scholarly journals Double Mutant Analysis of Sequential Functioning of Pea (Pisum sativum L.) Genes: Sym13, Sym33 and Sym40 During Symbiotic Nodule Development

2010 ◽  
Vol 8 (2) ◽  
pp. 3-8 ◽  
Author(s):  
Viktor E Tsyganov ◽  
Elena V Seliverstova ◽  
Vera A Voroshilova ◽  
Anna V Tsyganova ◽  
Zlata B Pavlova ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Mutant Allele ◽  
Double Mutant ◽  
Developmental Stages ◽  
Infection Process ◽  
Nodule Development ◽  
Symbiotic Gene ◽  
Mutant Analysis ◽  
Pisum Sativum L ◽  
Symbiotic Nodule

Two double mutants carrying pea symbiotic gene pairs sym13, sym40 and sym33, sym40, respectively, were constructed using single mutants blocked at different nodule developmental stages: E135f (sym13), SGEFix–‑1 (sym40) and SGEFix–‑2 (sym33). The epistasis of the mutant allele sym40 over the mutant allele sym13 and sym33 over sym40 was shown with respect to nodule histological and ultrastructural organisation. Thus, the proposed earlier sequential functioning of genes during infection process: Sym33→Sym40→Sym13 has been confirmed. 

scholarly journals ANALYSIS OF INTERACTION OF PEA (Pisum sativum L.) SYMBIOTIC GENES Sym33 AND Sym42 WHOSE MUTATIONS RESULT IN ABNORMALITES DURING INFECTION THREAD DEVELOPMENT

2012 ◽  
Vol 10 (4) ◽  
pp. 50-55
Author(s):  
Viktor E Tsyganov ◽  
Anna V Tsyganova ◽  
Vera A Voroshilova ◽  
Aleksey U Borisov ◽  
Igor A Tikhonovich
Keyword(s):  
Pisum Sativum ◽  
Mutant Allele ◽  
Double Mutant ◽  
Nodule Development ◽  
Symbiotic Genes ◽  
Pisum Sativum L ◽  
Infection Threads ◽  
Gene Functions ◽  
Symbiotic Nodule ◽  
Mutant Lines

Using pea single mutant lines SGEFix–-2 (sym33) and RisFixV (sym42), which are characterized by different abnormalities during symbiotic nodule development, including thickening of infection threads’ walls, a double mutant RBT4 line, carrying a pair of symbiotic genes sym33 and sym42 was constructed. The epistasis of the mutant allele sym33 over the mutant allele sym42 with respect to the histological and ultrastructural organisation of nodules was shown. Thus, it was demonstrated that Sym33 gene functions earlier in symbiotic nodule development than Sym42 gene.

Double mutant analysis of sequential functioning of pea (Pisum sativum L.) genes Sym13, Sym33, and Sym40 during symbiotic nodule development

2011 ◽  
Vol 1 (5) ◽  
pp. 343-348 ◽  
Author(s):  
V. E. Tsyganov ◽  
E. V. Seliverstova ◽  
V. A. Voroshilova ◽  
A. V. Tsyganova ◽  
Z. B. Pavlova ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Double Mutant ◽  
Nodule Development ◽  
Mutant Analysis ◽  
Pisum Sativum L ◽  
Symbiotic Nodule

scholarly journals Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development

2017 ◽  
Vol 9 (3) ◽  
pp. 22-35 ◽  
Author(s):  
E. A. Dolgikh ◽  
A. I. Shaposhnikov ◽  
A. V. Dolgikh ◽  
E. S. Gribchenko ◽  
K. B. Bodyagina ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Nodule Development ◽  
Pisum Sativum L ◽  
Symbiotic Nodule

Four developmental stages identified by genetic dissection of pea (Pisum sativum L.) root nodule morphogenesis

Plant Science ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Elena V Morzhina ◽  
Viktor E Tsyganov ◽  
Alexey Y Borisov ◽  
Vladimir K Lebsky ◽  
Igor A Tikhonovich
Keyword(s):  
Pisum Sativum ◽  
Root Nodule ◽  
Developmental Stages ◽  
Genetic Dissection ◽  
Pisum Sativum L

scholarly journals Regulation of the Later Stages of Nodulation Stimulated by IPD3/CYCLOPS Transcription Factor and Cytokinin in Pea Pisum sativum L.

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 56
Author(s):  
Elizaveta S. Rudaya ◽  
Polina Yu. Kozyulina ◽  
Olga A. Pavlova ◽  
Alexandra V. Dolgikh ◽  
Alexandra N. Ivanova ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Comparative Analysis ◽  
Pisum Sativum ◽  
Nodule Development ◽  
Rna Seq ◽  
Wild Type ◽  
Development Stages ◽  
Pisum Sativum L ◽  
Pea Mutant

The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and ipd3/cyclops mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix--2 (sym33) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin’s influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix--2 (sym33) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.

scholarly journals The formation of symbiotic interface in root nodules of Pisum sativum L. and Medicago truncatula Gaertn

2020 ◽  
Author(s):  
A. V. Tsyganova ◽  
E. V. Seliverstova ◽  
N. J. Brewin ◽  
V. E. Tsyganov
Keyword(s):  
Pisum Sativum ◽  
Medicago Truncatula ◽  
Root Nodules ◽  
Nodule Development ◽  
Root Cells ◽  
Pisum Sativum L ◽  
Species Specific

The infection of root cells of legumes with rhizobia involves the gradual remodelling of the plant-microbial interface. General and species-specific features of symbiotic interface remodelling during nodule development were demonstrated.

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