, and there were no air bubbles in the detection step. In this study, the standard deviation and the turnaround time were ± 0.51 g/dL and 68 s, respectively.

Smart Non-Invasive Hemoglobin Measurement Using Portable Embedded Technology

Hemoglobin plays a major role for the existence of the healthy human being it carries oxygen supply to every cell for its survival in the entire body. As its level varies from person to person between 12-17 grams per deciliter if very high or low cause diseases. Usually low hemoglobin cause anemia in many of the women during pregnancy. In the present article the hemoglobin measurement is done noninvasively using the photoplethysmography method at the fingertip by Infrared LED with a wavelength of (880 – 940) nm and Red LED with wavelength of 660nm. The empirical calculation of Hemoglobin estimation is carried indirectly through model of oxygen saturation in skin-tissue- bone attenuation of the light on to the extremities. The analysis is supported by the Arduino IDE and MATLAB toolboxes for filtering the noise in signal and processing for target parameters. Further these real time parameters were sent to expertise to check for corrective ness and with the actual invasive methods for diagnosing its course of action. Internet of Things (IOT) technology to the outside world communication through Android mobile App.

Comparison of Arterial Blood Gas Analysis versus Central Laboratory Electrolyte and Hemoglobin Determination in Birat Medical College and Teaching Hospital

Introduction: Electrolyte and hemoglobin measurement are the integral part of management of critically ill patient. There can be a wide variation in the electrolyte and hemoglobin measurement in critically ill patient between arterial blood gas analyzer and central laboratory auto analyzer. Objective: To compare the electrolytes (sodium, potassium and chloride) and hemoglobin level measured by arterial blood gas analyzer and laboratory analyzer. Methodology: This was a prospective cross-sectional comparative study comparing the electrolytes (sodium, potassium and chloride) and hemoglobin measurement between arterial blood gas analyzer and laboratory auto analyzer. The study included 124 paired blood samples from the patient admitted in intensive care unit of Birat Medical College Teaching Hospital in two months duration. The arterial sample and venous sample for electrolytes and hemoglobin measurement were taken simultaneously or not more than one hour apart and analysis was done by arterial blood gas analyzer and central laboratory auto analyzer accordingly. The values of electrolytes and hemoglobin measured by two different analyzers were finally compared for variation. Result: The mean difference calculated for sodium potassium and chloride in ABG machine and Auto-analyzers were 0.57 mmol/l.-0.04mmol/l and 1.71mmol/l respectively. These data were within the acceptable range of United States Clinical Laboratory Improvement Amendments(USCLIA). The mean difference derived for hemoglobin in ABG and Auto-analyzers was 0.16g/dl which was not consistent with the range of United States Clinical Laboratory Improvement Amendments (USCLIA) Conclusion: The measurement of electrolyte namely sodium, potassium and chloride in ABG machines and Auto-analyzers of central lab were comparable while hemoglobin was not comparable under the USCLIA guidelines.