scholarly journals Identification of sequences encoding for ncr-peptides and defensins in the ‘meta-assembly’ of transcriptome of pea (pisum sativum l.) Nitrogen-fixing nodules

2019 ◽  
Vol 17 (3) ◽  
pp. 39-46 ◽  
Author(s):  
Evgeny A. Zorin ◽  
Marina S. Kliukova ◽  
Olga A. Kulaeva ◽  
Alexey M. Afonin ◽  
Igor A. Tikhonovich ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pisum Sativum ◽  
Signal Peptidase ◽  
Nitrogen Fixing ◽  
Antimicrobial Drugs ◽  
Pisum Sativum L ◽  
Plant Defensins ◽  
New Antibiotics ◽  
Emergence Of Resistance

Background. The active and careless applying of antibiotics in medicine and agriculture leads to the emergence of resistance to the existing antimicrobial drugs, which reduces the effectiveness of their use. One of the ways to solve this problem is the development of new antibiotics based on plant peptides with antimicrobial activity, for example plant defensins (which identified in all plants) and NCR peptides that are specifically synthesized in nodules of some leguminous plants. Materials and methods. In the present study, a meta-assembly of a transcriptome was constructed based on publicly available RNA-sequencing transcriptomes of pea nodules (Pisum sativum L.). This meta-assembly was used to search for sequences encoding antimicrobial peptides. Results. As a result, 55 and 908 unique sequences encoding defensins and NCR peptides, respectively, were identified. The recognition site for the signal peptidase was predicted and sequences were divided into the signal and mature part of the peptide. Among mature defensins, 22 peptides possess in silico predicted antimicrobial activity, and for the NCR peptides family their number was 422. Conclusion. Sequences encoding defensins and NCR peptides expressed in nitrogen-fixing pea nodules were identified. They are candidates for testing their antimicrobial activity in vitro.

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals Structural characterizations and in vitro digestibility of acid-treated wrinkled and smooth pea starch (Pisum sativum L.)

2016 ◽  
Vol 68 (7-8) ◽  
pp. 762-770 ◽  
Author(s):  
Miaomiao Shi ◽  
Kai Wang ◽  
Shujuan Yu ◽  
Robert G. Gilbert ◽  
Qunyu Gao
Keyword(s):  
Pisum Sativum ◽  
In Vitro Digestibility ◽  
Pisum Sativum L ◽  
Pea Starch ◽  
Structural Characterizations

Effect of the Drying Temperature on Color, Antioxidant Activity and In Vitro Digestibility of Green Pea ( Pisum sativum L.) Flour

2020 ◽  
Vol 72 (9-10) ◽  
pp. 1900228 ◽  
Author(s):  
Manolo Gonzalez ◽  
Jose Alvarez‐Ramirez ◽  
E. Jaime Vernon‐Carter ◽  
Isabel Reyes ◽  
Lurdes Alvarez‐Poblano
Keyword(s):  
Antioxidant Activity ◽  
Pisum Sativum ◽  
In Vitro Digestibility ◽  
Drying Temperature ◽  
Pisum Sativum L ◽  
Green Pea

Antimicrobial Activity of Differently Concentrated Nanoparticle Dispersions

2017 ◽  
Vol 13 ◽  
pp. 239-247 ◽  
Author(s):  
Georgy A. Frolov ◽  
Yakov N. Karasenkov ◽  
Alexander A. Gusev ◽  
Olga V. Zakharova ◽  
Anna Yu. Godymchuk ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Dental Plaque ◽  
Dispersion Medium ◽  
Antimicrobial Properties ◽  
Dispersed Phase ◽  
Colloidal Solutions ◽  
Nanoparticle Dispersions ◽  
New Antibiotics

Preparation of nontoxic antimicrobial solutions is a priority in the development of new antibiotics. The in vitro effect of the chemical composition of the dispersed phase and dissolved solids in the dispersion medium on the antimicrobial properties of aqueous colloidal solutions of nanoparticles of a number of metals and metal oxides against microorganisms of different species - bacterial dental plaque, Bacillus cereus, and spores of vaccine strain Bacillus anthracis STI - 1 were studied in this work.

Morphogenesis of the compound leaf in three genotypes of the pea, Pisum sativum

1986 ◽  
Vol 64 (6) ◽  
pp. 1268-1276 ◽  
Author(s):  
K. S. Gould ◽  
Elizabeth G. Cutter ◽  
J. P. W. Young
Keyword(s):  
Pisum Sativum ◽  
Leaf Development ◽  
Culture System ◽  
Algebraic Model ◽  
Leaf Primordium ◽  
Axillary Shoots ◽  
Compound Leaf ◽  
Pisum Sativum L ◽  
Leaflet Anatomy

Leaf anatomy, ontogeny, and morphology were described and compared in a pea line (Pisum sativum L.) with conventional leaves and in isogenic lines carrying the mutations af (afila) or tl (tendril-less or acacia). The anatomy of stem, petiole, and rachis is not modified by these mutations. The tendrils, which in af replace leaflets, have normal tendril anatomy, and the terminal leaflets of the tl form have normal leaflet anatomy. The shoot apical dome has the same size and shape in the three genotypes, as does the leaf primordium up to the stage of initiation of the first laterals. The mature morphology of leaves varies with node of insertion. Some leaves, especially at nodes 3 and 4, have structures that are not typical of their genotype. An in vitro culture system is described for axillary shoots. Such shoots recapitulate most of the foliar features of seedling plants, but leaf morphology is on average more complex, and aberrant structures are more frequent. All these observations are discussed in relation to Young's algebraic model for compound leaf development.

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